Trans-synaptic increase of hypoxic tolerance in hippocampus upon physical challenge with two-photon microscopy
Neuronal hypoxic tolerance is modulated by preceding challenges. We investigated hypoxic tolerance in CA1 pyramidal cells of murine hippocampal slices upon preceding physical challenge with two‐photon illumination in close spatial proximity to the recorded area, at distant presynaptic neurons, or pr...
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| Veröffentlicht in: | Hippocampus 2002, Vol.12 (6), p.765-773 |
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| creator | Büchner, Maren Huber, Roman Riepe, Matthias W. |
| description | Neuronal hypoxic tolerance is modulated by preceding challenges. We investigated hypoxic tolerance in CA1 pyramidal cells of murine hippocampal slices upon preceding physical challenge with two‐photon illumination in close spatial proximity to the recorded area, at distant presynaptic neurons, or preceding chemical treatment with acetylsalicylic acid while zinc fluorescence was assessed with fluorescence measurement upon staining with N‐(6‐methoxy‐8‐quinolyl)‐para‐toluenesulfonamide (TSQ). Posthypoxic recovery (15 min hypoxia, 45 min recovery) of CA1 population spike amplitude (PSAP) upon stimulation of Schaffer collaterals in hippocampal region CA3 was 20 ± 38% (mean ±SD; n = 15) in control slices. At the end of hypoxia, zinc fluorescence increased to 120 ± 16% (P < 0.05 to control) in slices that later recovered and 141 ± 20% in slices that did not recover (P < 0.01 to control; P < 0.05 compared with returns). Multi‐photon illumination alone was an appropriate physical challenge to improve hypoxic tolerance, even trans‐synaptically. Depending on the number of illuminations posthypoxic PSAP increased up to 84 ± 25% (P < 0.01 to control) upon illumination of hippocampal region CA1 and 85 ± 28% (P < 0.01 to control) upon illumination of CA3. With the latter treatment, zinc fluorescence in CA1 increased to 126 ± 20% before hypoxia (P < 0.05 to control), and no further zinc increase was observed upon subsequent hypoxia. Similar results were obtained upon chemical preconditioning with acetylsalicylate. We conclude that observation of live specimen with multi‐photon imaging alters the physiology of neuronal cell ensembles, including hypoxic tolerance, even trans‐synaptically at long distances from the imaged area. This is mediated in part through endogenous modulation by zinc. Mild zinc increase improves hypoxic tolerance while pronounced increase predicts neuronal cell death. Hippocampus 2002;12:765–773. © 2002 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/hipo.10028 |
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We investigated hypoxic tolerance in CA1 pyramidal cells of murine hippocampal slices upon preceding physical challenge with two‐photon illumination in close spatial proximity to the recorded area, at distant presynaptic neurons, or preceding chemical treatment with acetylsalicylic acid while zinc fluorescence was assessed with fluorescence measurement upon staining with N‐(6‐methoxy‐8‐quinolyl)‐para‐toluenesulfonamide (TSQ). Posthypoxic recovery (15 min hypoxia, 45 min recovery) of CA1 population spike amplitude (PSAP) upon stimulation of Schaffer collaterals in hippocampal region CA3 was 20 ± 38% (mean ±SD; n = 15) in control slices. At the end of hypoxia, zinc fluorescence increased to 120 ± 16% (P < 0.05 to control) in slices that later recovered and 141 ± 20% in slices that did not recover (P < 0.01 to control; P < 0.05 compared with returns). Multi‐photon illumination alone was an appropriate physical challenge to improve hypoxic tolerance, even trans‐synaptically. Depending on the number of illuminations posthypoxic PSAP increased up to 84 ± 25% (P < 0.01 to control) upon illumination of hippocampal region