Differences in membrane electrical properties between C3H 10T1/2 mouse embryo fibroblasts and their ionizing radiation and chemically transformed counterparts
Membrane electrical properties of mouse embryo fibroblasts and their ionizing radiation and chemically transformed counterparts were investigated using dielectric relaxation measurements in the radio frequency range. This determination is possible because, in the radio frequency range, suspensions o...
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| Veröffentlicht in: | European biophysics journal 1992, Vol.20 (6), p.305-309 |
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| container_issue | 6 |
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| container_title | European biophysics journal |
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| creator | SANTINI, M. T CAMETTI, C BONINCONTRO, A NAPOLITANO, M INDOVINA, P. L DURANTE, M GIALANELLA, G GROSSI, G. F |
| description | Membrane electrical properties of mouse embryo fibroblasts and their ionizing radiation and chemically transformed counterparts were investigated using dielectric relaxation measurements in the radio frequency range. This determination is possible because, in the radio frequency range, suspensions of cells in an electrolyte buffer show a conductivity dispersion due to interfacial polarization. An analysis of the experimental data based on a "single-shell" model showed that conductivity and permittivity of the membranes of both radiation and chemically transformed fibroblasts were lower than in normal cells. In addition, the conductivity of the cytoplasm was higher in both transformed cell types than in the normal mouse fibroblasts. We discuss the significance of these findings in view of the possible structural and functional modifications brought about by the process of neoplastic transformation. |
| doi_str_mv | 10.1007/BF00196589 |
| format | Article |
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In addition, the conductivity of the cytoplasm was higher in both transformed cell types than in the normal mouse fibroblasts. We discuss the significance of these findings in view of the possible structural and functional modifications brought about by the process of neoplastic transformation.</description><identifier>ISSN: 0175-7571</identifier><identifier>EISSN: 1432-1017</identifier><identifier>DOI: 10.1007/BF00196589</identifier><identifier>PMID: 1559503</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Buffers ; Cell Line, Transformed - drug effects ; Cell Line, Transformed - physiology ; Cell Line, Transformed - radiation effects ; Cell Membrane - drug effects ; Cell Membrane - physiology ; Cell Membrane - radiation effects ; Cell Membrane Permeability - physiology ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cell Transformation, Neoplastic - drug effects ; Cell Transformation, Neoplastic - radiation effects ; Cytoplasm - physiology ; Fibroblasts - drug effects ; Fibroblasts - physiology ; Fibroblasts - radiation effects ; Fundamental and applied biological sciences. Psychology ; Membrane Potentials - drug effects ; Membrane Potentials - radiation effects ; Methylcholanthrene - pharmacology ; Mice ; Mice, Inbred C3H ; Molecular and cellular biology ; Solutions - chemistry</subject><ispartof>European biophysics journal, 1992, Vol.20 (6), p.305-309</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-53c26879f103157ffb2d5066e4952e717a7f1c2e6a17d92c321b2512f0eeea953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5194123$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1559503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SANTINI, M. 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An analysis of the experimental data based on a "single-shell" model showed that conductivity and permittivity of the membranes of both radiation and chemically transformed fibroblasts were lower than in normal cells. In addition, the conductivity of the cytoplasm was higher in both transformed cell types than in the normal mouse fibroblasts. We discuss the significance of these findings in view of the possible structural and functional modifications brought about by the process of neoplastic transformation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Cell Line, Transformed - drug effects</subject><subject>Cell Line, Transformed - physiology</subject><subject>Cell Line, Transformed - radiation effects</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - physiology</subject><subject>Cell Membrane - radiation effects</subject><subject>Cell Membrane