Pancreatic antigenic complex p64 69: involvement in regulation of insulin secretion and relation to glutamic acid decarboxylase
The role of pancreatic beta-cell antigenic structures in modulation of insulin secretion in vitro was recently demonstrated by others. Here we report generation of a monoclonal antibody (mAB) ICA-1 to non-species specific beta-cell antigen(s) 64, 67 and 69 kDa. The mAB inhibits glucose stimulated in...
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| Veröffentlicht in: | Immunology letters 1992-02, Vol.31 (3), p.289-296 |
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| container_start_page | 289 |
| container_title | Immunology letters |
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| creator | Zlobina, Elena N. Dubinkin, Igor V. Merkushov, Alexei V. Volynskaya, Nadia A. Gudima, Georg O. |
| description | The role of pancreatic beta-cell antigenic structures in modulation of insulin secretion in vitro was recently demonstrated by others. Here we report generation of a monoclonal antibody (mAB) ICA-1 to non-species specific beta-cell antigen(s) 64, 67 and 69 kDa. The mAB inhibits glucose stimulated insulin secretion in islet cell cultures.
The ability of mABICA-1 to immunoprecipitate active glutamic acid decarboxylase from high speed supernatants of pancreatic and brain crude extracts was demonstrated. The 64, 67 and 69 kDa antigenic material was affinity purified from pancreatic islet cell high speed supernatants, active glutamic acid decarboxylase was found in the material.
Immunoaffinity purification with mAB ICA-1 of GAD-like pancreatic beta-cell antigenic material has provided evidence of possible involvement of glutamic acid decarboxylase in modulation of insulin secretion. |
| doi_str_mv | 10.1016/0165-2478(92)90129-C |
| format | Article |
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The ability of mABICA-1 to immunoprecipitate active glutamic acid decarboxylase from high speed supernatants of pancreatic and brain crude extracts was demonstrated. The 64, 67 and 69 kDa antigenic material was affinity purified from pancreatic islet cell high speed supernatants, active glutamic acid decarboxylase was found in the material.
Immunoaffinity purification with mAB ICA-1 of GAD-like pancreatic beta-cell antigenic material has provided evidence of possible involvement of glutamic acid decarboxylase in modulation of insulin secretion.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/0165-2478(92)90129-C</identifier><identifier>PMID: 1548042</identifier><identifier>CODEN: IMLED6</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antigenic determinants, haptens, artificial antigens ; Antigens ; Autoantigens - immunology ; Autoantigens - isolation & purification ; Biological and medical sciences ; Blotting, Western ; Cerebellum - enzymology ; Chromatography, Affinity ; Diabetes ; Electrophoresis, Polyacrylamide Gel ; Flow Cytometry ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; gamma-Aminobutyric Acid - metabolism ; Glutamate Decarboxylase - metabolism ; Glutamic acid decarboxylase ; Insulin - metabolism ; Insulin Secretion ; Islets of Langerhans - enzymology ; Islets of Langerhans - immunology ; Molecular immunology ; Molecular Weight ; Monoclonal antibody ; Rats</subject><ispartof>Immunology letters, 1992-02, Vol.31 (3), p.289-296</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-e6fe85d89fd91beca8d2ad3fb9eaac63e8d75b82468c3b83cc233a7200a7e2db3</citedby><cites>FETCH-LOGICAL-c386t-e6fe85d89fd91beca8d2ad3fb9eaac63e8d75b82468c3b83cc233a7200a7e2db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-2478(92)90129-C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5080080$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1548042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zlobina, Elena N.</creatorcontrib><creatorcontrib>Dubinkin, Igor V.</creatorcontrib><creatorcontrib>Merkushov, Alexei V.</creatorcontrib><creatorcontrib>Volynskaya, Nadia A.</creatorcontrib><creatorcontrib>Gudima, Georg O.</creatorcontrib><title>Pancreatic antigenic complex p64 69: involvement in regulation of insulin secretion and relation to glutamic acid decarboxylase</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>The role of pancreatic beta-cell antigenic structures in modulation of insulin secretion in vitro was recently demonstrated by others. Here we report generation of a monoclonal antibody (mAB) ICA-1 to non-species specific beta-cell antigen(s) 64, 67 and 69 kDa. The mAB inhibits glucose stimulated insulin secretion in islet cell cultures.
The ability of mABICA-1 to immunoprecipitate active glutamic acid decarboxylase from high speed supernatants of pancreatic and brain crude extracts was demonstrated. The 64, 67 and 69 kDa antigenic material was affinity purified from pancreatic islet cell high speed supernatants, active glutamic acid decarboxylase was found in the material.
