Increased drug resistance of cultured human cancer cell lines in three-dimensional cellular growth assay using collagen gel matrix
We have conducted a three‐dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three‐dimensionally growing cells in collagen gel matrix we...
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| Veröffentlicht in: | Journal of surgical oncology 1992-02, Vol.49 (2), p.86-92 |
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| container_title | Journal of surgical oncology |
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| creator | Furukawa, Toshiharu Kubota, Tetsuro Watanabe, Masahiko Kase, Suguru Saikawa, Yoshiro Nishibori, Hideki Kuo, Tsong-Hong Kodaira, Susumu Ishibiki, Kyuya Kitajima, Masaki |
| description | We have conducted a three‐dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three‐dimensionally growing cells in collagen gel matrix were 2‐ to 180‐fold more resistant to mitomycin C, doxorubicin, 5‐fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three‐dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three‐dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells. |
| doi_str_mv | 10.1002/jso.2930490205 |
| format | Article |
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Three‐dimensionally growing cells in collagen gel matrix were 2‐ to 180‐fold more resistant to mitomycin C, doxorubicin, 5‐fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three‐dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three‐dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.</description><identifier>ISSN: 0022-4790</identifier><identifier>EISSN: 1096-9098</identifier><identifier>DOI: 10.1002/jso.2930490205</identifier><identifier>PMID: 1738241</identifier><identifier>CODEN: JSONAU</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - drug therapy ; Adenocarcinoma - pathology ; Antineoplastic agents ; Antineoplastic Agents - pharmacology ; Biological and medical sciences ; chemosensitivity test ; Cisplatin - pharmacology ; Collagen ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - pathology ; Culture Media - chemistry ; Doxorubicin - pharmacology ; Drug Resistance ; Drug Screening Assays, Antitumor ; fibroblasts ; Fluorouracil - pharmacology ; General aspects ; Humans ; Medical sciences ; Mitomycin - pharmacology ; MTT assay ; Pharmacology. Drug treatments ; Stomach Neoplasms - drug therapy ; Stomach Neoplasms - pathology ; Tetrazolium Salts ; Thiazoles ; Tumor Cells, Cultured</subject><ispartof>Journal of surgical oncology, 1992-02, Vol.49 (2), p.86-92</ispartof><rights>Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3885-9b8ea1108e533ec530485345579b0c81aa00ced59530d37e0f6a12ea5806ab153</citedby><cites>FETCH-LOGICAL-c3885-9b8ea1108e533ec530485345579b0c81aa00ced59530d37e0f6a12ea5806ab153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjso.2930490205$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjso.2930490205$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5210840$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1738241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furukawa, Toshiharu</creatorcontrib><creatorcontrib>Kubota, Tetsuro</creatorcontrib><creatorcontrib>Watanabe, Masahiko</creatorcontrib><creatorcontrib>Kase, Suguru</creatorcontrib><creatorcontrib>Saikawa, Yoshiro</creatorcontrib><creatorcontrib>Nishibori, Hideki</creatorcontrib><creatorcontrib>Kuo, Tsong-Hong</creatorcontrib><creatorcontrib>Kodaira, Susumu</creatorcontrib><creatorcontrib>Ishibiki, Kyuya</creatorcontrib><creatorcontrib>Kitajima, Masaki</creatorcontrib><title>Increased drug resistance of cultured human cancer cell lines in three-dimensional cellular growth assay using collagen gel matrix</title><title>Journal of surgical oncology</title><addtitle>J. Surg. Oncol</addtitle><description>We have conducted a three‐dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three‐dimensionally growing cells in collagen gel matrix were 2‐ to 180‐fold more resistant to mitomycin C, doxorubicin, 5‐fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three‐dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three‐dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.</description><subject>Adenocarcinoma - drug therapy</subject><subject>Adenocarcinoma - pathology</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>chemosensitivity test</subject><subject>Cisplatin - pharmacology</subject><subject>Collagen</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - pathology</subject><subject>Culture Media - chemistry</subject><subject>Doxorubicin - pharmacology</subject><subject>Drug Resistance</subject><subject>Drug Screening Assays, Antitumor</subject><subject>fibroblasts</subject><subject>Fluorouracil - pharmacology</subject><subject>General aspects</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mitomycin - pharmacology</subject><subject>MTT assay</subject><subject>Pharmacology. Drug treatments</subject><subject>Stomach Neoplasms - drug therapy</subject><subject>Stomach Neoplasms - pathology</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Tumor