Yeast proteins associated with microtubules in vitro and in vivo

Conditions were established for the self-assembly of milligram amounts of purified Saccharomyces cerevisiae tubulin. Microtubules assembled with pure yeast tubulin were not stabilized by taxol; hybrid microtubules containing substoichiometric amounts of bovine tubulin were stabilized. Yeast microtub...

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Veröffentlicht in:	Molecular biology of the cell 1992-01, Vol.3 (1), p.29-47
Hauptverfasser:	Barnes, G, Louie, K A, Botstein, D
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaloids - pharmacology Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Fungal Proteins - chemistry Fungal Proteins - isolation & purification Fungal Proteins - metabolism GTP-Binding Proteins - chemistry GTP-Binding Proteins - isolation & purification GTP-Binding Proteins - metabolism Microtubule-Associated Proteins - chemistry Microtubule-Associated Proteins - isolation & purification Microtubule-Associated Proteins - metabolism Microtubules - chemistry Molecular Weight Paclitaxel Saccharomyces cerevisiae - chemistry Tubulin - chemistry Tubulin - isolation & purification
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