Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (Swissprot: accession P83003)
In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is highly immunogenic in humans. Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2002-12, Vol.67 (6), p.636-639 |
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| container_issue | 6 |
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| container_title | The American journal of tropical medicine and hygiene |
| container_volume | 67 |
| creator | Gonzalez, E Rico, G Mendoza, G Ramos, F Garcia, G Moran, P Valadez, A Melendro, EI Ximenez, C |
| description | In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is highly immunogenic in humans. Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%). |
| doi_str_mv | 10.4269/ajtmh.2002.67.636 |
| format | Article |
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Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%).</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.2002.67.636</identifier><identifier>PMID: 12518855</identifier><identifier>CODEN: AJTHAB</identifier><language>eng</language><publisher>Lawrence, KS: ASTMH</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Protozoan - blood ; Biochemistry. Physiology. Immunology. 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Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%).</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Calreticulin - chemistry</subject><subject>Calreticulin - immunology</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Entamoeba histolytica - growth & development</subject><subject>Entamoeba histolytica - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. 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| ispartof | The American journal of tropical medicine and hygiene, 2002-12, Vol.67 (6), p.636-639 |
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| subjects | Amino Acid Sequence Animals Antibodies, Protozoan - blood Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Blotting, Western Calreticulin - chemistry Calreticulin - immunology Electrophoresis, Polyacrylamide Gel - methods Entamoeba histolytica - growth & development Entamoeba histolytica - metabolism Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Humans Molecular Sequence Data Protozoa Protozoan Proteins - chemistry Protozoan Proteins - immunology Sequence Analysis, Protein |
| title | Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (Swissprot: accession P83003) |
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