Interaction of interferon-α with interleukin-1β or tumor necrosis factor-α on hepatitis B virus enhancer activity
The interaction of IFN-α with IL-1β or TNF-α on hepatitis B surface antigen (HBsAg) expression was analysed in hepatitis B virus (HBV)-DNA integrated PLC/PRF/5 and non-integrated HuH-7 human hepatoma cells. Secretion of HBsAg in PLC/PRF/5 cells was reduced by IFN-α, IL-1β or TNF-α, and synergistical...
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Veröffentlicht in: | Biochemical and biophysical research communications 1992-03, Vol.183 (2), p.904-909 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The interaction of IFN-α with IL-1β or TNF-α on hepatitis B surface antigen (HBsAg) expression was analysed in hepatitis B virus (HBV)-DNA integrated PLC/PRF/5 and non-integrated HuH-7 human hepatoma cells. Secretion of HBsAg in PLC/PRF/5 cells was reduced by IFN-α, IL-1β or TNF-α, and synergistically depressed when -α was used in combination with IL-1β or TNF-α. By Northern blot analysis, the levels of HBsAg mRNA were suppressed by IFN-α in combination with IL-1β or TNF-α. In the chloramphenicol acetyltransferase plasmid transfection assay, IFN-α in combination with IL-1β or TNF-α caused a much greater suppression of HBV enhancer activity than IFN-α, IL-1β or TNF-α alone in both hepatoma cells. These findings suggest that the interaction of IFN-α with IL-1β or TNF-α synergistically represses HBV enhancer activity, resulting in depressed expression of HBsAg. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(92)90569-7 |