Sphingolipid transport from the trans-Golgi network to the apical surface in permeabilized MDCK cells
We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans-Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin-Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dep...
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| Veröffentlicht in: | FEBS letters 1992-04, Vol.300 (3), p.227-231 |
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| creator | Kobayashi, Toshihide Pimplikar, Sanjay W. Parton, Robert G. Bhakdi, Sucharit Simons, Kai |
| description | We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans-Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin-Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN-derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic. |
| doi_str_mv | 10.1016/0014-5793(92)80851-7 |
| format | Article |
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Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN-derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(92)80851-7</identifier><identifier>PMID: 1555649</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Apical membrane ; Biological and medical sciences ; Biological Transport ; Bovine serum albumin ; BSA ; C6-NBD-Cer ; C6-NBD-GleCer ; C6-NBD-SM ; Cell Line ; Cell Membrane Permeability ; Cell physiology ; Dogs ; epithelium ; Fluorescent lipid analog ; Fundamental and applied biological sciences. Psychology ; Glucosylceramides - metabolism ; Golgi apparatus ; Golgi Apparatus - drug effects ; Golgi Apparatus - metabolism ; Golgi Apparatus - ultrastructure ; Hemagglutinin ; kidney ; Kidney - metabolism ; Kidney - ultrastructure ; Lactate dehydrogenase ; LDH ; Madin Darby canine kidney ; MDCK ; MDCK cell ; MDCK cells ; Membrane and intracellular transports ; Membrane Lipids - metabolism ; Molecular and cellular biology ; N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl d-erythro-sphingosine ; N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine glucoside ; N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine-1-phosphocholine ; Permeabilized cell ; Serum Albumin, Bovine - pharmacology ; SLO ; Sphingolipid transport ; sphingolipids ; Sphingolipids - metabolism ; Sphingomyelins - metabolism ; Streptolysin O ; TGN ; Trans Golgi network ; transport</subject><ispartof>FEBS letters, 1992-04, Vol.300 (3), p.227-231</ispartof><rights>1992</rights><rights>FEBS Letters 300 (1992) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4617-2de4955b6878ae07c523ab6d67140f34095623e40b24ab8a9698001422555d63</citedby><cites>FETCH-LOGICAL-c4617-2de4955b6878ae07c523ab6d67140f34095623e40b24ab8a9698001422555d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(92)80851-7$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5153703$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1555649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobayashi, Toshihide</creatorcontrib><creatorcontrib>Pimplikar, Sanjay W.</creatorcontrib><creatorcontrib>Parton, Robert G.</creatorcontrib><creatorcontrib>Bhakdi, Sucharit</creatorcontrib><creatorcontrib>Simons, Kai</creatorcontrib><title>Sphingolipid transport from the trans-Golgi network to the apical surface in permeabilized MDCK cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans-Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin-Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN-derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic.</description><subject>Animals</subject><subject>Apical membrane</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Bovine serum albumin</subject><subject>BSA</subject><subject>C6-NBD-Cer</subject><subject>C6-NBD-GleCer</subject><subject>C6-NBD-SM</subject><subject>Cell Line</subject><subject>Cell Membrane Permeability</subject><subject>Cell physiology</subject><subject>Dogs</subject><subject>epithelium</subject><subject>Fluorescent lipid analog</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucosylceramides - metabolism</subject><subject>Golgi apparatus</subject><subject>Golgi Apparatus - drug effects</subject><subject>Golgi Apparatus - metabolism</subject><subject>Golgi Apparatus - ultrastructure</subject><subject>Hemagglutinin</subject><subject>kidney</subject><subject>Kidney - metabolism</subject><subject>Kidney - ultrastructure</subject><subject>Lactate dehydrogenase</subject><subject>LDH</subject><subject>Madin Darby canine kidney</subject><subject>MDCK</subject><subject>MDCK cell</subject><subject>MDCK cells</subject><subject>Membrane and intracellular transports</subject><subject>Membrane Lipids - metabolism</subject><subject>Molecular and cellular biology</subject><subject>N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl d-erythro-sphingosine</subject><subject>N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine glucoside</subject><subject>N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine-1-phosphocholine</subject><subject>Permeabilized cell</subject><subject>Serum Albumin, Bovine - pharmacology</subject><subject>SLO</subject><subject>Sphingolipid transport</subject><subject>sphingolipids</subject><subject>Sphingolipids - metabolism</subject><subject>Sphingomyelins - metabolism</subject><subject>Streptolysin O</subject><subject>TGN</subject><subject>Trans Golgi network</subject><subject>transport</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1v1DAUtBBVWQr_ACQfEGoPAdvx5wUJlm5BtOqB3i3HeWkNSZza2Vbl15NsVuUGnCzPzPuYeQi9ouQdJVS-J4TyQihTHht2ookWtFBP0IpqVRYll_opWj1KnqHnOf8g019Tc4gOqRBCcrNC8H24Cf11bMMQajwm1-chphE3KXZ4vIEFKs5iex1wD-N9TD_xGHeUG4J3Lc7b1DgPOPR4gNSBq0IbfkGNLz6vv2EPbZtfoIPGtRle7t8jdLU5vVp_Kc4vz76uP54XnkuqClYDN0JUUivtgCgvWOkqWUtFOWlKToyQrAROKsZdpZ2RRs8WGZvs1LI8Qm-XtkOKt1vIo-1CnhdwPcRttoppySUp_ymkkhM6DZ2EfBH6FHNO0Nghhc6lB0uJna9g5_l2jtgaZndXsGoqe73vv606qP8ULbFP_Js97_IUYTNl7EN-lAkqSrVbc7PI7kMLD_812m5OP7GZmHHDdui8z4elEUzh3wVINvsAvYc6JPCjrWP4u6HfDHW1BA</recordid><startdate>19920406</startdate><enddate>19920406</enddate><creator>Kobayashi, Toshihide</creator><creator>Pimplikar, Sanjay W.