Ceramide increases Fas-mediated apoptosis in glioblastoma cells through FLIP down-regulation
Ceramide is a physiologic regulator of growth and differentiation in mammalian cells. In this study, the relationship between ceramide and FLICE inhibitory protein (FLIP) in the induction of apoptosis in glioblastoma cell lines was investigated. We found that LN215 cells were slightly more sensitive...
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| Veröffentlicht in: | Journal of neuro-oncology 2002-11, Vol.60 (2), p.135-141 |
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| container_title | Journal of neuro-oncology |
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| creator | YOON, Gitae KIM, Kyoung-Ok LEE, Jeonggi KWON, Daeho SHIN, Jeon-Soo KIM, Se-Jong CHOI, In-Hong |
| description | Ceramide is a physiologic regulator of growth and differentiation in mammalian cells. In this study, the relationship between ceramide and FLICE inhibitory protein (FLIP) in the induction of apoptosis in glioblastoma cell lines was investigated. We found that LN215 cells were slightly more sensitive to Fas-mediated apoptosis than LN319 cells, which were more sensitive to ceramide than LN215 cells. FLIP was expressed in LN319 and LN215 cells constitutively, and this expression decreased with treatment of ceramide in LN215 cells, which might cause LN215 cells to be more sensitive to Fas-mediated apoptosis at lower level stimulation. In LN319 cells FLIP levels were not modified by ceramide treatment and the level of cell death induced by anti-Fas antibody was not affected. Our results suggest that FLIP may be down-regulated by low levels of ceramide in LN215 cells, which causes LN215 cells to be more sensitive to Fas-mediated apoptosis, whereas LN319 cells remain resistant. |
| doi_str_mv | 10.1023/A:1020604705831 |
| format | Article |
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In this study, the relationship between ceramide and FLICE inhibitory protein (FLIP) in the induction of apoptosis in glioblastoma cell lines was investigated. We found that LN215 cells were slightly more sensitive to Fas-mediated apoptosis than LN319 cells, which were more sensitive to ceramide than LN215 cells. FLIP was expressed in LN319 and LN215 cells constitutively, and this expression decreased with treatment of ceramide in LN215 cells, which might cause LN215 cells to be more sensitive to Fas-mediated apoptosis at lower level stimulation. In LN319 cells FLIP levels were not modified by ceramide treatment and the level of cell death induced by anti-Fas antibody was not affected. Our results suggest that FLIP may be down-regulated by low levels of ceramide in LN215 cells, which causes LN215 cells to be more sensitive to Fas-mediated apoptosis, whereas LN319 cells remain resistant.</description><identifier>ISSN: 0167-594X</identifier><identifier>EISSN: 1573-7373</identifier><identifier>DOI: 10.1023/A:1020604705831</identifier><identifier>PMID: 12635660</identifier><identifier>CODEN: JNODD2</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Apoptosis - drug effects ; bcl-2-Associated X Protein ; Biological and medical sciences ; Carrier Proteins - metabolism ; CASP8 and FADD-Like Apoptosis Regulating Protein ; Ceramides - pharmacology ; Down-Regulation - drug effects ; fas Receptor - metabolism ; Flow Cytometry ; Glioblastoma ; Humans ; Intracellular Signaling Peptides and Proteins ; Medical sciences ; Neurology ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-bcl-2 ; Tumor Cells, Cultured - cytology ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism ; Tumors of the nervous system. Phacomatoses</subject><ispartof>Journal of neuro-oncology, 2002-11, Vol.60 (2), p.135-141</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers Nov 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c310t-96349d7f203bd41a73f60353c5ba2e05eec3effc480f284b84dea9ea11632d723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13997794$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12635660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOON, Gitae</creatorcontrib><creatorcontrib>KIM, Kyoung-Ok</creatorcontrib><creatorcontrib>LEE, Jeonggi</creatorcontrib><creatorcontrib>KWON, Daeho</creatorcontrib><creatorcontrib>SHIN, Jeon-Soo</creatorcontrib><creatorcontrib>KIM, Se-Jong</creatorcontrib><creatorcontrib>CHOI, In-Hong</creatorcontrib><title>Ceramide increases Fas-mediated apoptosis in glioblastoma cells through FLIP down-regulation</title><title>Journal of neuro-oncology</title><addtitle>J Neurooncol</addtitle><description>Ceramide is a physiologic regulator of growth and differentiation