Molecular cloning and characterization of a constitutively expressed heat‐shock‐cognate hsc71 gene from rainbow trout
A rainbow trout major heat‐shock‐protein‐like gene (hsp 70) and corresponding cDNA clones were isolated by hybridization to heterologous hsp70 probes. DNA sequencing revealed that this gene is structurally similar to a mammalian heat‐shock‐cognate hsc70 gene and consists of eight introns. Northern b...
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| Veröffentlicht in: | European journal of biochemistry 1992-03, Vol.204 (2), p.893-900 |
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| container_title | European journal of biochemistry |
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| creator | ZAFARULLAH, Muhammad WISNIEWSKI, Jan SHWORAK, Nicholas W. SCHIEMAN, Steven MISRA, Santosh GEDAMU, Lashitew |
| description | A rainbow trout major heat‐shock‐protein‐like gene (hsp 70) and corresponding cDNA clones were isolated by hybridization to heterologous hsp70 probes. DNA sequencing revealed that this gene is structurally similar to a mammalian heat‐shock‐cognate hsc70 gene and consists of eight introns. Northern blot and primer extension analyses showed that the corresponding mRNA is constitutively abundant in different trout tissues and salmonid cell lines. Fragments of the isolated gene containing the −900 –+30 and −217 –+58 sequence were linked to a bacterial chloramphenicol acetyltransferase reporter gene and transiently transfected into salmonid cells. The expression pattern of these constructs supports our conclusion that the isolated genomic and cDNA clones correspond to a trout heat‐shock‐cognate hsc70 gene. |
| doi_str_mv | 10.1111/j.1432-1033.1992.tb16709.x |
| format | Article |
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DNA sequencing revealed that this gene is structurally similar to a mammalian heat‐shock‐cognate hsc70 gene and consists of eight introns. Northern blot and primer extension analyses showed that the corresponding mRNA is constitutively abundant in different trout tissues and salmonid cell lines. Fragments of the isolated gene containing the −900 –+30 and −217 –+58 sequence were linked to a bacterial chloramphenicol acetyltransferase reporter gene and transiently transfected into salmonid cells. The expression pattern of these constructs supports our conclusion that the isolated genomic and cDNA clones correspond to a trout heat‐shock‐cognate hsc70 gene.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1992.tb16709.x</identifier><identifier>PMID: 1371753</identifier><identifier>CODEN: EJBCAI</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; Chloramphenicol O-Acetyltransferase - genetics ; Cloning, Molecular ; DNA - genetics ; DNA - isolation & purification ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Heat-Shock Proteins - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Oncorhynchus mykiss ; Promoter Regions, Genetic ; Restriction Mapping ; RNA - genetics ; Sequence Homology, Nucleic Acid ; Transcription, Genetic ; Transfection ; Trout - genetics</subject><ispartof>European journal of biochemistry, 1992-03, Vol.204 (2), p.893-900</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5483-d79dedcefa5edea49b2ab2cb5f21f54d90cda132224f216e49728d3b7f659cc33</citedby><cites>FETCH-LOGICAL-c5483-d79dedcefa5edea49b2ab2cb5f21f54d90cda132224f216e49728d3b7f659cc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5284371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1371753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZAFARULLAH, Muhammad</creatorcontrib><creatorcontrib>WISNIEWSKI, Jan</creatorcontrib><creatorcontrib>SHWORAK, Nicholas W.</creatorcontrib><creatorcontrib>SCHIEMAN, Steven</creatorcontrib><creatorcontrib>MISRA, Santosh</creatorcontrib><creatorcontrib>GEDAMU, Lashitew</creatorcontrib><title>Molecular cloning and characterization of a constitutively expressed heat‐shock‐cognate hsc71 gene from rainbow trout</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>A rainbow trout major heat‐shock‐protein‐like gene (hsp 70) and corresponding cDNA clones were isolated by hybridization to heterologous hsp70 probes. DNA sequencing revealed that this gene is structurally similar to a mammalian heat‐shock‐cognate hsc70 gene and consists of eight introns. Northern blot and primer extension analyses showed that the corresponding mRNA is constitutively abundant in different trout tissues and salmonid cell lines. Fragments of the isolated gene containing the −900 –+30 and −217 –+58 sequence were linked to a bacterial chloramphenicol acetyltransferase reporter gene and transiently transfected into salmonid cells. The expression pattern of these constructs supports our conclusion that the isolated genomic and cDNA clones correspond to a trout heat‐shock‐cognate hsc70 gene.