CA1 and 85 ± 28% (P < 0.01 to control) upon illumination of CA3. With the latter treatment, zinc fluorescence in CA1 increased to 126 ± 20% before hypoxia (P < 0.05 to control), and no further zinc increase was observed upon subsequent hypoxia. Similar results were obtained upon chemical preconditioning with acetylsalicylate. We conclude that observation of live specimen with multi‐photon imaging alters the physiology of neuronal cell ensembles, including hypoxic tolerance, even trans‐synaptically at long distances from the imaged area. This is mediated in part through endogenous modulation by zinc. Mild zinc increase improves hypoxic tolerance while pronounced increase predicts neuronal cell death. Hippocampus 2002;12:765–773. © 2002 Wiley‐Liss, Inc.]]></description><identifier>ISSN: 1050-9631</identifier><identifier>EISSN: 1098-1063</identifier><identifier>DOI: 10.1002/hipo.10028</identifier><identifier>PMID: 12542228</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Action Potentials - drug effects ; Action Potentials - physiology ; Aminoquinolines ; Animals ; Aspirin - pharmacology ; cell death ; Chelating Agents - pharmacology ; Fluorescent Dyes ; Hippocampus - drug effects ; Hippocampus - metabolism ; Hippocampus - physiopathology ; Hypoxia-Ischemia, Brain - metabolism ; Hypoxia-Ischemia, Brain - physiopathology ; Ischemic Preconditioning - methods ; Lasers ; Light ; Male ; Mice ; mouse ; Neurons - drug effects ; Neurons - metabolism ; neuroprotection ; Photic Stimulation ; preconditioning ; slice ; Synapses - drug effects ; Synapses - metabolism ; Synaptic Transmission - drug effects ; Synaptic Transmission - physiology ; Tosyl Compounds ; Zinc - metabolism</subject><ispartof>Hippocampus, 2002, Vol.12 (6), p.765-773</ispartof><rights>Copyright © 2002 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3638-d40851f05eecbc46e9292bf32b9a41af691a9fbb20d1c6e312882b4564c0e2143</citedby><cites>FETCH-LOGICAL-c3638-d40851f05eecbc46e9292bf32b9a41af691a9fbb20d1c6e312882b4564c0e2143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fhipo.10028$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fhipo.10028$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4023,27922,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12542228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Büchner, Maren</creatorcontrib><creatorcontrib>Huber, Roman</creatorcontrib><creatorcontrib>Riepe, Matthias W.</creatorcontrib><title>Trans-synaptic increase of hypoxic tolerance in hippocampus upon physical challenge with two-photon microscopy</title><title>Hippocampus</title><addtitle>Hippocampus</addtitle><description><![CDATA[Neuronal hypoxic tolerance is modulated by preceding challenges. We investigated hypoxic tolerance in CA1 pyramidal cells of murine hippocampal slices upon preceding physical challenge with two‐photon illumination in close spatial proximity to the recorded area, at distant presynaptic neurons, or preceding chemical treatment with acetylsalicylic acid while zinc fluorescence was assessed with fluorescence measurement upon staining with N‐(6‐methoxy‐8‐quinolyl)‐para‐toluenesulfonamide (TSQ). Posthypoxic recovery (15 min hypoxia, 45 min recovery) of CA1 population spike amplitude (PSAP) upon stimulation of Schaffer collaterals in hippocampal region CA3 was 20 ± 38% (mean ±SD; n = 15) in control slices. At the end of hypoxia, zinc fluorescence increased to 120 ± 16% (P < 0.05 to control) in slices that later recovered and 141 ± 20% in slices that did not recover (P < 0.01 to control; P < 0.05 compared with returns). Multi‐photon illumination alone was an appropriate physical challenge to improve hypoxic tolerance, even trans‐synaptically. Depending on the number of illuminations posthypoxic PSAP increased up to 84 ± 25% (P < 0.01 to control) upon illumination of hippocampal region