Degradation of cellular mRNA during influenza virus infection: its possible role in protein synthesis shutoff
1 Centro National de Biotecnología (CSIC), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid and 2 Centro de Biología Molecular (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain The kinetics of cellular mRNA decay in influenza virus-infected cells have been studied by means of blot hybridization...
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| Veröffentlicht in: | Journal of general virology 1992-03, Vol.73 (3), p.575-581 |
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| container_title | Journal of general virology |
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| creator | Beloso, Ana Martinez, Concepcion Valcarcel, Juan Santaren, Juan Fernandez Ortin, Juan |
| description | 1 Centro National de Biotecnología (CSIC), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid
and 2 Centro de Biología Molecular (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain
The kinetics of cellular mRNA decay in influenza virus-infected cells have been studied by means of blot hybridization using as probes cloned cDNAs of - and -actin, - and -tubulin and vimentin. Both cellular mRNAs isolated from the cytoplasmic fractions as well as total cell mRNAs showed a rapid decay, with up to 50% concentration reductions at infection times at which influenza virus M1 mRNA was still not detectable. In contrast, these cellular mRNAs were stable in uninfected cells. To ascertain the possible role of mRNA degradation in the cellular protein synthesis shutoff, the kinetics of protein synthesis in infected cells were examined by two-dimensional gel electrophoresis of extracts pulse-labelled at several times after viral infection. The synthesis of the cellular proteins was reduced, showing kinetics paralleling those of mRNA decay. It is proposed that influenza virus infection induces the destabilization of mRNAs and that this mRNA degradation is, at least in part, responsible for cellular protein synthesis shutoff.
Received 11 September 1991;
accepted 18 November 1991. |
| doi_str_mv | 10.1099/0022-1317-73-3-575 |
| format | Article |
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and 2 Centro de Biología Molecular (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain
The kinetics of cellular mRNA decay in influenza virus-infected cells have been studied by means of blot hybridization using as probes cloned cDNAs of - and -actin, - and -tubulin and vimentin. Both cellular mRNAs isolated from the cytoplasmic fractions as well as total cell mRNAs showed a rapid decay, with up to 50% concentration reductions at infection times at which influenza virus M1 mRNA was still not detectable. In contrast, these cellular mRNAs were stable in uninfected cells. To ascertain the possible role of mRNA degradation in the cellular protein synthesis shutoff, the kinetics of protein synthesis in infected cells were examined by two-dimensional gel electrophoresis of extracts pulse-labelled at several times after viral infection. The synthesis of the cellular proteins was reduced, showing kinetics paralleling those of mRNA decay. It is proposed that influenza virus infection induces the destabilization of mRNAs and that this mRNA degradation is, at least in part, responsible for cellular protein synthesis shutoff.
Received 11 September 1991;
accepted 18 November 1991.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-73-3-575</identifier><identifier>PMID: 1545220</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Actins - genetics ; Actins - metabolism ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Humans ; Influenza A virus - genetics ; Influenza A virus - metabolism ; influenza virus ; Influenza, Human - genetics ; Influenza, Human - metabolism ; Microbiology ; Protein Biosynthesis ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; RNA, Messenger - metabolism ; RNA, Viral - genetics ; RNA, Viral - metabolism ; Subcellular Fractions - chemistry ; Tubulin - genetics ; Tubulin - metabolism ; Vimentin - genetics ; Vimentin - metabolism ; Virology</subject><ispartof>Journal of general virology, 1992-03, Vol.73 (3), p.575-581</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3495-61b17930bf4305072f9d6b74707c522a578fd2d8b2bb972160b6882e8678efc93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5145988$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1545220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beloso, Ana</creatorcontrib><creatorcontrib>Martinez, Concepcion</creatorcontrib><creatorcontrib>Valcarcel, Juan</creatorcontrib><creatorcontrib>Santaren, Juan Fernandez</creatorcontrib><creatorcontrib>Ortin, Juan</creatorcontrib><title>Degradation of cellular mRNA during influenza virus infection: its possible role in protein synthesis shutoff</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Centro National de Biotecnología (CSIC), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid
and 2 Centro de Biología Molecular (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain
The kinetics of cellular mRNA decay in influenza virus-infected cells have been studied by means of blot hybridization using as probes cloned cDNAs of - and -actin, - and -tubulin and vimentin. Both cellular mRNAs isolated from the cytoplasmic fractions as well as total cell mRNAs showed a rapid decay, with up to 50% concentration reductions at infection times at which influenza virus M1 mRNA was still not detectable. In contrast, these cellular mRNAs were stable in uninfected cells. To ascertain the possible role of mRNA degradation in the cellular protein synthesis shutoff, the kinetics of protein synthesis in infected cells were examined by two-dimensional gel electrophoresis of extracts pulse-labelled at several times after viral infection. The synthesis of the cellular proteins was reduced, showing kinetics paralleling those of mRNA decay. It is proposed that influenza virus infection induces the destabilization of mRNAs and that this mRNA degradation is, at least in part, responsible for cellular protein synthesis shutoff.
