Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column
To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column. The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count
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| Veröffentlicht in: | Fertility and sterility 1992-04, Vol.57 (4), p.866-870 |
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| container_title | Fertility and sterility |
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| creator | Ohashi, Kazutomo Saji, Fumitaka Wakimoto, Akinori Kato, Munehiro Tsutsui, Tateki Tanizawa, Osamu |
| description | To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column.
The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count |
| doi_str_mv | 10.1016/S0015-0282(16)54972-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72866395</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028216549723</els_id><sourcerecordid>72866395</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-8012716e6c425c8d9ae54c3cc2c53d3677e8feb917f7e9fbc91646ec0e69dfba3</originalsourceid><addsrcrecordid>eNqFUcFu1TAQtBCoPAqfUCmHCsEh1I5jOz6hqgJaqRJIhbPlt1kXo8R-tZNK7Q_0t3GaR-HGabWamZ3VDCFHjH5glMmTK0qZqGnTNe-YfC9arZqaPyMbJoSshRT8Odk8UV6SVzn_opRKppoDcsA441ypDXn4lnBnk518DFV0VRz8dbyPMe8wjR4qG_qTmCqbp58Y_gUyjhgqVzAfpmTnCZMPWJYC-LDem7MP11VRVleLarH6o_PDtDeFOMxjeE1eODtkfLOfh-TH50_fz87ry69fLs5OL2vgnZ7qjrJGMYkS2kZA12uLogUO0IDgPZdKYedwq5lyCrXbgmaylQgUpe7d1vJD8na9u0vxZsY8mdFnwGGwAeOcjWo6KbkWhShWIqSYc0JndsmPNt0ZRs1SgHkswCzpmrI9FmB40R3tDebtiP1f1Zp4wY_3uM1gB5dsAJ-faC1vdSsW-48rDUsYtx6TyeAxAPY-IUymj_4_j_wGnCemyA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72866395</pqid></control><display><type>article</type><title>Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>ScienceDirect Journals (5 years ago - present)</source><source>Alma/SFX Local Collection</source><creator>Ohashi, Kazutomo ; Saji, Fumitaka ; Wakimoto, Akinori ; Kato, Munehiro ; Tsutsui, Tateki ; Tanizawa, Osamu</creator><creatorcontrib>Ohashi, Kazutomo ; Saji, Fumitaka ; Wakimoto, Akinori ; Kato, Munehiro ; Tsutsui, Tateki ; Tanizawa, Osamu</creatorcontrib><description>To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column.
The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count<20×106/mL, sperm motility <40%). After concentration of motile sperm using a 40% Percoll density gradient centrifugation, the sperm suspension was filtered through the SpermPrep column. The percentage yield of motile sperm by the SpermPrep method was compared with those by a two-layer Percoll density gradient (Pharmacia, Uppsala, Sweden) centrifugation and a swim-up method. Infertile couples with poor quality semen were treated with intrauterine insemination (IUI) with motile sperm by the three preparations through three cycles.
Department of Obstetrics and Gynecology, Osaka University Hospital.
Twenty-one couples with long-standing infertility because of poor quality semen.
Recovery of motile sperm, sperm motility, and outcome of IUI were evaluated among three semen preparations.
Motility was improved by the SpermPrep method in 32 of 33 cases of oligozoospermia and/or asthenozoospermia. Percentage yield of motile sperm by the SpermPrep method was significantly greater than those by the two-layer Percoll density gradient and swim-up methods (42.7±4.6 versus 22.1±3.1 and 13.8±3.5), but there is no significant difference in the sperm motility among three semen preparations. After one treatment cycle for each preparation, 2 of 21 women conceived after IUI with motile sperm separated in the SpermPrep method.
