Fiber optic multi-channel protein detector for use in preparative continuous annular chromatography
Continuous annular chromatography is an effective method in the separation of preparative scale quantities of biological compounds including proteins where established batch chromatography borders on it. The need for identification or quantification of proteins triggered the development of respectiv...
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| Veröffentlicht in: | Journal of Chromatography A 2002-08, Vol.967 (2), p.183-189 |
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| container_end_page | 189 |
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| container_issue | 2 |
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| container_title | Journal of Chromatography A |
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| creator | Apostolidis, Athanasios Lehmann, Hartmut Schwotzer, Günter Willsch, Reinhardt Prior, Albert Wolfgang, Jürgen Klimant, Ingo Wolfbeis, Otto S |
| description | Continuous annular chromatography is an effective method in the separation of preparative scale quantities of biological compounds including proteins where established batch chromatography borders on it. The need for identification or quantification of proteins triggered the development of respective detection units. Here, we describe two types of optical multi-channel detectors. The first is a fiber optic multi-channel detector suitable for the separation of aqueous protein solutions. The second is a technically improved (circular optic) device suitable for application in multi-channel detection. Specifically, UV-absorption measurements of proteins at 280 nm were carried out using newly designed fiber optic detectors having eight and 16 channels. Calibration plots were established for a series of stock solutions of known concentrations of proteins. Mathematical functions were derived from these calibration data to simulate the response of the detector. Limits of detection and the ranges of validity of the fit functions were determined. The 16-channel detector has a theoretical limit of detection that is equivalent to absorbance changes of 10
−4 units. |
| doi_str_mv | 10.1016/S0021-9673(02)00755-0 |
| format | Article |
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−4 units.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromatography, Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Methods. Procedures. 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| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Biotechnology Chromatography, Liquid - methods Fundamental and applied biological sciences. Psychology General aspects, investigation methods Methods. Procedures. Technologies Others Proteins Proteins - isolation & purification Spectrophotometry, Ultraviolet Various methods and equipments |
| title | Fiber optic multi-channel protein detector for use in preparative continuous annular chromatography |
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