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Oncorhynchus mykiss</subject><subject>Promoter Regions, Genetic</subject><subject>Restriction Mapping</subject><subject>RNA - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Trout - genetics</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkcuKFDEUhoMoYzv6CEIQcVdlLnWLGxmHGRVGXKjrcCo51Z22OmmTlNPtykfwGX0Sq-lmXIrZHMj_nQt8hDzjrOTze7kueSVFwZmUJVdKlLnnTctUubtHFnfRfbJgjFeFUHXzkDxKac0Ya1TTnpEzLlve1nJB9h_CiGYaIVIzBu_8koK31KwggskY3Q_ILngaBgrUBJ-yy1N233HcU9xtI6aElq4Q8u-fv9IqmK9zNWHpISNdJdNyukSPdIhhQyM434dbmmOY8mPyYIAx4ZNTPSdfrq8-X74rbj6-fX95cVOYuupkYVtl0RocoEaLUKleQC9MXw-CD3VlFTMWuBRCVPNPg5VqRWdl3w5NrYyR8py8OM7dxvBtwpT1xiWD4wgew5T0jEspOv5PkDe8qzqlZvDVETQxpBRx0NvoNhD3mjN9EKTX-mBBHyzogyB9EqR3c_PT05ap36D923o0MufPTzkkA-MQwRuX7rBadNVMztjrI3brRtz_xwH6-urNp05J-Qfa2LJs</recordid><startdate>199203</startdate><enddate>199203</enddate><creator>ZAFARULLAH, Muhammad</creator><creator>WISNIEWSKI, Jan</creator><creator>SHWORAK, Nicholas W.</creator><creator>SCHIEMAN, Steven</creator><creator>MISRA, Santosh</creator><creator>GEDAMU, Lashitew</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199203</creationdate><title>Molecular cloning and characterization of a constitutively expressed heat‐shock‐cognate hsc71 gene from rainbow trout</title><author>ZAFARULLAH, Muhammad ; WISNIEWSKI, Jan ; SHWORAK, Nicholas W. ; SCHIEMAN, Steven ; MISRA, Santosh ; GEDAMU, Lashitew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5483-d79dedcefa5edea49b2ab2cb5f21f54d90cda132224f216e49728d3b7f659cc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Oncorhynchus mykiss</topic><topic>Promoter Regions, Genetic</topic><topic>Restriction Mapping</topic><topic>RNA - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Trout - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZAFARULLAH, Muhammad</creatorcontrib><creatorcontrib>WISNIEWSKI, Jan</creatorcontrib><creatorcontrib>SHWORAK, Nicholas W.</creatorcontrib><creatorcontrib>SCHIEMAN, Steven</creatorcontrib><creatorcontrib>MISRA, Santosh</creatorcontrib><creatorcontrib>GEDAMU, Lashitew</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZAFARULLAH, Muhammad</au><au>WISNIEWSKI, Jan</au><au>SHWORAK, Nicholas W.</au><au>SCHIEMAN, Steven</au><au>MISRA, Santosh</au><au>GEDAMU, Lashitew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and characterization of a constitutively expressed heat‐shock‐cognate hsc71 gene from rainbow trout</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1992-03</date><risdate>1992</risdate><volume>204</volume><issue>2</issue><spage>893</spage><epage>900</epage><pages>893-900</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><coden>EJBCAI</coden><abstract>A rainbow trout major heat‐shock‐protein‐like gene (hsp 70) and corresponding cDNA clones were isolated by hybridization to heterologous hsp70 probes. DNA sequencing revealed that this gene is structurally similar to a mammalian heat‐shock‐cognate hsc70 gene and consists of eight introns. Northern blot and primer extension analyses showed that the corresponding mRNA is constitutively abundant in different trout tissues and salmonid cell lines. Fragments of the isolated gene containing the −900 –+30 and −217 –+58 sequence were linked to a bacterial chloramphenicol acetyltransferase reporter gene and transiently transfected into salmonid cells. The expression pattern of these constructs supports our conclusion that the isolated genomic and cDNA clones correspond to a trout heat‐shock‐cognate hsc70 gene.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1371753</pmid><doi>10.1111/j.1432-1033.1992.tb16709.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of biochemistry, 1992-03, Vol.204 (2), p.893-900 |
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| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Southern Chloramphenicol O-Acetyltransferase - genetics Cloning, Molecular DNA - genetics DNA - isolation & purification Freshwater Fundamental and applied biological sciences. Psychology Genes. Genome Heat-Shock Proteins - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization Oncorhynchus mykiss Promoter Regions, Genetic Restriction Mapping RNA - genetics Sequence Homology, Nucleic Acid Transcription, Genetic Transfection Trout - genetics |
| title | Molecular cloning and characterization of a constitutively expressed heat‐shock‐cognate hsc71 gene from rainbow trout |
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