A Raised Level of Soluble CD8 in Bronchoalveolar Lavage Fluid in Summer-Type Hypersensitivity Pneumonitis in Japan
We used ELISA to measure soluble CD8 (sCD8) in the bronchoalveolar lavage fluid (BALF) and serum of patients with summer-type hypersensitivity pneumonitis (HP). The sCD8 levels in BALF were significantly higher in the patients with summer-type HP, surpassing those found in sarcoidosis and the other...
Gespeichert in:
| Veröffentlicht in: | Chest 1992-04, Vol.101 (4), p.1044-1049 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1049 |
|---|---|
| container_issue | 4 |
| container_start_page | 1044 |
| container_title | Chest |
| container_volume | 101 |
| creator | Hamagami, Sayo Miyagawa, Toshi Ochi, Tadao Tsuyuguchi, Izuo Kishimoto, Susumu |
| description | We used ELISA to measure soluble CD8 (sCD8) in the bronchoalveolar lavage fluid (BALF) and serum of patients with summer-type hypersensitivity pneumonitis (HP). The sCD8 levels in BALF were significantly higher in the patients with summer-type HP, surpassing those found in sarcoidosis and the other pulmonary diseases studied; however, the sCD8 levels in the serum of patients with summer-type HP did not differ from the levels of the healthy controls. The numbers of CD8+ T cells were increased in the BALF of the patients with summer-type HP, and there was a correlation between the sCD8 levels and the concentrations of CD8+ T cells. Gel filtration and polyacrylamide gel electrophoresis of the fluid revealed that the anti-CD8 monoclonal antibody-reactive components in the BALF of patients with pneumonitis corresponded to a protein with a molecular weight of between 52 and 54 kDa. Soluble CD8-rich fraction purified from the BALF of patients with summer-type HP augmented in vitro lymphocytes' proliferative responses stimulated with Cryptococcus neoformans, one of the causative agents for summer-type HP. Our result suggests that soluble CD8 in the BALF may play an important role in the pathogenesis of summer-type HP. |
| doi_str_mv | 10.1378/chest.101.4.1044 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72870104</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S001236921632726X</els_id><sourcerecordid>72870104</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-8f099503e86ff2a3f05d583961f0db89d0aab700162cc3bafc392ceed40055593</originalsourceid><addsrcrecordid>eNp1kM1v1DAQxS0EKtvCnQuSD4hbFjvOl7mVhVLQSiBazpZjj7uuHHuxk6D97-slKyoOXMYazXvzPD-EXlGypqzt3qkdpHFNCV1XuVbVE7SinNGC1RV7ilaE0LJgDS-fo_OU7knuKW_O0Bmt67qifIXiJf4hbQKNtzCDw8Hgm-Cm3gHefOyw9fhDDF7tgnQzBCcj3spZ3gG-cpPVx_nNNAwQi9vDHvB1LjGBT3a0sx0P-LuHaQg-t-mo_Sr30r9Az4x0CV6e3gv08-rT7ea62H77_GVzuS1UReux6AzhvCYMusaYUjJDal13jDfUEN13XBMp-zZf1JRKsV4axXipAHRFSL6Oswv0dtm7j-HXlDmJwSYFzkkPYUqiLbuWZGhZSBahiiGlCEbsox1kPAhKxBGz-IM5d1RU4og5W16fdk_9APrRsHDN8zenuUxKOhOlVzb9ldVl27ake0ze2bvdbxtBpEE6l5eyJfM-TNFL90_y-8UCGd1sIYqkLHgFOtvVKHSw___2A2-xq34</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72870104</pqid></control><display><type>article</type><title>A Raised Level of Soluble CD8 in Bronchoalveolar Lavage Fluid in Summer-Type Hypersensitivity Pneumonitis in Japan</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Hamagami, Sayo ; Miyagawa, Toshi ; Ochi, Tadao ; Tsuyuguchi, Izuo ; Kishimoto, Susumu</creator><creatorcontrib>Hamagami, Sayo ; Miyagawa, Toshi ; Ochi, Tadao ; Tsuyuguchi, Izuo ; Kishimoto, Susumu</creatorcontrib><description>We used ELISA to measure soluble CD8 (sCD8) in the bronchoalveolar lavage fluid (BALF) and serum of patients with summer-type hypersensitivity pneumonitis (HP). The