Further molecular discrimination of Spanish strains of Echinococcus granulosus

We have designed two polymerase chain reaction (PCR) primer sets (PEg9F1-PEg9R1 and PEg16F1-PEg16R1) and two PCR protocols (Eg9-PCR and Eg16-PCR) for discrimination of Echinococcus granulosus genotypes. The oligonucleotide sequences originate from two E. granulosus DNA multiplex-PCR amplification fr...

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Veröffentlicht in:	Experimental parasitology 2002-09, Vol.102 (1), p.46-56
Hauptverfasser:	González, L.M., Daniel-Mwambete, K., Montero, E., Rosenzvit, M.C., McManus, D.P., Gárate, T., Cuesta-Bandera, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Blotting, Southern Cloning, Molecular cytochrome-c oxidase DNA, Helminth - chemistry DNA, Mitochondrial - chemistry echinococcosis Echinococcosis - parasitology Echinococcus - enzymology Echinococcus - genetics Echinococcus - isolation & purification Echinococcus granulosus Electron Transport Complex IV - genetics Fundamental and applied biological sciences. Psychology genes Genetic variation Genetics of eukaryotes. Biological and molecular evolution Genotype Horses Humans Invertebrata Mitochondrial DNA Molecular Sequence Data molecular systematics NADH dehydrogenase NADH Dehydrogenase - genetics ND1 gene nucleotide sequences Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Population genetics, reproduction patterns Restriction fragment length polymorphism Restriction Mapping Rodentia Sequence Alignment sequence analysis Sequence Analysis, DNA Sheep Spain Species Specificity strains Swine
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