Retinyl palmitate hydrolase activity in human liver
Retinyl palmitate hydrolase (RPH) activity was studied in human liver after the optimal conditions of the assay in normal human liver homogenates were determined. The mean activity was 118 ± 66 nmol · min−1 · g−1 (x̄ ± SD) protein in liver homogenates from six children and seven adults; no correlati...
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| Veröffentlicht in: | The American journal of clinical nutrition 1992-03, Vol.55 (3), p.729-733 |
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| creator | Mourey, MS Amédée-Manesme, O |
| description | Retinyl palmitate hydrolase (RPH) activity was studied in human liver after the optimal conditions of the assay in normal human liver homogenates were determined. The mean activity was 118 ± 66 nmol · min−1 · g−1 (x̄ ± SD) protein in liver homogenates from six children and seven adults; no correlation was found between liver hydrolase activity and the enzyme endogenous substrate, ie, liver vitamin A concentrations. RPH activity was also studied in a human model of vitamin A deficiency represented by 22 children with biliary atresia: 11 patients with vitamin A deficiency (liver vitamin A concentration < 70 nmol/g wet wt of liver) and 11 patients with normal vitamin A status after vitamin A treatment. The enzymatic assay had to be conducted after the removal of endogenous bile salts by cholestyramine because bile salts are accumulated in the liver of children with biliary atresia. No correlation was found between RPH activity and vitamin A status. |
| doi_str_mv | 10.1093/ajcn/55.3.729 |
| format | Article |
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The mean activity was 118 ± 66 nmol · min−1 · g−1 (x̄ ± SD) protein in liver homogenates from six children and seven adults; no correlation was found between liver hydrolase activity and the enzyme endogenous substrate, ie, liver vitamin A concentrations. RPH activity was also studied in a human model of vitamin A deficiency represented by 22 children with biliary atresia: 11 patients with vitamin A deficiency (liver vitamin A concentration < 70 nmol/g wet wt of liver) and 11 patients with normal vitamin A status after vitamin A treatment. The enzymatic assay had to be conducted after the removal of endogenous bile salts by cholestyramine because bile salts are accumulated in the liver of children with biliary atresia. No correlation was found between RPH activity and vitamin A status.</description><identifier>ISSN: 0002-9165</identifier><identifier>EISSN: 1938-3207</identifier><identifier>DOI: 10.1093/ajcn/55.3.729</identifier><identifier>PMID: 1550049</identifier><identifier>CODEN: AJCNAC</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Biliary atresia ; Biliary Atresia - enzymology ; Biological and medical sciences ; Carboxylic Ester Hydrolases - metabolism ; Child ; Child, Preschool ; cholestyramine ; Cholic Acid ; Cholic Acids - pharmacology ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Kinetics ; Liver - enzymology ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Malformations ; Medical sciences ; regulation ; retinyl palmitate hydrolase ; vitamin A ; Vitamin A - metabolism ; Vitamin A Deficiency - enzymology</subject><ispartof>The American journal of clinical nutrition, 1992-03, Vol.55 (3), p.729-733</ispartof><rights>1992 American Society for Nutrition.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-d5df5c30407ba42e1e50144281b16e506871c1e7925e68d93afe9e609b02d4193</citedby><cites>FETCH-LOGICAL-c404t-d5df5c30407ba42e1e50144281b16e506871c1e7925e68d93afe9e609b02d4193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5222996$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1550049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mourey, MS</creatorcontrib><creatorcontrib>Amédée-Manesme, O</creatorcontrib><title>Retinyl palmitate hydrolase activity in human liver</title><title>The American journal of clinical nutrition</title><addtitle>Am J Clin Nutr</addtitle><description>Retinyl palmitate hydrolase (RPH) activity was studied in human liver after the optimal conditions of the assay in normal human liver homogenates were determined. The mean activity was 118 ± 66 nmol · min−1 · g−1 (x̄ ± SD) protein in liver homogenates from six children and seven adults; no correlation was found between liver hydrolase activity and the enzyme endogenous substrate, ie, liver vitamin A concentrations. RPH activity was also studied in a human model of vitamin A deficiency represented by 22 children with biliary atresia: 11 patients with vitamin A deficiency (liver vitamin A concentration < 70 nmol/g wet wt of liver) and 11 patients with normal vitamin A status after vitamin A treatment. The enzymatic assay had to be conducted after the removal of endogenous bile salts by cholestyramine because bile salts are accumulated in the liver of children with biliary atresia. No correlation was found between RPH activity and vitamin A status.