sCD8 levels in BALF were significantly higher in the patients with summer-type HP, surpassing those found in sarcoidosis and the other pulmonary diseases studied; however, the sCD8 levels in the serum of patients with summer-type HP did not differ from the levels of the healthy controls. The numbers of CD8+ T cells were increased in the BALF of the patients with summer-type HP, and there was a correlation between the sCD8 levels and the concentrations of CD8+ T cells. Gel filtration and polyacrylamide gel electrophoresis of the fluid revealed that the anti-CD8 monoclonal antibody-reactive components in the BALF of patients with pneumonitis corresponded to a protein with a molecular weight of between 52 and 54 kDa. Soluble CD8-rich fraction purified from the BALF of patients with summer-type HP augmented in vitro lymphocytes' proliferative responses stimulated with Cryptococcus neoformans, one of the causative agents for summer-type HP. Our result suggests that soluble CD8 in the BALF may play an important role in the pathogenesis of summer-type HP.</description><identifier>ISSN: 0012-3692</identifier><identifier>EISSN: 1931-3543</identifier><identifier>DOI: 10.1378/chest.101.4.1044</identifier><identifier>PMID: 1555419</identifier><identifier>CODEN: CHETBF</identifier><language>eng</language><publisher>Northbrook, IL: Elsevier Inc</publisher><subject>Adult ; Aged ; Allergic diseases ; Alveolitis, Extrinsic Allergic - etiology ; Alveolitis, Extrinsic Allergic - immunology ; Antigens, Bacterial - immunology ; Biological and medical sciences ; Bronchoalveolar Lavage Fluid - immunology ; CD8 Antigens - analysis ; CD8 Antigens - immunology ; Chromatography, Gel ; Cryptococcus neoformans - immunology ; Electrophoresis, Polyacrylamide Gel ; Female ; Fluorescent Antibody Technique ; Humans ; Immunopathology ; Japan ; Leukocytes, Mononuclear - immunology ; Male ; Medical sciences ; Middle Aged ; Molecular Weight ; Respiratory and ent allergic diseases ; Seasons ; Solubility</subject><ispartof>Chest, 1992-04, Vol.101 (4), p.1044-1049</ispartof><rights>1992 The American College of Chest Physicians</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-8f099503e86ff2a3f05d583961f0db89d0aab700162cc3bafc392ceed40055593</citedby><cites>FETCH-LOGICAL-c415t-8f099503e86ff2a3f05d583961f0db89d0aab700162cc3bafc392ceed40055593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5277708$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1555419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamagami, Sayo</creatorcontrib><creatorcontrib>Miyagawa, Toshi</creatorcontrib><creatorcontrib>Ochi, Tadao</creatorcontrib><creatorcontrib>Tsuyuguchi, Izuo</creatorcontrib><creatorcontrib>Kishimoto, Susumu</creatorcontrib><title>A Raised Level of Soluble CD8 in Bronchoalveolar Lavage Fluid in Summer-Type Hypersensitivity Pneumonitis in Japan</title><title>Chest</title><addtitle>Chest</addtitle><description>We used ELISA to measure soluble CD8 (sCD8) in the bronchoalveolar lavage fluid (BALF) and serum of patients with summer-type hypersensitivity pneumonitis (HP). The sCD8 levels in BALF were significantly higher in the patients with summer-type HP, surpassing those found in sarcoidosis and the other pulmonary diseases studied; however, the sCD8 levels in the serum of patients with summer-type HP did not differ from the levels of the healthy controls. The numbers of CD8+ T cells were increased in the BALF of the patients with summer-type HP, and there was a correlation between the sCD8 levels and the concentrations of CD8+ T cells. Gel filtration and