Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase
A 4.1-kb EcoRI fragment which includes the gene ( gldA) encoding a glycerol dehydrogenase (GIDH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var. non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to Escherichia coli glycerol kinase...
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| Veröffentlicht in: | Gene 1992-01, Vol.110 (1), p.9-16 |
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| creator | Mallinder, Phillip R. Pritchard, Andrew Moir, Anne |
| description | A 4.1-kb
EcoRI fragment which includes the gene (
gldA) encoding a glycerol dehydrogenase (GIDH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from
Bacillus stearothermophilus var.
non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to
Escherichia coli glycerol kinase (
glpK) and glycerol-3-phosphate dehydrogenase (
glpD) mutants. Sequencing suggests that the
gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of G1DH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent G1DH from
B. stearothermophilis RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of
Zymomonas mobilis and the butanol dehydrogenase of
Clostridium acetobutylicum. |
| doi_str_mv | 10.1016/0378-1119(92)90438-U |
| format | Article |
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EcoRI fragment which includes the gene (
gldA) encoding a glycerol dehydrogenase (GIDH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from
Bacillus stearothermophilus var.
non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to
Escherichia coli glycerol kinase (
glpK) and glycerol-3-phosphate dehydrogenase (
glpD) mutants. Sequencing suggests that the
gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of G1DH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent G1DH from
B. stearothermophilis RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of
Zymomonas mobilis and the butanol dehydrogenase of
Clostridium acetobutylicum.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(92)90438-U</identifier><identifier>PMID: 1339360</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Bacillus stearothermophilus ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - metabolism ; Base Sequence ; Cloning, Molecular ; Clostridium ; DNA ; genes ; Genes, Bacterial ; Genetic Complementation Test ; Geobacillus stearothermophilus - enzymology ; Geobacillus stearothermophilus - genetics ; Geobacillus stearothermophilus - metabolism ; gldA gene ; glycerol dehydrogenase ; kinase ; Molecular Sequence Data ; Mutation ; nucleotide sequence ; Open Reading Frames ; predictions ; Sequence Homology, Nucleic Acid ; Sugar Alcohol Dehydrogenases - biosynthesis ; Sugar Alcohol Dehydrogenases - genetics ; Sugar Alcohol Dehydrogenases - metabolism ; thermophile ; Zymomonas</subject><ispartof>Gene, 1992-01, Vol.110 (1), p.9-16</ispartof><rights>1992</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-660f4f661dbe3abb75bb78af4adb78b29dc81d75567fdba8d563cc963674d4bf3</citedby><cites>FETCH-LOGICAL-c388t-660f4f661dbe3abb75bb78af4adb78b29dc81d75567fdba8d563cc963674d4bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(92)90438-U$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1339360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mallinder, Phillip R.</creatorcontrib><creatorcontrib>Pritchard, Andrew</creatorcontrib><creatorcontrib>Moir, Anne</creatorcontrib><title>Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase</title><title>Gene</title><addtitle>Gene</addtitle><description>A 4.1-kb
EcoRI fragment which includes the gene (
gldA) encoding a glycerol dehydrogenase (GIDH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from
Bacillus stearothermophilus var.