Cells, Cultured</subject><issn>0022-4790</issn><issn>1096-9098</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxSMEKkvhyg3JB8QtyziJN_YRVaUUVVutKOJozTqTrIvjFDtRu1f-crxk1YoTp5H8fm8-nrPsLYclByg-3sZhWagSKgUFiGfZgoNa5QqUfJ4tElDkVa3gZfYqxlsAUGpVnWQnvC5lUfFF9vvSm0AYqWFNmDoWKNo4ojfEhpaZyY1TSNpu6tEzc3gPzJBzzFlPkVnPxl0gyhvbk4928Oj-6pPDwLow3I87hjHink3R-o6ZwTnsyLOOHOtxDPbhdfaiRRfpzbGeZt8_n9-cfcmvri8uzz5d5aaUUuRqKwk5B0miLMmIdLIUZSVErbZgJEcEMNQIlZSmrAnaFfKCUEhY4ZaL8jT7MPe9C8OvieKoexsPu6KnYYq6LiSIQqoELmfQhCHGQK2-C7bHsNcc9CF0nULXT6Enw7tj52nbU_OEzykn_f1Rx2jQtSHlaOMjJop0VQUJUzN2bx3t_zNUf_12_c8K-exNv0cPj14MP_WqLmuhf6wv9GbNN3KzrvRN-QchwKuO</recordid><startdate>199202</startdate><enddate>199202</enddate><creator>Furukawa, Toshiharu</creator><creator>Kubota, Tetsuro</creator><creator>Watanabe, Masahiko</creator><creator>Kase, Suguru</creator><creator>Saikawa, Yoshiro</creator><creator>Nishibori, Hideki</creator><creator>Kuo, Tsong-Hong</creator><creator>Kodaira, Susumu</creator><creator>Ishibiki, Kyuya</creator><creator>Kitajima, Masaki</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199202</creationdate><title>Increased drug resistance of cultured human cancer cell lines in three-dimensional cellular growth assay using collagen gel matrix</title><author>Furukawa, Toshiharu ; Kubota, Tetsuro ; Watanabe, Masahiko ; Kase, Suguru ; Saikawa, Yoshiro ; Nishibori, Hideki ; Kuo, Tsong-Hong ; Kodaira, Susumu ; Ishibiki, Kyuya ; Kitajima, Masaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3885-9b8ea1108e533ec530485345579b0c81aa00ced59530d37e0f6a12ea5806ab153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adenocarcinoma - drug therapy</topic><topic>Adenocarcinoma - pathology</topic><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>chemosensitivity test</topic><topic>Cisplatin - pharmacology</topic><topic>Collagen</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - pathology</topic><topic>Culture Media - chemistry</topic><topic>Doxorubicin - pharmacology</topic><topic>Drug Resistance</topic><topic>Drug Screening Assays, Antitumor</topic><topic>fibroblasts</topic><topic>Fluorouracil - pharmacology</topic><topic>General aspects</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mitomycin - pharmacology</topic><topic>MTT assay</topic><topic>Pharmacology. Drug treatments</topic><topic>Stomach Neoplasms - drug therapy</topic><topic>Stomach Neoplasms - pathology</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furukawa, Toshiharu</creatorcontrib><creatorcontrib>Kubota, Tetsuro</creatorcontrib><creatorcontrib>Watanabe, Masahiko</creatorcontrib><creatorcontrib>Kase, Suguru</creatorcontrib><creatorcontrib>Saikawa, Yoshiro</creatorcontrib><creatorcontrib>Nishibori, Hideki</creatorcontrib><creatorcontrib>Kuo, Tsong-Hong</creatorcontrib><creatorcontrib>Kodaira, Susumu</creatorcontrib><creatorcontrib>Ishibiki, Kyuya</creatorcontrib><creatorcontrib>Kitajima, Masaki</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furukawa, Toshiharu</au><au>Kubota, Tetsuro</au><au>Watanabe, Masahiko</au><au>Kase, Suguru</au><au>Saikawa, Yoshiro</au><au>Nishibori, Hideki</au><au>Kuo, Tsong-Hong</au><au>Kodaira, Susumu</au><au>Ishibiki, Kyuya</au><au>Kitajima, Masaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased drug resistance of cultured human cancer cell lines in three-dimensional cellular growth assay using collagen gel matrix</atitle><jtitle>Journal of surgical oncology</jtitle><addtitle>J. Surg. Oncol</addtitle><date>1992-02</date><risdate>1992</risdate><volume>49</volume><issue>2</issue><spage>86</spage><epage>92</epage><pages>86-92</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><coden>JSONAU</coden><abstract>We have conducted a three‐dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three‐dimensionally growing cells in collagen gel matrix were 2‐ to 180‐fold more resistant to mitomycin C, doxorubicin, 5‐fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three‐dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three‐dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1738241</pmid><doi>10.1002/jso.2930490205</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of surgical oncology, 1992-02, Vol.49 (2), p.86-92 |
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| language | eng |
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| subjects | Adenocarcinoma - drug therapy Adenocarcinoma - pathology Antineoplastic agents Antineoplastic Agents - pharmacology Biological and medical sciences chemosensitivity test Cisplatin - pharmacology Collagen Colonic Neoplasms - drug therapy Colonic Neoplasms - pathology Culture Media - chemistry Doxorubicin - pharmacology Drug Resistance Drug Screening Assays, Antitumor fibroblasts Fluorouracil - pharmacology General aspects Humans Medical sciences Mitomycin - pharmacology MTT assay Pharmacology. Drug treatments Stomach Neoplasms - drug therapy Stomach Neoplasms - pathology Tetrazolium Salts Thiazoles Tumor Cells, Cultured |
| title | Increased drug resistance of cultured human cancer cell lines in three-dimensional cellular growth assay using collagen gel matrix |
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