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biliary atresia</subject><subject>Biliary Atresia - enzymology</subject><subject>Biological and medical sciences</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>cholestyramine</subject><subject>Cholic Acid</subject><subject>Cholic Acids - pharmacology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Liver - enzymology</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Malformations</subject><subject>Medical sciences</subject><subject>regulation</subject><subject>retinyl palmitate hydrolase</subject><subject>vitamin A</subject><subject>Vitamin A - metabolism</subject><subject>Vitamin A Deficiency - enzymology</subject><issn>0002-9165</issn><issn>1938-3207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LxDAQhoMo67p69Cj0IN66O0mTpjnK4hcsCKLnkKZTNks_1qRd6L-3Sxc9eZqBeeZl5iHklsKSgkpWZmeblRDLZCmZOiNzqpIsThjIczIHABYrmopLchXCDoAynqUzMqNCAHA1J8kHdq4Zqmhvqtp1psNoOxS-rUzAyNjOHVw3RK6Jtn1tmqhyB_TX5KI0VcCbU12Qr-enz_VrvHl_eVs_bmLLgXdxIYpS2AQ4yNxwhhQFUM5ZRnOajn2aSWopSsUEplmhElOiwhRUDqzg4xsL8jDl7n373WPodO2CxaoyDbZ90JJlIpUyG8F4Aq1vQ_BY6r13tfGDpqCPkvRRkhZCJ-PSMfjuFNznNRZ_9GRlnN-f5iZYU5XeNNaFX0wwxpRKR0xOGI4SDg69DtZhY7FwHm2ni9b9c8AP_-KBUw</recordid><startdate>19920301</startdate><enddate>19920301</enddate><creator>Mourey, MS</creator><creator>Amédée-Manesme, O</creator><general>Elsevier Inc</general><general>American Society for Clinical Nutrition</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920301</creationdate><title>Retinyl palmitate hydrolase activity in human liver</title><author>Mourey, MS ; Amédée-Manesme, O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-d5df5c30407ba42e1e50144281b16e506871c1e7925e68d93afe9e609b02d4193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biliary atresia</topic><topic>Biliary Atresia - enzymology</topic><topic>Biological and medical sciences</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>cholestyramine</topic><topic>Cholic Acid</topic><topic>Cholic Acids - pharmacology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Liver - enzymology</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Malformations</topic><topic>Medical sciences</topic><topic>regulation</topic><topic>retinyl palmitate hydrolase</topic><topic>vitamin A</topic><topic>Vitamin A - metabolism</topic><topic>Vitamin A Deficiency - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mourey, MS</creatorcontrib><creatorcontrib>Amédée-Manesme, O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of clinical nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mourey, MS</au><au>Amédée-Manesme, O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retinyl palmitate hydrolase activity in human liver</atitle><jtitle>The American journal of clinical nutrition</jtitle><addtitle>Am J Clin Nutr</addtitle><date>1992-03-01</date><risdate>1992</risdate><volume>55</volume><issue>3</issue><spage>729</spage><epage>733</epage><pages>729-733</pages><issn>0002-9165</issn><eissn>1938-3207</eissn><coden>AJCNAC</coden><abstract>Retinyl palmitate hydrolase (RPH) activity was studied in human liver after the optimal conditions of the assay in normal human liver homogenates were determined. The mean activity was 118 ± 66 nmol · min−1 · g−1 (x̄ ± SD) protein in liver homogenates from six children and seven adults; no correlation was found between liver hydrolase activity and the enzyme endogenous substrate, ie, liver vitamin A concentrations. RPH activity was also studied in a human model of vitamin A deficiency represented by 22 children with biliary atresia: 11 patients with vitamin A deficiency (liver vitamin A concentration < 70 nmol/g wet wt of liver) and 11 patients with normal vitamin A status after vitamin A treatment. The enzymatic assay had to be conducted after the removal of endogenous bile salts by cholestyramine because bile salts are accumulated in the liver of children with biliary atresia. No correlation was found between RPH activity and vitamin A status.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>1550049</pmid><doi>10.1093/ajcn/55.3.729</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0002-9165 1938-3207 |
| language | eng |
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| subjects | Adolescent Adult Biliary atresia Biliary Atresia - enzymology Biological and medical sciences Carboxylic Ester Hydrolases - metabolism Child Child, Preschool cholestyramine Cholic Acid Cholic Acids - pharmacology Gastroenterology. Liver. Pancreas. Abdomen Humans Kinetics Liver - enzymology Liver. Biliary tract. Portal circulation. Exocrine pancreas Malformations Medical sciences regulation retinyl palmitate hydrolase vitamin A Vitamin A - metabolism Vitamin A Deficiency - enzymology |
| title | Retinyl palmitate hydrolase activity in human liver |
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