A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling
A large gold cluster (Au1.4nm) was covalently coupled to IgG and Fab' fragments. Its gold core is 1.4 nm in diameter and the Fab'-Au1.4nm immunoconjugate is the smallest gold immunoprobe that can be seen directly in the conventional electron microscope. It is useful in high-resolution immu...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1992-02, Vol.40 (2), p.177-184 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Hainfeld, JF Furuya, FR |
| description | A large gold cluster (Au1.4nm) was covalently coupled to IgG and Fab' fragments. Its gold core is 1.4 nm in diameter and the Fab'-Au1.4nm immunoconjugate is the smallest gold immunoprobe that can be seen directly in the conventional electron microscope. It is useful in high-resolution immunolabeling, providing a resolution of 7.0 nm. The cluster's visibility can be enhanced with silver development for use in EM or light microscopy for histological purposes, or to detect less than or equal to 0.2 pg of antigen in immunoblots. By using a gold compound with covalent attachment, a number of advantages over colloidal gold probes are realized, including better resolution, stability, uniformity, sensitivity, and complete absence of aggregation; its small size should also improve penetration and more quantitative labeling of antigenic sites. |
| doi_str_mv | 10.1177/40.2.1552162 |
| format | Article |
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Psychology ; Fundamental immunology ; Gold ; HeLa Cells ; Humans ; Immunoglobulin Fab Fragments ; Immunoglobulin G ; Immunohistochemistry - methods ; Microscopy, Electron, Scanning - methods ; Microscopy, Immunoelectron - methods ; Molecular immunology ; Rabbits - immunology ; Techniques</subject><ispartof>The journal of histochemistry and cytochemistry, 1992-02, Vol.40 (2), p.177-184</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-e3ba1cf08a0808686944b02f2998f85e7ad161ec78a03586d3ef70e9da15571a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/40.2.1552162$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/40.2.1552162$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21810,27915,27916,43612,43613</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5576482$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1552162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hainfeld, JF</creatorcontrib><creatorcontrib>Furuya, FR</creatorcontrib><title>A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>A large gold cluster (Au1.4nm) was covalently coupled to IgG and Fab' fragments. Its gold core is 1.4 nm in diameter and the Fab'-Au1.4nm immunoconjugate is the smallest gold immunoprobe that can be seen directly in the conventional electron microscope. It is useful in high-resolution immunolabeling, providing a resolution of 7.0 nm. The cluster's visibility can be enhanced with silver development for use in EM or light microscopy for histological purposes, or to detect less than or equal to 0.2 pg of antigen in immunoblots. By using a gold compound with covalent attachment, a number of advantages over colloidal gold probes are realized, including better resolution, stability, uniformity, sensitivity, and complete absence of aggregation; its small size should also improve penetration and more quantitative labeling of antigenic sites.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Erythrocytes - immunology</subject><subject>Ferritins - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gold</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunoglobulin Fab Fragments</subject><subject>Immunoglobulin G</subject><subject>Immunohistochemistry - methods</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Microscopy, Immunoelectron - methods</subject><subject>Molecular immunology</subject><subject>Rabbits - immunology</subject><subject>Techniques</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFq3DAQBmARGrbbJLdeCz40hUK8kWTZ0h5DaNJAIJfkLMbyeFeLbKWSnSVvX21stqeeNGg-Zn6GkK-MrhiT8lrQFV-xsuSs4idkmSqWl1SIT2RJKed5-hCfyZcYd5QyIUq1IIuZL8nTTcZWIu-7bONdkxk3xgFDZvwbOOwH957BMIDZYpMNPoN-sLVvLMbMdq_Bv30U3dh7BzU622_OyWkLLuLF_J6Rl7tfz7e_88en-4fbm8fcCFUMORY1MNNSBVRRValqLURNecvXa9WqEiU0rGJoZAJFqaqmwFZSXDeQkksGxRn5Mc1NKf6MGAfd2WjQOejRj1FLripaFirBqwma4GMM2OrXYDsI75pRfbifFlRzPR8k8W_z3LHusPmHj_3vcx-iAdcG6I2NR5bCVUId2M-JRdig3vkx9Oka_1t5Odmt3Wz3NqCOHTiXAjC93-8nK2XxF4Qhj7Q</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>Hainfeld, JF</creator><creator>Furuya, FR</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920201</creationdate><title>A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling</title><author>Hainfeld, JF ; Furuya, FR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-e3ba1cf08a0808686944b02f2998f85e7ad161ec78a03586d3ef70e9da15571a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Erythrocytes - immunology</topic><topic>Ferritins - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gold</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunoglobulin Fab Fragments</topic><topic>Immunoglobulin G</topic><topic>Immunohistochemistry - methods</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Microscopy, Immunoelectron - methods</topic><topic>Molecular immunology</topic><topic>Rabbits - immunology</topic><topic>Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hainfeld, JF</creatorcontrib><creatorcontrib>Furuya, FR</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hainfeld, JF</au><au>Furuya, FR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>40</volume><issue>2</issue><spage>177</spage><epage>184</epage><pages>177-184</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>A large gold cluster (Au1.4nm) was covalently coupled to IgG and Fab' fragments. Its gold core is 1.4 nm in diameter and the Fab'-Au1.4nm immunoconjugate is the smallest gold immunoprobe that can be seen directly in the conventional electron microscope. It is useful in high-resolution immunolabeling, providing a resolution of 7.0 nm. The cluster's visibility can be enhanced with silver development for use in EM or light microscopy for histological purposes, or to detect less than or equal to 0.2 pg of antigen in immunoblots. By using a gold compound with covalent attachment, a number of advantages over colloidal gold probes are realized, including better resolution, stability, uniformity, sensitivity, and complete absence of aggregation; its small size should also improve penetration and more quantitative labeling of antigenic sites.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>1552162</pmid><doi>10.1177/40.2.1552162</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The journal of histochemistry and cytochemistry, 1992-02, Vol.40 (2), p.177-184 |
| issn | 0022-1554 1551-5044 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SAGE Complete A-Z List; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological and medical sciences Erythrocytes - immunology Ferritins - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology Gold HeLa Cells Humans Immunoglobulin Fab Fragments Immunoglobulin G Immunohistochemistry - methods Microscopy, Electron, Scanning - methods Microscopy, Immunoelectron - methods Molecular immunology Rabbits - immunology Techniques |
| title | A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling |
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