CA1 and 85 ± 28% (P < 0.01 to control) upon illumination of CA3. With the latter treatment, zinc fluorescence in CA1 increased to 126 ± 20% before hypoxia (P < 0.05 to control), and no further zinc increase was observed upon subsequent hypoxia. Similar results were obtained upon chemical preconditioning with acetylsalicylate. We conclude that observation of live specimen with multi‐photon imaging alters the physiology of neuronal cell ensembles, including hypoxic tolerance, even trans‐synaptically at long distances from the imaged area. This is mediated in part through endogenous modulation by zinc. Mild zinc increase improves hypoxic tolerance while pronounced increase predicts neuronal cell death. Hippocampus 2002;12:765–773. © 2002 Wiley‐Liss, Inc.]]></description><subject>Action Potentials - drug effects</subject><subject>Action Potentials - physiology</subject><subject>Aminoquinolines</subject><subject>Animals</subject><subject>Aspirin - pharmacology</subject><subject>cell death</subject><subject>Chelating Agents - pharmacology</subject><subject>Fluorescent Dyes</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - physiopathology</subject><subject>Hypoxia-Ischemia, Brain - metabolism</subject><subject>Hypoxia-Ischemia, Brain - physiopathology</subject><subject>Ischemic Preconditioning - methods</subject><subject>Lasers</subject><subject>Light</subject><subject>Male</subject><subject>Mice</subject><subject>mouse</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>neuroprotection</subject><subject>Photic Stimulation</subject><subject>preconditioning</subject><subject>slice</subject><subject>Synapses - drug effects</subject><subject>Synapses - metabolism</subject><subject>Synaptic Transmission - drug effects</subject><subject>Synaptic Transmission - physiology</subject><subject>Tosyl Compounds</subject><subject>Zinc - metabolism</subject><issn>1050-9631</issn><issn>1098-1063</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFv1DAQhS0EoqVw4QcgnzhUCszYSdY-ogq6rUrbQxFHy_FOiCGJTZxom3_fbHdLb5zmaeabN6PH2HuETwggPjc-hkelXrBjBK0yhFK-3OkCMl1KPGJvUvoNgFgAvGZHKIpcCKGOWX832D5lae5tHL3jvncD2UQ81LyZY7hfemNoaaEcLVO-HIvB2S5OiU8x9Dw2c_LOttw1tm2p_0V868eGj9uQxSaMC9J5N4TkQpzfsle1bRO9O9QT9uPb17uzdXZ1c35x9uUqc7KUKtvkoAqsoSBylctL0kKLqpai0jZHW5cara6rSsAGXUkShVKiyosyd0ACc3nCPu594xD-TpRG0_nkqG1tT2FKZiWU0BpgAU_34O7DNFBt4uA7O8wGwewyNbt0H5Va4A8H16nqaPOMHuJcANwDW9_S_B8rs764vXkyzfY7Po10_2_HDn9MuZKrwvy8PjfFCi7X6vulQfkAvYOWIQ</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Büchner, Maren</creator><creator>Huber, Roman</creator><creator>Riepe, Matthias W.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Trans-synaptic increase of hypoxic tolerance in hippocampus upon physical challenge with two-photon microscopy</title><author>Büchner, Maren ; Huber, Roman ; Riepe, Matthias W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3638-d40851f05eecbc46e9292bf32b9a41af691a9fbb20d1c6e312882b4564c0e2143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Action Potentials - drug effects</topic><topic>Action Potentials - physiology</topic><topic>Aminoquinolines</topic><topic>Animals</topic><topic>Aspirin - pharmacology</topic><topic>cell death</topic><topic>Chelating Agents - pharmacology</topic><topic>Fluorescent Dyes</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - physiopathology</topic><topic>Hypoxia-Ischemia, Brain - metabolism</topic><topic>Hypoxia-Ischemia, Brain - physiopathology</topic><topic>Ischemic Preconditioning - methods</topic><topic>Lasers</topic><topic>Light</topic><topic>Male</topic><topic>Mice</topic><topic>mouse</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>neuroprotection</topic><topic>Photic Stimulation</topic><topic>preconditioning</topic><topic>slice</topic><topic>Synapses - drug effects</topic><topic>Synapses - metabolism</topic><topic>Synaptic Transmission - drug effects</topic><topic>Synaptic Transmission - physiology</topic><topic>Tosyl Compounds</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Büchner, Maren</creatorcontrib><creatorcontrib>Huber, Roman</creatorcontrib><creatorcontrib>Riepe, Matthias W.