Received 11 September 1991;
accepted 18 November 1991.</description><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - metabolism</subject><subject>influenza virus</subject><subject>Influenza, Human - genetics</subject><subject>Influenza, Human - metabolism</subject><subject>Microbiology</subject><subject>Protein Biosynthesis</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - metabolism</subject><subject>Subcellular Fractions - chemistry</subject><subject>Tubulin - genetics</subject><subject>Tubulin - metabolism</subject><subject>Vimentin - genetics</subject><subject>Vimentin - metabolism</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1LHDEUhkNRtuvaP1Ao5ELq1Wg-J4l3orYVxILY65DMJLspM5ltMlOxv94Mu6yXvckhnOc9Xy8AnzG6wEipS4QIqTDFohK0ohUX_ANYYlbzipT0EVgegI_gJOffCGHGuFiABeaME4KWoL9162RaM4YhwsHDxnXd1JkE-6fHa9hOKcQ1DNF3k4v_DPwb0pTnv2tmxRUMY4bbIedgOwfTUJ4Q4TYNoysxv8Zx43LIMG-mcfD-FBx702X3aR9X4Ne3u-ebH9XDz-_3N9cPVUOZ4lWNLRaKIusZRRwJ4lVbW8EEEk0Z23AhfUtaaYm1ShBcI1tLSZyshXS-UXQFvu7qlkn-TC6Pug95Xs1EN0xZCyKpIoz8F8R1zbEgooBkBzapbJuc19sUepNeNUZ6NkPPt9bzrbWgmupiRhF92VefbO_ad8nu-iV_ts-b3JjOJxObkA8Yx4wrKQt2vsM2Yb15CcnptYt9KJPYMOhiyaHhG-8bn4I</recordid><startdate>199203</startdate><enddate>199203</enddate><creator>Beloso, Ana</creator><creator>Martinez, Concepcion</creator><creator>Valcarcel, Juan</creator><creator>Santaren, Juan Fernandez</creator><creator>Ortin, Juan</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199203</creationdate><title>Degradation of cellular mRNA during influenza virus infection: its possible role in protein synthesis shutoff</title><author>Beloso, Ana ; Martinez, Concepcion ; Valcarcel, Juan ; Santaren, Juan Fernandez ; Ortin, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3495-61b17930bf4305072f9d6b74707c522a578fd2d8b2bb972160b6882e8678efc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - metabolism</topic><topic>influenza virus</topic><topic>Influenza, Human - genetics</topic><topic>Influenza, Human - metabolism</topic><topic>Microbiology</topic><topic>Protein Biosynthesis</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - metabolism</topic><topic>Subcellular Fractions - chemistry</topic><topic>Tubulin - genetics</topic><topic>Tubulin - metabolism</topic><topic>Vimentin - genetics</topic><topic>Vimentin - metabolism</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beloso, Ana</creatorcontrib><creatorcontrib>Martinez, Concepcion</creatorcontrib><creatorcontrib>Valcarcel, Juan</creatorcontrib><creatorcontrib>Santaren, Juan Fernandez</creatorcontrib><creatorcontrib>Ortin, Juan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beloso, Ana</au><au>Martinez, Concepcion</au><au>Valcarcel, Juan</au><au>Santaren, Juan Fernandez</au><au>Ortin, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradation of cellular mRNA during influenza virus infection: its possible role in protein synthesis shutoff</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1992-03</date><risdate>1992</risdate><volume>73</volume><issue>3</issue><spage>575</spage><epage>581</epage><pages>575-581</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Centro National de Biotecnología (CSIC), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid
and 2 Centro de Biología Molecular (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain
The kinetics of cellular mRNA decay in influenza virus-infected cells have been studied by means of blot hybridization using as probes cloned cDNAs of - and -actin, - and -tubulin and vimentin. Both cellular mRNAs isolated from the cytoplasmic fractions as well as total cell mRNAs showed a rapid decay, with up to 50% concentration reductions at infection times at which influenza virus M1 mRNA was still not detectable. In contrast, these cellular mRNAs were stable in uninfected cells. To ascertain the possible role of mRNA degradation in the cellular protein synthesis shutoff, the kinetics of protein synthesis in infected cells were examined by two-dimensional gel electrophoresis of extracts pulse-labelled at several times after viral infection. The synthesis of the cellular proteins was reduced, showing kinetics paralleling those of mRNA decay. It is proposed that influenza virus infection induces the destabilization of mRNAs and that this mRNA degradation is, at least in part, responsible for cellular protein synthesis shutoff.
Received 11 September 1991;
accepted 18 November 1991.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>1545220</pmid><doi>10.1099/0022-1317-73-3-575</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Actins - genetics Actins - metabolism Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene Expression Regulation Humans Influenza A virus - genetics Influenza A virus - metabolism influenza virus Influenza, Human - genetics Influenza, Human - metabolism Microbiology Protein Biosynthesis Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA, Messenger - metabolism RNA, Viral - genetics RNA, Viral - metabolism Subcellular Fractions - chemistry Tubulin - genetics Tubulin - metabolism Vimentin - genetics Vimentin - metabolism Virology |
| title | Degradation of cellular mRNA during influenza virus infection: its possible role in protein synthesis shutoff |
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