Metabolite Analysis, Isolation and Purity Assessment Using Various Liquid Chromatographic Techniques Combined With Radioactivity Detection
During the discovery of metabolic routes of a drug candidate, radioactively labeled substances are administered. This study reports the multidimensional application of over-pressured layer chromatography (OPLC) and high-performance liquid chromatography (HPLC) coupled with online or off-line nondest...
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| Veröffentlicht in: | Journal of chromatographic science 2002-11, Vol.40 (10), p.603-608 |
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| container_title | Journal of chromatographic science |
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| creator | Morovján, Gy Dalmadi-Kiss, B. Klebovich, I. Mincsovics, E. |
| description | During the discovery of metabolic routes of a drug candidate, radioactively labeled substances are administered. This study reports the multidimensional application of over-pressured layer chromatography (OPLC) and high-performance liquid chromatography (HPLC) coupled with online or off-line nondestructive radioactivity detection methods in metabolism studies. Among these methods, digital autoradiography and flow-cell radioactivity detectors (RD) using solid scintillators are used. In this study, the hyphenation of OPLC with RD is reported. The application of the OPLC-RD technique is demonstrated on a metabolism study as well as the multidimensional chromatographic selectivity using normal-phase OPLC for the separation in the first dimension, followed by reversed-phase HPLC-RD, which provide additional selectivity to the separation. Information regarding the identity of radiolabeled metabolites and data obtained from spectroscopic methods could be advantageously used during structure elucidation. |
| doi_str_mv | 10.1093/chromsci/40.10.603 |
| format | Article |
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This study reports the multidimensional application of over-pressured layer chromatography (OPLC) and high-performance liquid chromatography (HPLC) coupled with online or off-line nondestructive radioactivity detection methods in metabolism studies. Among these methods, digital autoradiography and flow-cell radioactivity detectors (RD) using solid scintillators are used. In this study, the hyphenation of OPLC with RD is reported. The application of the OPLC-RD technique is demonstrated on a metabolism study as well as the multidimensional chromatographic selectivity using normal-phase OPLC for the separation in the first dimension, followed by reversed-phase HPLC-RD, which provide additional selectivity to the separation. Information regarding the identity of radiolabeled metabolites and data obtained from spectroscopic methods could be advantageously used during structure elucidation.</description><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Pharmaceutical Preparations - isolation & purification</subject><subject>Pharmaceutical Preparations - metabolism</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Radiometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morovján, Gy</creatorcontrib><creatorcontrib>Dalmadi-Kiss, B.</creatorcontrib><creatorcontrib>Klebovich, I.</creatorcontrib><creatorcontrib>Mincsovics, E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morovján, Gy</au><au>Dalmadi-Kiss, B.</au><au>Klebovich, I.</au><au>Mincsovics, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolite Analysis, Isolation and Purity Assessment Using Various Liquid Chromatographic Techniques Combined With Radioactivity Detection</atitle><jtitle>Journal of chromatographic science</jtitle><stitle>Journal of Chromatographic Science</stitle><addtitle>Journal of Chromatographic Science</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>40</volume><issue>10</issue><spage>603</spage><epage>608</epage><pages>603-608</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><coden>JCHSBZ</coden><abstract>During the discovery of metabolic routes of a drug candidate, radioactively labeled substances are administered. 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| ispartof | Journal of chromatographic science, 2002-11, Vol.40 (10), p.603-608 |
| issn | 0021-9665 1945-239X |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Free Full-Text Journals in Chemistry |
| subjects | Analysis Biological and medical sciences Chromatography, High Pressure Liquid - methods General pharmacology Medical sciences Pharmaceutical Preparations - isolation & purification Pharmaceutical Preparations - metabolism Pharmacology. Drug treatments Radiometry - methods |
| title | Metabolite Analysis, Isolation and Purity Assessment Using Various Liquid Chromatographic Techniques Combined With Radioactivity Detection |
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