polyacrylamide gel electrophoresis of the fluid revealed that the anti-CD8 monoclonal antibody-reactive components in the BALF of patients with pneumonitis corresponded to a protein with a molecular weight of between 52 and 54 kDa. Soluble CD8-rich fraction purified from the BALF of patients with summer-type HP augmented in vitro lymphocytes' proliferative responses stimulated with Cryptococcus neoformans, one of the causative agents for summer-type HP. Our result suggests that soluble CD8 in the BALF may play an important role in the pathogenesis of summer-type HP.</description><subject>Adult</subject><subject>Aged</subject><subject>Allergic diseases</subject><subject>Alveolitis, Extrinsic Allergic - etiology</subject><subject>Alveolitis, Extrinsic Allergic - immunology</subject><subject>Antigens, Bacterial - immunology</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - immunology</subject><subject>CD8 Antigens - analysis</subject><subject>CD8 Antigens - immunology</subject><subject>Chromatography, Gel</subject><subject>Cryptococcus neoformans - immunology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>Japan</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Weight</subject><subject>Respiratory and ent allergic diseases</subject><subject>Seasons</subject><subject>Solubility</subject><issn>0012-3692</issn><issn>1931-3543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1v1DAQxS0EKtvCnQuSD4hbFjvOl7mVhVLQSiBazpZjj7uuHHuxk6D97-slKyoOXMYazXvzPD-EXlGypqzt3qkdpHFNCV1XuVbVE7SinNGC1RV7ilaE0LJgDS-fo_OU7knuKW_O0Bmt67qifIXiJf4hbQKNtzCDw8Hgm-Cm3gHefOyw9fhDDF7tgnQzBCcj3spZ3gG-cpPVx_nNNAwQi9vDHvB1LjGBT3a0sx0P-LuHaQg-t-mo_Sr30r9Az4x0CV6e3gv08-rT7ea62H77_GVzuS1UReux6AzhvCYMusaYUjJDal13jDfUEN13XBMp-zZf1JRKsV4axXipAHRFSL6Oswv0dtm7j-HXlDmJwSYFzkkPYUqiLbuWZGhZSBahiiGlCEbsox1kPAhKxBGz-IM5d1RU4og5W16fdk_9APrRsHDN8zenuUxKOhOlVzb9ldVl27ake0ze2bvdbxtBpEE6l5eyJfM-TNFL90_y-8UCGd1sIYqkLHgFOtvVKHSw___2A2-xq34</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>Hamagami, Sayo</creator><creator>Miyagawa, Toshi</creator><creator>Ochi, Tadao</creator><creator>Tsuyuguchi, Izuo</creator><creator>Kishimoto, Susumu</creator><general>Elsevier Inc</general><general>American College of Chest Physicians</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920401</creationdate><title>A Raised Level of Soluble CD8 in Bronchoalveolar Lavage Fluid in Summer-Type Hypersensitivity Pneumonitis in Japan</title><author>Hamagami, Sayo ; Miyagawa, Toshi ; Ochi, Tadao ; Tsuyuguchi, Izuo ; Kishimoto, Susumu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-8f099503e86ff2a3f05d583961f0db89d0aab700162cc3bafc392ceed40055593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Allergic diseases</topic><topic>Alveolitis, Extrinsic Allergic - etiology</topic><topic>Alveolitis, Extrinsic Allergic - immunology</topic><topic>Antigens, Bacterial - immunology</topic><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - immunology</topic><topic>CD8 Antigens - analysis</topic><topic>CD8 Antigens - immunology</topic><topic>Chromatography, Gel</topic><topic>Cryptococcus neoformans - immunology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>Japan</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Weight</topic><topic>Respiratory and ent allergic diseases</topic><topic>Seasons</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamagami, Sayo</creatorcontrib><creatorcontrib>Miyagawa, Toshi</creatorcontrib><creatorcontrib>Ochi, Tadao</creatorcontrib><creatorcontrib>Tsuyuguchi, Izuo</creatorcontrib><creatorcontrib>Kishimoto, Susumu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chest</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamagami, Sayo</au><au>Miyagawa, Toshi</au><au>Ochi, Tadao</au><au>Tsuyuguchi, Izuo</au><au>Kishimoto, Susumu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Raised