Expression of Receptors for Granulocyte Colony-Stimulating Factor on Neutrophils From Patients With Severe Congenital Neutropenia and Cyclic Neutropenia
We studied granulocyte colony-stimulating factor (G-CSF) binding sites on neutrophils from patients with severe congenital neutropenia (SCN; Kostmann-syndrome) and cyclic neutropenia (CN) during treatment with recombinant human (rh) G-CSF. G-CSF receptor expression was measured by scatchard analysis...
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| Veröffentlicht in: | Blood 1992-03, Vol.79 (5), p.1144-1147 |
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| creator | Kyas, Urte Pietsch, Torsten Welte, Karl |
| description | We studied granulocyte colony-stimulating factor (G-CSF) binding sites on neutrophils from patients with severe congenital neutropenia (SCN; Kostmann-syndrome) and cyclic neutropenia (CN) during treatment with recombinant human (rh) G-CSF. G-CSF receptor expression was measured by scatchard analysis. Neutrophils from six healthy controls expressed between 480 and 1,210 binding sites per cell, whereas neutrophils from five SCN patients expressed increased numbers of G-CSF binding sites ranging between 2,100 and 3,900 per cell. Neutrophils from four patients with
CN expressed 350 to 1,600 binding sites per cell. The affinity of rhG-CSF to its receptor was similar in patients and controls. These data suggest that SCN patients and CN patients are not defective in G-CSF receptor expression as judged by the numbers of G-CSF binding sites and binding affinity; however, we cannot exclude defects in parts of the G-CSF receptor that may be involved in the signal transduction pathway. |
| doi_str_mv | 10.1182/blood.V79.5.1144.1144 |
| format | Article |
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CN expressed 350 to 1,600 binding sites per cell. The affinity of rhG-CSF to its receptor was similar in patients and controls. These data suggest that SCN patients and CN patients are not defective in G-CSF receptor expression as judged by the numbers of G-CSF binding sites and binding affinity; however, we cannot exclude defects in parts of the G-CSF receptor that may be involved in the signal transduction pathway.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V79.5.1144.1144</identifier><identifier>PMID: 1371412</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Child ; Female ; Granulocyte Colony-Stimulating Factor - therapeutic use ; Humans ; Male ; Malformations of the nervous system ; Medical sciences ; Neurology ; Neutropenia - blood ; Neutropenia - congenital ; Neutropenia - drug therapy ; Neutrophils - metabolism ; Receptors, Granulocyte Colony-Stimulating Factor - metabolism ; Recombinant Proteins - therapeutic use</subject><ispartof>Blood, 1992-03, Vol.79 (5), p.1144-1147</ispartof><rights>1992 American Society of Hematology</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-5712a08806b102da40461c9232ebd89eb4edf0eadface3adb091b5b2dcbceeb73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5576548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1371412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kyas, Urte</creatorcontrib><creatorcontrib>Pietsch, Torsten</creatorcontrib><creatorcontrib>Welte, Karl</creatorcontrib><title>Expression of Receptors for Granulocyte Colony-Stimulating Factor on Neutrophils From Patients With Severe Congenital Neutropenia and Cyclic Neutropenia</title><title>Blood</title><addtitle>Blood</addtitle><description>We studied granulocyte colony-stimulating factor (G-CSF) binding sites on neutrophils from patients with severe congenital neutropenia (SCN; Kostmann-syndrome) and cyclic neutropenia (CN) during treatment with recombinant human (rh) G-CSF. G-CSF receptor expression was measured by scatchard analysis. Neutrophils from six healthy controls expressed between 480 and 1,210 binding sites per cell, whereas neutrophils from five SCN patients expressed increased numbers of G-CSF binding sites ranging between 2,100 and 3,900 per cell. Neutrophils from four patients with
CN expressed 350 to 1,600 binding sites per cell. The affinity of rhG-CSF to its receptor was similar in patients and controls. 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G-CSF receptor expression was measured by scatchard analysis. Neutrophils from six healthy controls expressed between 480 and 1,210 binding sites per cell, whereas neutrophils from five SCN patients expressed increased numbers of G-CSF binding sites ranging between 2,100 and 3,900 per cell. Neutrophils from four patients with
CN expressed 350 to 1,600 binding sites per cell. The affinity of rhG-CSF to its receptor was similar in patients and controls. These data suggest that SCN patients and CN patients are not defective in G-CSF receptor expression as judged by the numbers of G-CSF binding sites and binding affinity; however, we cannot exclude defects in parts of the G-CSF receptor that may be involved in the signal transduction pathway.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>1371412</pmid><doi>10.1182/blood.V79.5.1144.1144</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-4971 |
| ispartof | Blood, 1992-03, Vol.79 (5), p.1144-1147 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adolescent Adult Biological and medical sciences Child Female Granulocyte Colony-Stimulating Factor - therapeutic use Humans Male Malformations of the nervous system Medical sciences Neurology Neutropenia - blood Neutropenia - congenital Neutropenia - drug therapy Neutrophils - metabolism Receptors, Granulocyte Colony-Stimulating Factor - metabolism Recombinant Proteins - therapeutic use |
| title | Expression of Receptors for Granulocyte Colony-Stimulating Factor on Neutrophils From Patients With Severe Congenital Neutropenia and Cyclic Neutropenia |
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