Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes
Objective. To investigate BCP (basic calcium phosphate) crystal—stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes. Methods. The role of protein kinases in BCP crystal—stimulated DNA synthesis was investigated using thymidine incorporation, kinase i...
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| Veröffentlicht in: | Arthritis and rheumatism 1992-03, Vol.35 (3), p.343-350 |
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| container_title | Arthritis and rheumatism |
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| creator | Mitchell, Peter G. Struve, Janine A. Mccarthy, Geraldine M. Cheung, Herman S. |
| description | Objective. To investigate BCP (basic calcium phosphate) crystal—stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes.
Methods. The role of protein kinases in BCP crystal—stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c‐fos and c‐jun message.
Results. BCP crystals stimulated chondrocyte proliferation in a PKC‐dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c‐fos, but not c‐jun.
Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases. |
| doi_str_mv | 10.1002/art.1780350314 |
| format | Article |
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Methods. The role of protein kinases in BCP crystal—stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c‐fos and c‐jun message.
Results. BCP crystals stimulated chondrocyte proliferation in a PKC‐dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c‐fos, but not c‐jun.
Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.</description><identifier>ISSN: 0004-3591</identifier><identifier>EISSN: 1529-0131</identifier><identifier>DOI: 10.1002/art.1780350314</identifier><identifier>PMID: 1311178</identifier><identifier>CODEN: ARHEAW</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Biological and medical sciences ; Calcium Phosphates - pharmacology ; Cartilage, Articular - cytology ; Cell cycle, cell proliferation ; Cell Division - drug effects ; Cell physiology ; Crystallization ; DNA - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Humans ; Membrane Proteins - metabolism ; Microbial Collagenase - genetics ; Molecular and cellular biology ; Protein Kinase C - pharmacology ; Proto-Oncogene Proteins c-fos - genetics ; Proto-Oncogene Proteins c-jun - genetics ; RNA, Messenger ; Transcription, Genetic</subject><ispartof>Arthritis and rheumatism, 1992-03, Vol.35 (3), p.343-350</ispartof><rights>Copyright © 1992 American College of Rheumatology</rights><rights>1992 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3694-cd30ed115611aa4d8afe0326f2616c4f39b43632bb1fb5c49f2836596f407d023</citedby><cites>FETCH-LOGICAL-c3694-cd30ed115611aa4d8afe0326f2616c4f39b43632bb1fb5c49f2836596f407d023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fart.1780350314$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fart.1780350314$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5169055$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1311178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitchell, Peter G.</creatorcontrib><creatorcontrib>Struve, Janine A.</creatorcontrib><creatorcontrib>Mccarthy, Geraldine M.</creatorcontrib><creatorcontrib>Cheung, Herman S.</creatorcontrib><title>Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes</title><title>Arthritis and rheumatism</title><addtitle>Arthritis Rheum</addtitle><description>Objective. To investigate BCP (basic calcium phosphate) crystal—stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes.
Methods. The role of protein kinases in BCP crystal—stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c‐fos and c‐jun message.
Results. BCP crystals stimulated chondrocyte proliferation in a PKC‐dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c‐fos, but not c‐jun.
Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.</description><subject>Biological and medical sciences</subject><subject>Calcium Phosphates - pharmacology</subject><subject>Cartilage, Articular - cytology</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Division - drug effects</subject><subject>Cell physiology</subject><subject>Crystallization</subject><subject>DNA - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Membrane Proteins - metabolism</subject><subject>Microbial Collagenase - genetics</subject><subject>Molecular and cellular biology</subject><subject>Protein Kinase C - pharmacology</subject><subject>Proto-Oncogene Proteins c-fos - genetics</subject><subject>Proto-Oncogene Proteins c-jun - genetics</subject><subject>RNA, Messenger</subject><subject>Transcription, Genetic</subject><issn>0004-3591</issn><issn>1529-0131</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhi0EKmnhyg3JB8Rtg8dfyR5LBQWpEhIq59WsP4iRdzd4doXyG_jTOE1EuXHyeN5nvl7GXoFYgxDyHZZ5DZutUEYo0E_YCoxsGwEKnrKVEEI3yrTwnF0S_ahfqYy6YBdVhlq1Yr_fIyXHHWaXloHvdxPtdzgH7sqBZszEaU7Dkh9SIWe-L1NOMRSc0zRyHD13U874PYxIgQ-BqMYcnVseqo5QGrlb8ryU4Dn6GvG6dKopLNztptGXyR3mQC_Ys1gnhpfn94p9-_jh_uZTc_fl9vPN9V3jlG1147wSwQMYC4Co_RZjEEraKC1Yp6Nqe62skn0PsTdOt1FulTWtjVpsfHXgir099a23_FwCzd2Q6HgcjmFaqNvIrVRa2AquT6ArE1EJsduXNGA5dCC6o_tdPaR7dL8WvD53Xvoh-Ef8ZHfV35x1pGp5LDi6RH8xA7YVxlSsPWG_Ug6H_wztrr_e_7PCH7Y_oQQ</recordid><startdate>199203</startdate><enddate>199203</enddate><creator>Mitchell, Peter G.</creator><creator>Struve, Janine A.</creator><creator>Mccarthy, Geraldine M.</creator><creator>Cheung, Herman S.</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199203</creationdate><title>Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes</title><author>Mitchell, Peter G. ; Struve, Janine A. ; Mccarthy, Geraldine M. ; Cheung, Herman S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3694-cd30ed115611aa4d8afe0326f2616c4f39b43632bb1fb5c49f2836596f407d023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>Calcium Phosphates - pharmacology</topic><topic>Cartilage, Articular - cytology</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell Division - drug effects</topic><topic>Cell physiology</topic><topic>Crystallization</topic><topic>DNA - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Membrane Proteins - metabolism</topic><topic>Microbial Collagenase - genetics</topic><topic>Molecular and cellular biology</topic><topic>Protein Kinase C - pharmacology</topic><topic>Proto-Oncogene Proteins c-fos - genetics</topic><topic>Proto-Oncogene Proteins c-jun - genetics</topic><topic>RNA, Messenger</topic><topic>Transcription, Genetic</topic><toplevel>online_resources</toplevel><creatorcontrib>Mitchell, Peter G.</creatorcontrib><creatorcontrib>Struve, Janine A.</creatorcontrib><creatorcontrib>Mccarthy, Geraldine M.</creatorcontrib><creatorcontrib>Cheung, Herman S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Arthritis and rheumatism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitchell, Peter G.</au><au>Struve, Janine A.</au><au>Mccarthy, Geraldine M.</au><au>Cheung, Herman S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes</atitle><jtitle>Arthritis and rheumatism</jtitle><addtitle>Arthritis Rheum</addtitle><date>1992-03</date><risdate>1992</risdate><volume>35</volume><issue>3</issue><spage>343</spage><epage>350</epage><pages>343-350</pages><issn>0004-3591</issn><eissn>1529-0131</eissn><coden>ARHEAW</coden><abstract>Objective. To investigate BCP (basic calcium phosphate) crystal—stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes.
Methods. The role of protein kinases in BCP crystal—stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c‐fos and c‐jun message.
Results. BCP crystals stimulated chondrocyte proliferation in a PKC‐dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c‐fos, but not c‐jun.
Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>1311178</pmid><doi>10.1002/art.1780350314</doi><tpages>8</tpages></addata></record> |
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| ispartof | Arthritis and rheumatism, 1992-03, Vol.35 (3), p.343-350 |
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| subjects | Biological and medical sciences Calcium Phosphates - pharmacology Cartilage, Articular - cytology Cell cycle, cell proliferation Cell Division - drug effects Cell physiology Crystallization DNA - biosynthesis Fundamental and applied biological sciences. Psychology Humans Membrane Proteins - metabolism Microbial Collagenase - genetics Molecular and cellular biology Protein Kinase C - pharmacology Proto-Oncogene Proteins c-fos - genetics Proto-Oncogene Proteins c-jun - genetics RNA, Messenger Transcription, Genetic |
| title | Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes |
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