Level of Soluble CD8 in Bronchoalveolar Lavage Fluid in Summer-Type Hypersensitivity Pneumonitis in Japan</atitle><jtitle>Chest</jtitle><addtitle>Chest</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>101</volume><issue>4</issue><spage>1044</spage><epage>1049</epage><pages>1044-1049</pages><issn>0012-3692</issn><eissn>1931-3543</eissn><coden>CHETBF</coden><abstract>We used ELISA to measure soluble CD8 (sCD8) in the bronchoalveolar lavage fluid (BALF) and serum of patients with summer-type hypersensitivity pneumonitis (HP). The sCD8 levels in BALF were significantly higher in the patients with summer-type HP, surpassing those found in sarcoidosis and the other pulmonary diseases studied; however, the sCD8 levels in the serum of patients with summer-type HP did not differ from the levels of the healthy controls. The numbers of CD8+ T cells were increased in the BALF of the patients with summer-type HP, and there was a correlation between the sCD8 levels and the concentrations of CD8+ T cells. Gel filtration and polyacrylamide gel electrophoresis of the fluid revealed that the anti-CD8 monoclonal antibody-reactive components in the BALF of patients with pneumonitis corresponded to a protein with a molecular weight of between 52 and 54 kDa. Soluble CD8-rich fraction purified from the BALF of patients with summer-type HP augmented in vitro lymphocytes' proliferative responses stimulated with Cryptococcus neoformans, one of the causative agents for summer-type HP. Our result suggests that soluble CD8 in the BALF may play an important role in the pathogenesis of summer-type HP.</abstract><cop>Northbrook, IL</cop><pub>Elsevier Inc</pub><pmid>1555419</pmid><doi>10.1378/chest.101.4.1044</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-3692 |
| ispartof | Chest, 1992-04, Vol.101 (4), p.1044-1049 |
| issn | 0012-3692 1931-3543 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72870104 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Aged Allergic diseases Alveolitis, Extrinsic Allergic - etiology Alveolitis, Extrinsic Allergic - immunology Antigens, Bacterial - immunology Biological and medical sciences Bronchoalveolar Lavage Fluid - immunology CD8 Antigens - analysis CD8 Antigens - immunology Chromatography, Gel Cryptococcus neoformans - immunology Electrophoresis, Polyacrylamide Gel Female Fluorescent Antibody Technique Humans Immunopathology Japan Leukocytes, Mononuclear - immunology Male Medical sciences Middle Aged Molecular Weight Respiratory and ent allergic diseases Seasons Solubility |
| title | A Raised Level of Soluble CD8 in Bronchoalveolar Lavage Fluid in Summer-Type Hypersensitivity Pneumonitis in Japan |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T19%3A24%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Raised%20Level%20of%20Soluble%20CD8%20in%20Bronchoalveolar%20Lavage%20Fluid%20in%20Summer-Type%20Hypersensitivity%20Pneumonitis%20in%20Japan&rft.jtitle=Chest&rft.au=Hamagami,%20Sayo&rft.date=1992-04-01&rft.volume=101&rft.issue=4&rft.spage=1044&rft.epage=1049&rft.pages=1044-1049&rft.issn=0012-3692&rft.eissn=1931-3543&rft.coden=CHETBF&rft_id=info:doi/10.1378/chest.101.4.1044&rft_dat=%3Cproquest_cross%3E72870104%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72870104&rft_id=info:pmid/1555419&rft_els_id=S001236921632726X&rfr_iscdi=true |