non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to
Escherichia coli glycerol kinase (
glpK) and glycerol-3-phosphate dehydrogenase (
glpD) mutants. Sequencing suggests that the
gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of G1DH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent G1DH from
B. stearothermophilis RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of
Zymomonas mobilis and the butanol dehydrogenase of
Clostridium acetobutylicum.</description><subject>Amino Acid Sequence</subject><subject>Bacillus stearothermophilus</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Clostridium</subject><subject>DNA</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>Geobacillus stearothermophilus - enzymology</subject><subject>Geobacillus stearothermophilus - genetics</subject><subject>Geobacillus stearothermophilus - metabolism</subject><subject>gldA gene</subject><subject>glycerol dehydrogenase</subject><subject>kinase</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>nucleotide sequence</subject><subject>Open Reading Frames</subject><subject>predictions</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Sugar Alcohol Dehydrogenases - biosynthesis</subject><subject>Sugar Alcohol Dehydrogenases - genetics</subject><subject>Sugar Alcohol Dehydrogenases - metabolism</subject><subject>thermophile</subject><subject>Zymomonas</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU2LFDEQDaKs4-o_UMhJ3EOvSac7nVwEHdYPWPDinEN1UpmJdCdj0rMwHvztZnYWvWlBUVD13it4j5CXnF1zxuVbJgbVcM71G91eadYJ1WwekRVXg24YE-oxWf2BPCXPSvnOavV9e0EuuBBaSLYiv9ZTiiFuKURH7Q4y2AVz-AlLSJEmT4FuMSL1Oc30A9gwTYdCy4KQ07LDPKf9LpxWd5CvaUyxcQHKUum2LjHa5O7V6XY6Wsxpog53R5dTVYWCz8kTD1PBFw_zkmw-3nxbf25uv376sn5_21ih1NJIyXznpeRuRAHjOPS1FfgOXJ1jq51V3A19LwfvRlCul8JaLYUcOteNXlyS12fdfU4_DlgWM4dicZogYjoUM7RKDLxt_wvksmW656wCuzPQ5lRKRm_2OcyQj4Yzc8rHnMw3J_ONbs19PmZTaa8e9A_jjO4v6RxIvb8737G6cRcwm2JDtRFdyGgX41L494PfcDKiwQ</recordid><startdate>19920102</startdate><enddate>19920102</enddate><creator>Mallinder, Phillip R.</creator><creator>Pritchard, Andrew</creator><creator>Moir, Anne</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19920102</creationdate><title>Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase</title><author>Mallinder, Phillip R. ; Pritchard, Andrew ; Moir, Anne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-660f4f661dbe3abb75bb78af4adb78b29dc81d75567fdba8d563cc963674d4bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Bacillus stearothermophilus</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Clostridium</topic><topic>DNA</topic><topic>genes</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>Geobacillus stearothermophilus - enzymology</topic><topic>Geobacillus stearothermophilus - genetics</topic><topic>Geobacillus stearothermophilus - metabolism</topic><topic>gldA gene</topic><topic>glycerol dehydrogenase</topic><topic>kinase</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>nucleotide sequence</topic><topic>Open Reading Frames</topic><topic>predictions</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Sugar Alcohol Dehydrogenases - biosynthesis</topic><topic>Sugar Alcohol Dehydrogenases - genetics</topic><topic>Sugar Alcohol Dehydrogenases - metabolism</topic><topic>thermophile</topic><topic>Zymomonas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mallinder, Phillip R.</creatorcontrib><creatorcontrib>Pritchard, Andrew</creatorcontrib><creatorcontrib>Moir, Anne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mallinder, Phillip R.</au><au>Pritchard, Andrew</au><au>Moir, Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1992-01-02</date><risdate>1992</risdate><volume>110</volume><issue>1</issue><spage>9</spage><epage>16</epage><pages>9-16</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A 4.1-kb
EcoRI fragment which includes the gene (
gldA) encoding a glycerol dehydrogenase (GIDH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from
Bacillus stearothermophilus var.
non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to
Escherichia coli glycerol kinase (
glpK) and glycerol-3-phosphate dehydrogenase (
glpD) mutants. Sequencing suggests that the
gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of G1DH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent G1DH from
B. stearothermophilis RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of
Zymomonas mobilis and the butanol dehydrogenase of
Clostridium acetobutylicum.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>1339360</pmid><doi>10.1016/0378-1119(92)90438-U</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1992-01, Vol.110 (1), p.9-16 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72837122 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Bacillus stearothermophilus Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Base Sequence Cloning, Molecular Clostridium DNA genes Genes, Bacterial Genetic Complementation Test Geobacillus stearothermophilus - enzymology Geobacillus stearothermophilus - genetics Geobacillus stearothermophilus - metabolism gldA gene glycerol dehydrogenase kinase Molecular Sequence Data Mutation nucleotide sequence Open Reading Frames predictions Sequence Homology, Nucleic Acid Sugar Alcohol Dehydrogenases - biosynthesis Sugar Alcohol Dehydrogenases - genetics Sugar Alcohol Dehydrogenases - metabolism thermophile Zymomonas |
| title | Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase |
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