in mammalian cells. In this study, the relationship between ceramide and FLICE inhibitory protein (FLIP) in the induction of apoptosis in glioblastoma cell lines was investigated. We found that LN215 cells were slightly more sensitive to Fas-mediated apoptosis than LN319 cells, which were more sensitive to ceramide than LN215 cells. FLIP was expressed in LN319 and LN215 cells constitutively, and this expression decreased with treatment of ceramide in LN215 cells, which might cause LN215 cells to be more sensitive to Fas-mediated apoptosis at lower level stimulation. In LN319 cells FLIP levels were not modified by ceramide treatment and the level of cell death induced by anti-Fas antibody was not affected. Our results suggest that FLIP may be down-regulated by low levels of ceramide in LN215 cells, which causes LN215 cells to be more sensitive to Fas-mediated apoptosis, whereas LN319 cells remain resistant.</description><subject>Apoptosis - drug effects</subject><subject>bcl-2-Associated X Protein</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - metabolism</subject><subject>CASP8 and FADD-Like Apoptosis Regulating Protein</subject><subject>Ceramides - pharmacology</subject><subject>Down-Regulation - drug effects</subject><subject>fas Receptor - metabolism</subject><subject>Flow Cytometry</subject><subject>Glioblastoma</subject><subject>Humans</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Medical sciences</subject><subject>Neurology</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2</subject><subject>Tumor Cells, Cultured - cytology</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Tumors of the nervous system. 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Phacomatoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOON, Gitae</creatorcontrib><creatorcontrib>KIM, Kyoung-Ok</creatorcontrib><creatorcontrib>LEE, Jeonggi</creatorcontrib><creatorcontrib>KWON, Daeho</creatorcontrib><creatorcontrib>SHIN, Jeon-Soo</creatorcontrib><creatorcontrib>KIM, Se-Jong</creatorcontrib><creatorcontrib>CHOI, In-Hong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuro-oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOON, Gitae</au><au>KIM, Kyoung-Ok</au><au>LEE, Jeonggi</au><au>KWON, Daeho</au><au>SHIN, Jeon-Soo</au><au>KIM, Se-Jong</au><au>CHOI, In-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ceramide increases Fas-mediated apoptosis in glioblastoma cells through FLIP down-regulation</atitle><jtitle>Journal of neuro-oncology</jtitle><addtitle>J Neurooncol</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>60</volume><issue>2</issue><spage>135</spage><epage>141</epage><pages>135-141</pages><issn>0167-594X</issn><eissn>1573-7373</eissn><coden>JNODD2</coden><abstract>Ceramide is a physiologic regulator of growth and differentiation in mammalian cells. In this study, the relationship between ceramide and FLICE inhibitory protein (FLIP) in the induction of apoptosis in glioblastoma cell lines was investigated. We found that LN215 cells were slightly more sensitive to Fas-mediated apoptosis than LN319 cells, which were more sensitive to ceramide than LN215 cells. FLIP was expressed in LN319 and LN215 cells constitutively, and this expression decreased with treatment of ceramide in LN215 cells, which might cause LN215 cells to be more sensitive to Fas-mediated apoptosis at lower level stimulation. In LN319 cells FLIP levels were not modified by ceramide treatment and the level of cell death induced by anti-Fas antibody was not affected. Our results suggest that FLIP may be down-regulated by low levels of ceramide in LN215 cells, which causes LN215 cells to be more sensitive to Fas-mediated apoptosis, whereas LN319 cells remain resistant.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>12635660</pmid><doi>10.1023/A:1020604705831</doi><tpages>7</tpages></addata></record> |
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| subjects | Apoptosis - drug effects bcl-2-Associated X Protein Biological and medical sciences Carrier Proteins - metabolism CASP8 and FADD-Like Apoptosis Regulating Protein Ceramides - pharmacology Down-Regulation - drug effects fas Receptor - metabolism Flow Cytometry Glioblastoma Humans Intracellular Signaling Peptides and Proteins Medical sciences Neurology Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-bcl-2 Tumor Cells, Cultured - cytology Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism Tumors of the nervous system. Phacomatoses |
| title | Ceramide increases Fas-mediated apoptosis in glioblastoma cells through FLIP down-regulation |
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