Permeability - physiology</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cell Transformation, Neoplastic - drug effects</subject><subject>Cell Transformation, Neoplastic - radiation effects</subject><subject>Cytoplasm - physiology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - physiology</subject><subject>Fibroblasts - radiation effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - radiation effects</subject><subject>Methylcholanthrene - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Molecular and cellular biology</subject><subject>Solutions - chemistry</subject><issn>0175-7571</issn><issn>1432-1017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUtPQyEQhYnRaH1s3JuwMC5MrmXgcilLrc-kiRtd33DpoJj7qEBj6o_xt4q20Q2EOR8zOWcIOQZ2AYyp8dUtY6ArOdFbZASl4AUwUNtklE9ZKKlgj-zH-MZYKQEmu2QXpNSSiRH5uvbOYcDeYqS-px12TTA9UmzRpuCtaekiDAsMyWeiwfSB2NOpuKfAnmDMaTcsY8bzt9VAnW_C0LQmpkhNP6fpFX2gfuj9p-9faDBzb1J-_or2FbufAe2KpjwzuiF0mMvDsk8YFiakeEh2nGkjHm3uA_J8e_M0vS9mj3cP08tZYQVAKqSwvJoo7YAJkMq5hs8lqyosteSoQBnlwHKsDKi55lZwaLgE7hgiGi3FATlb981e35cYU935aLFtcxTZX634RIlS8wyer0EbhhgDunoRfGfCqgZW_yyj_l9Ghk82XZdNdvaPrtPP-ulGNzHn4HII1sc_TIIugQvxDQ-Ikw0</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>SANTINI, M. 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Action of oncogenes and antioncogenes</topic><topic>Cell Transformation, Neoplastic - drug effects</topic><topic>Cell Transformation, Neoplastic - radiation effects</topic><topic>Cytoplasm - physiology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - physiology</topic><topic>Fibroblasts - radiation effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - radiation effects</topic><topic>Methylcholanthrene - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Molecular and cellular biology</topic><topic>Solutions - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SANTINI, M. T</creatorcontrib><creatorcontrib>CAMETTI, C</creatorcontrib><creatorcontrib>BONINCONTRO, A</creatorcontrib><creatorcontrib>NAPOLITANO, M</creatorcontrib><creatorcontrib>INDOVINA, P. 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F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in membrane electrical properties between C3H 10T1/2 mouse embryo fibroblasts and their ionizing radiation and chemically transformed counterparts</atitle><jtitle>European biophysics journal</jtitle><addtitle>Eur Biophys J</addtitle><date>1992</date><risdate>1992</risdate><volume>20</volume><issue>6</issue><spage>305</spage><epage>309</epage><pages>305-309</pages><issn>0175-7571</issn><eissn>1432-1017</eissn><abstract>Membrane electrical properties of mouse embryo fibroblasts and their ionizing radiation and chemically transformed counterparts were investigated using dielectric relaxation measurements in the radio frequency range. This determination is possible because, in the radio frequency range, suspensions of cells in an electrolyte buffer show a conductivity dispersion due to interfacial polarization. An analysis of the experimental data based on a "single-shell" model showed that conductivity and permittivity of the membranes of both radiation and chemically transformed fibroblasts were lower than in normal cells. In addition, the conductivity of the cytoplasm was higher in both transformed cell types than in the normal mouse fibroblasts. We discuss the significance of these findings in view of the possible structural and functional modifications brought about by the process of neoplastic transformation.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>1559503</pmid><doi>10.1007/BF00196589</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Buffers Cell Line, Transformed - drug effects Cell Line, Transformed - physiology Cell Line, Transformed - radiation effects Cell Membrane - drug effects Cell Membrane - physiology Cell Membrane - radiation effects Cell Membrane Permeability - physiology Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cell Transformation, Neoplastic - drug effects Cell Transformation, Neoplastic - radiation effects Cytoplasm - physiology Fibroblasts - drug effects Fibroblasts - physiology Fibroblasts - radiation effects Fundamental and applied biological sciences. Psychology Membrane Potentials - drug effects Membrane Potentials - radiation effects Methylcholanthrene - pharmacology Mice Mice, Inbred C3H Molecular and cellular biology Solutions - chemistry |
| title | Differences in membrane electrical properties between C3H 10T1/2 mouse embryo fibroblasts and their ionizing radiation and chemically transformed counterparts |
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