</creator><creator>Parton, Robert G.</creator><creator>Bhakdi, Sucharit</creator><creator>Simons, Kai</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920406</creationdate><title>Sphingolipid transport from the trans-Golgi network to the apical surface in permeabilized MDCK cells</title><author>Kobayashi, Toshihide ; Pimplikar, Sanjay W. ; Parton, Robert G. ; Bhakdi, Sucharit ; Simons, Kai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4617-2de4955b6878ae07c523ab6d67140f34095623e40b24ab8a9698001422555d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Apical membrane</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Bovine serum albumin</topic><topic>BSA</topic><topic>C6-NBD-Cer</topic><topic>C6-NBD-GleCer</topic><topic>C6-NBD-SM</topic><topic>Cell Line</topic><topic>Cell Membrane Permeability</topic><topic>Cell physiology</topic><topic>Dogs</topic><topic>epithelium</topic><topic>Fluorescent lipid analog</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucosylceramides - metabolism</topic><topic>Golgi apparatus</topic><topic>Golgi Apparatus - drug effects</topic><topic>Golgi Apparatus - metabolism</topic><topic>Golgi Apparatus - ultrastructure</topic><topic>Hemagglutinin</topic><topic>kidney</topic><topic>Kidney - metabolism</topic><topic>Kidney - ultrastructure</topic><topic>Lactate dehydrogenase</topic><topic>LDH</topic><topic>Madin Darby canine kidney</topic><topic>MDCK</topic><topic>MDCK cell</topic><topic>MDCK cells</topic><topic>Membrane and intracellular transports</topic><topic>Membrane Lipids - metabolism</topic><topic>Molecular and cellular biology</topic><topic>N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl d-erythro-sphingosine</topic><topic>N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine glucoside</topic><topic>N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine-1-phosphocholine</topic><topic>Permeabilized cell</topic><topic>Serum Albumin, Bovine - pharmacology</topic><topic>SLO</topic><topic>Sphingolipid transport</topic><topic>sphingolipids</topic><topic>Sphingolipids - metabolism</topic><topic>Sphingomyelins - metabolism</topic><topic>Streptolysin O</topic><topic>TGN</topic><topic>Trans Golgi network</topic><topic>transport</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Toshihide</creatorcontrib><creatorcontrib>Pimplikar, Sanjay W.</creatorcontrib><creatorcontrib>Parton, Robert G.</creatorcontrib><creatorcontrib>Bhakdi, Sucharit</creatorcontrib><creatorcontrib>Simons, Kai</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Toshihide</au><au>Pimplikar, Sanjay W.</au><au>Parton, Robert G.</au><au>Bhakdi, Sucharit</au><au>Simons, Kai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sphingolipid transport from the trans-Golgi network to the apical surface in permeabilized MDCK cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1992-04-06</date><risdate>1992</risdate><volume>300</volume><issue>3</issue><spage>227</spage><epage>231</epage><pages>227-231</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans-Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin-Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN-derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1555649</pmid><doi>10.1016/0014-5793(92)80851-7</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1992-04, Vol.300 (3), p.227-231 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72864603 |
| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Apical membrane Biological and medical sciences Biological Transport Bovine serum albumin BSA C6-NBD-Cer C6-NBD-GleCer C6-NBD-SM Cell Line Cell Membrane Permeability Cell physiology Dogs epithelium Fluorescent lipid analog Fundamental and applied biological sciences. Psychology Glucosylceramides - metabolism Golgi apparatus Golgi Apparatus - drug effects Golgi Apparatus - metabolism Golgi Apparatus - ultrastructure Hemagglutinin kidney Kidney - metabolism Kidney - ultrastructure Lactate dehydrogenase LDH Madin Darby canine kidney MDCK MDCK cell MDCK cells Membrane and intracellular transports Membrane Lipids - metabolism Molecular and cellular biology N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl d-erythro-sphingosine N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine glucoside N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-6-aminocaproyl sphingosine-1-phosphocholine Permeabilized cell Serum Albumin, Bovine - pharmacology SLO Sphingolipid transport sphingolipids Sphingolipids - metabolism Sphingomyelins - metabolism Streptolysin O TGN Trans Golgi network transport |
| title | Sphingolipid transport from the trans-Golgi network to the apical surface in permeabilized MDCK cells |
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