Immunoaffinity purification with mAB ICA-1 of GAD-like pancreatic beta-cell antigenic material has provided evidence of possible involvement of glutamic acid decarboxylase in modulation of insulin secretion.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Autoantigens - immunology</subject><subject>Autoantigens - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cerebellum - enzymology</subject><subject>Chromatography, Affinity</subject><subject>Diabetes</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Glutamic acid decarboxylase</subject><subject>Insulin - metabolism</subject><subject>Insulin Secretion</subject><subject>Islets of Langerhans - enzymology</subject><subject>Islets of Langerhans - immunology</subject><subject>Molecular immunology</subject><subject>Molecular Weight</subject><subject>Monoclonal antibody</subject><subject>Rats</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtqHDEQRYVJsCeO_8CGXoSQLDrRox-SF4Ew5AUGe5GsRbVUPciopYnUPdir_Ho0nsHZBSRUlE5diUPIJaMfGGXdx7Lbmje9fKf4e0UZV_X6hKyY7FVN24a_IKtn5Iy8yvmeUtaKRpySU9Y2kjZ8Rf7cQTAJYXamgjC7DYZSmThtPT5U266pOnVdubCLfocThrnUVcLN4stIDFUcSyMvvnQzlqCnJgRbmCMxx2rjlxmm_QvG2cqigTTEh0cPGV-TlyP4jBfH85z8-vrl5_p7fXP77cf6801thOzmGrsRZWulGq1iQwmQloMV46AQwHQCpe3bQfKmk0YMUhjDhYCeUwo9cjuIc_L2kLtN8feCedaTywa9h4BxybrnshWUqQI2B9CkmHPCUW-TmyA9akb13rveS9V7qVpx_eRdr8vY1TF_GSa0_4YOosv9m-M9ZAN-TEW7y89YSyUtq2CfDhgWFzuHSWfjMBi0LqGZtY3u___4C1cboZ4</recordid><startdate>19920215</startdate><enddate>19920215</enddate><creator>Zlobina, Elena N.</creator><creator>Dubinkin, Igor V.</creator><creator>Merkushov, Alexei V.</creator><creator>Volynskaya, Nadia A.</creator><creator>Gudima, Georg O.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920215</creationdate><title>Pancreatic antigenic complex p64 69: involvement in regulation of insulin secretion and relation to glutamic acid decarboxylase</title><author>Zlobina, Elena N. ; Dubinkin, Igor V. ; Merkushov, Alexei V. ; Volynskaya, Nadia A. ; Gudima, Georg O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-e6fe85d89fd91beca8d2ad3fb9eaac63e8d75b82468c3b83cc233a7200a7e2db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Autoantigens - immunology</topic><topic>Autoantigens - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cerebellum - enzymology</topic><topic>Chromatography, Affinity</topic><topic>Diabetes</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Glutamic acid decarboxylase</topic><topic>Insulin - metabolism</topic><topic>Insulin Secretion</topic><topic>Islets of Langerhans - enzymology</topic><topic>Islets of Langerhans - immunology</topic><topic>Molecular immunology</topic><topic>Molecular Weight</topic><topic>Monoclonal antibody</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zlobina, Elena N.</creatorcontrib><creatorcontrib>Dubinkin, Igor V.</creatorcontrib><creatorcontrib>Merkushov, Alexei V.</creatorcontrib><creatorcontrib>Volynskaya, Nadia A.</creatorcontrib><creatorcontrib>Gudima, Georg O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zlobina, Elena N.</au><au>Dubinkin, Igor V.</au><au>Merkushov, Alexei V.</au><au>Volynskaya, Nadia A.</au><au>Gudima, Georg O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pancreatic antigenic complex p64 69: involvement in regulation of insulin secretion and relation to glutamic acid decarboxylase</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1992-02-15</date><risdate>1992</risdate><volume>31</volume><issue>3</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><coden>IMLED6</coden><abstract>The role of pancreatic beta-cell antigenic structures in modulation of insulin secretion in vitro was recently demonstrated by others. Here we report generation of a monoclonal antibody (mAB) ICA-1 to non-species specific beta-cell antigen(s) 64, 67 and 69 kDa. The mAB inhibits glucose stimulated insulin secretion in islet cell cultures.
The ability of mABICA-1 to immunoprecipitate active glutamic acid decarboxylase from high speed supernatants of pancreatic and brain crude extracts was demonstrated. The 64, 67 and 69 kDa antigenic material was affinity purified from pancreatic islet cell high speed supernatants, active glutamic acid decarboxylase was found in the material.
Immunoaffinity purification with mAB ICA-1 of GAD-like pancreatic beta-cell antigenic material has provided evidence of possible involvement of glutamic acid decarboxylase in modulation of insulin secretion.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1548042</pmid><doi>10.1016/0165-2478(92)90129-C</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| subjects | Animals Antibodies, Monoclonal - immunology Antigenic determinants, haptens, artificial antigens Antigens Autoantigens - immunology Autoantigens - isolation & purification Biological and medical sciences Blotting, Western Cerebellum - enzymology Chromatography, Affinity Diabetes Electrophoresis, Polyacrylamide Gel Flow Cytometry Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology gamma-Aminobutyric Acid - metabolism Glutamate Decarboxylase - metabolism Glutamic acid decarboxylase Insulin - metabolism Insulin Secretion Islets of Langerhans - enzymology Islets of Langerhans - immunology Molecular immunology Molecular Weight Monoclonal antibody Rats |
| title | Pancreatic antigenic complex p64 69: involvement in regulation of insulin secretion and relation to glutamic acid decarboxylase |
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