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hippocampus</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Büchner, Maren</au><au>Huber, Roman</au><au>Riepe, Matthias W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-synaptic increase of hypoxic tolerance in hippocampus upon physical challenge with two-photon microscopy</atitle><jtitle>Hippocampus</jtitle><addtitle>Hippocampus</addtitle><date>2002</date><risdate>2002</risdate><volume>12</volume><issue>6</issue><spage>765</spage><epage>773</epage><pages>765-773</pages><issn>1050-9631</issn><eissn>1098-1063</eissn><abstract><![CDATA[Neuronal hypoxic tolerance is modulated by preceding challenges. We investigated hypoxic tolerance in CA1 pyramidal cells of murine hippocampal slices upon preceding physical challenge with two‐photon illumination in close spatial proximity to the recorded area, at distant presynaptic neurons, or preceding chemical treatment with acetylsalicylic acid while zinc fluorescence was assessed with fluorescence measurement upon staining with N‐(6‐methoxy‐8‐quinolyl)‐para‐toluenesulfonamide (TSQ). Posthypoxic recovery (15 min hypoxia, 45 min recovery) of CA1 population spike amplitude (PSAP) upon stimulation of Schaffer collaterals in hippocampal region CA3 was 20 ± 38% (mean ±SD; n = 15) in control slices. At the end of hypoxia, zinc fluorescence increased to 120 ± 16% (P < 0.05 to control) in slices that later recovered and 141 ± 20% in slices that did not recover (P < 0.01 to control; P < 0.05 compared with returns). Multi‐photon illumination alone was an appropriate physical challenge to improve hypoxic tolerance, even trans‐synaptically. Depending on the number of illuminations posthypoxic PSAP increased up to 84 ± 25% (P < 0.01 to control) upon illumination of hippocampal region CA1 and 85 ± 28% (P < 0.01 to control) upon illumination of CA3. With the latter treatment, zinc fluorescence in CA1 increased to 126 ± 20% before hypoxia (P < 0.05 to control), and no further zinc increase was observed upon subsequent hypoxia. Similar results were obtained upon chemical preconditioning with acetylsalicylate. We conclude that observation of live specimen with multi‐photon imaging alters the physiology of neuronal cell ensembles, including hypoxic tolerance, even trans‐synaptically at long distances from the imaged area. This is mediated in part through endogenous modulation by zinc. Mild zinc increase improves hypoxic tolerance while pronounced increase predicts neuronal cell death. Hippocampus 2002;12:765–773. © 2002 Wiley‐Liss, Inc.]]></abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>12542228</pmid><doi>10.1002/hipo.10028</doi><tpages>9</tpages></addata></record> |
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| subjects | Action Potentials - drug effects Action Potentials - physiology Aminoquinolines Animals Aspirin - pharmacology cell death Chelating Agents - pharmacology Fluorescent Dyes Hippocampus - drug effects Hippocampus - metabolism Hippocampus - physiopathology Hypoxia-Ischemia, Brain - metabolism Hypoxia-Ischemia, Brain - physiopathology Ischemic Preconditioning - methods Lasers Light Male Mice mouse Neurons - drug effects Neurons - metabolism neuroprotection Photic Stimulation preconditioning slice Synapses - drug effects Synapses - metabolism Synaptic Transmission - drug effects Synaptic Transmission - physiology Tosyl Compounds Zinc - metabolism |
| title | Trans-synaptic increase of hypoxic tolerance in hippocampus upon physical challenge with two-photon microscopy |
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