The SpermPrep method is an improved semen manipulation method for oligozoospermia and/or asthenozoospermia.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(16)54972-3</identifier><identifier>PMID: 1313377</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>asthenozoospermia ; Biological and medical sciences ; Birth control ; Cell Separation ; Centrifugation, Density Gradient - methods ; Disposable Equipment ; Female ; Filtration - instrumentation ; Filtration - methods ; Gynecology. Andrology. Obstetrics ; Humans ; Insemination, Artificial, Homologous - instrumentation ; Insemination, Artificial, Homologous - methods ; intrauterine insemination ; Male ; Medical sciences ; Oligospermia - physiopathology ; oligozoospermia ; Percoll density gradient centrifugation ; Povidone ; Semen ; Silicon Dioxide ; Sperm Motility ; Spermatozoa - cytology ; SpermPrep semen filtration column ; Sterility. Assisted procreation ; swim-up method ; Uterus</subject><ispartof>Fertility and sterility, 1992-04, Vol.57 (4), p.866-870</ispartof><rights>1992 American Society for Reproductive Medicine</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-8012716e6c425c8d9ae54c3cc2c53d3677e8feb917f7e9fbc91646ec0e69dfba3</citedby><cites>FETCH-LOGICAL-c389t-8012716e6c425c8d9ae54c3cc2c53d3677e8feb917f7e9fbc91646ec0e69dfba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(16)54972-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4349455$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1313377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohashi, Kazutomo</creatorcontrib><creatorcontrib>Saji, Fumitaka</creatorcontrib><creatorcontrib>Wakimoto, Akinori</creatorcontrib><creatorcontrib>Kato, Munehiro</creatorcontrib><creatorcontrib>Tsutsui, Tateki</creatorcontrib><creatorcontrib>Tanizawa, Osamu</creatorcontrib><title>Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column.
The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count<20×106/mL, sperm motility <40%). After concentration of motile sperm using a 40% Percoll density gradient centrifugation, the sperm suspension was filtered through the SpermPrep column. The percentage yield of motile sperm by the SpermPrep method was compared with those by a two-layer Percoll density gradient (Pharmacia, Uppsala, Sweden) centrifugation and a swim-up method. Infertile couples with poor quality semen were treated with intrauterine insemination (IUI) with motile sperm by the three preparations through three cycles.
Department of Obstetrics and Gynecology, Osaka University Hospital.
Twenty-one couples with long-standing infertility because of poor quality semen.
Recovery of motile sperm, sperm motility, and outcome of IUI were evaluated among three semen preparations.
Motility was improved by the SpermPrep method in 32 of 33 cases of oligozoospermia and/or asthenozoospermia. Percentage yield of motile sperm by the SpermPrep method was significantly greater than those by the two-layer Percoll density gradient and swim-up methods (42.7±4.6 versus 22.1±3.1 and 13.8±3.5), but there is no significant difference in the sperm motility among three semen preparations. After one treatment cycle for each preparation, 2 of 21 women conceived after IUI with motile sperm separated in the SpermPrep method.
The SpermPrep method is an improved semen manipulation method for oligozoospermia and/or asthenozoospermia.</description><subject>asthenozoospermia</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cell Separation</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Disposable Equipment</subject><subject>Female</subject><subject>Filtration - instrumentation</subject><subject>Filtration - methods</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Insemination, Artificial, Homologous - instrumentation</subject><subject>Insemination, Artificial, Homologous - methods</subject><subject>intrauterine insemination</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Oligospermia - physiopathology</subject><subject>oligozoospermia</subject><subject>Percoll density gradient centrifugation</subject><subject>Povidone</subject><subject>Semen</subject><subject>Silicon Dioxide</subject><subject>Sperm Motility</subject><subject>Spermatozoa - cytology</subject><subject>SpermPrep semen filtration column</subject><subject>Sterility. Assisted procreation</subject><subject>swim-up method</subject><subject>Uterus</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcFu1TAQtBCoPAqfUCmHCsEh1I5jOz6hqgJaqRJIhbPlt1kXo8R-tZNK7Q_0t3GaR-HGabWamZ3VDCFHjH5glMmTK0qZqGnTNe-YfC9arZqaPyMbJoSshRT8Odk8UV6SVzn_opRKppoDcsA441ypDXn4lnBnk518DFV0VRz8dbyPMe8wjR4qG_qTmCqbp58Y_gUyjhgqVzAfpmTnCZMPWJYC-LDem7MP11VRVleLarH6o_PDtDeFOMxjeE1eODtkfLOfh-TH50_fz87ry69fLs5OL2vgnZ7qjrJGMYkS2kZA12uLogUO0IDgPZdKYedwq5lyCrXbgmaylQgUpe7d1vJD8na9u0vxZsY8mdFnwGGwAeOcjWo6KbkWhShWIqSYc0JndsmPNt0ZRs1SgHkswCzpmrI9FmB40R3tDebtiP1f1Zp4wY_3uM1gB5dsAJ-faC1vdSsW-48rDUsYtx6TyeAxAPY-IUymj_4_j_wGnCemyA</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>Ohashi, Kazutomo</creator><creator>Saji, Fumitaka</creator><creator>Wakimoto, Akinori</creator><creator>Kato, Munehiro</creator><creator>Tsutsui, Tateki</creator><creator>Tanizawa, Osamu</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920401</creationdate><title>Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column</title><author>Ohashi, Kazutomo ; Saji, Fumitaka ; Wakimoto, Akinori ; Kato, Munehiro ; Tsutsui, Tateki ; Tanizawa, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-8012716e6c425c8d9ae54c3cc2c53d3677e8feb917f7e9fbc91646ec0e69dfba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>asthenozoospermia</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cell Separation</topic><topic>Centrifugation, Density Gradient - methods</topic><topic>Disposable Equipment</topic><topic>Female</topic><topic>Filtration - instrumentation</topic><topic>Filtration - methods</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Insemination, Artificial, Homologous - instrumentation</topic><topic>Insemination, Artificial, Homologous - methods</topic><topic>intrauterine insemination</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Oligospermia - physiopathology</topic><topic>oligozoospermia</topic><topic>Percoll density gradient centrifugation</topic><topic>Povidone</topic><topic>Semen</topic><topic>Silicon Dioxide</topic><topic>Sperm Motility</topic><topic>Spermatozoa - cytology</topic><topic>SpermPrep semen filtration column</topic><topic>Sterility. Assisted procreation</topic><topic>swim-up method</topic><topic>Uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohashi, Kazutomo</creatorcontrib><creatorcontrib>Saji, Fumitaka</creatorcontrib><creatorcontrib>Wakimoto, Akinori</creatorcontrib><creatorcontrib>Kato, Munehiro</creatorcontrib><creatorcontrib>Tsutsui, Tateki</creatorcontrib><creatorcontrib>Tanizawa, Osamu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohashi, Kazutomo</au><au>Saji, Fumitaka</au><au>Wakimoto, Akinori</au><au>Kato, Munehiro</au><au>Tsutsui, Tateki</au><au>Tanizawa, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>57</volume><issue>4</issue><spage>866</spage><epage>870</epage><pages>866-870</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column.
The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count<20×106/mL, sperm motility <40%). After concentration of motile sperm using a 40% Percoll density gradient centrifugation, the sperm suspension was filtered through the SpermPrep column. The percentage yield of motile sperm by the SpermPrep method was compared with those by a two-layer Percoll density gradient (Pharmacia, Uppsala, Sweden) centrifugation and a swim-up method. Infertile couples with poor quality semen were treated with intrauterine insemination (IUI) with motile sperm by the three preparations through three cycles.
Department of Obstetrics and Gynecology, Osaka University Hospital.
Twenty-one couples with long-standing infertility because of poor quality semen.
Recovery of motile sperm, sperm motility, and outcome of IUI were evaluated among three semen preparations.
Motility was improved by the SpermPrep method in 32 of 33 cases of oligozoospermia and/or asthenozoospermia. Percentage yield of motile sperm by the SpermPrep method was significantly greater than those by the two-layer Percoll density gradient and swim-up methods (42.7±4.6 versus 22.1±3.1 and 13.8±3.5), but there is no significant difference in the sperm motility among three semen preparations. After one treatment cycle for each preparation, 2 of 21 women conceived after IUI with motile sperm separated in the SpermPrep method.
The SpermPrep method is an improved semen manipulation method for oligozoospermia and/or asthenozoospermia.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>1313377</pmid><doi>10.1016/S0015-0282(16)54972-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-0282 |
| ispartof | Fertility and sterility, 1992-04, Vol.57 (4), p.866-870 |
| issn | 0015-0282 1556-5653 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72866395 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | asthenozoospermia Biological and medical sciences Birth control Cell Separation Centrifugation, Density Gradient - methods Disposable Equipment Female Filtration - instrumentation Filtration - methods Gynecology. Andrology. Obstetrics Humans Insemination, Artificial, Homologous - instrumentation Insemination, Artificial, Homologous - methods intrauterine insemination Male Medical sciences Oligospermia - physiopathology oligozoospermia Percoll density gradient centrifugation Povidone Semen Silicon Dioxide Sperm Motility Spermatozoa - cytology SpermPrep semen filtration column Sterility. Assisted procreation swim-up method Uterus |
| title | Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column |
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