Triacylglycerol hydrolysis in isolated hepatic endosomes
Three endosomal compartments including the compartment for uncoupling receptor and ligand (CURL), multivesicular bodies (MVB), and a putative recycling fraction (retrosomes) were isolated from rat liver homogenates fifteen minutes after a bolus injection of very low density lipoprotein (VLDL) was de...
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| Veröffentlicht in: | The Journal of biological chemistry 1992-02, Vol.267 (5), p.3396-3401 |
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| creator | Hornick, C A Thouron, C DeLamatre, J G Huang, J |
| description | Three endosomal compartments including the compartment for uncoupling receptor and ligand (CURL), multivesicular bodies (MVB),
and a putative recycling fraction (retrosomes) were isolated from rat liver homogenates fifteen minutes after a bolus injection
of very low density lipoprotein (VLDL) was delivered into a femoral vein. Assays for enzyme markers indicate a minimal contamination
with either lysosomes or Golgi. The increase in specific activity of the radiolabeled ligand (VLDL) during the isolation procedure
from homogenate to MVB, demonstrates a 200-250-fold purification of this organelle. All three fractions have the ability to
catabolize triacylglycerol substrate both as triolein and as VLDL triacylglycerol. Furthermore, incubation of isolated endosomes
following injection of endogenously labeled VLDL demonstrate their ability to hydrolyze VLDL triacylglycerol in situ. Three
distinct lipolytic pH optima were found at pH 5.5, 7.1, and 8.6. The effects of serum, MgCl2, CaCl2, NaCl, sodium dodecyl
sulfate, bile acids, and antibody to hepatic triacylglycerol lipase on the individual endosome fractions demonstrated distinct
lipolytic activities in the different compartments. Results indicate that both an endosomal neutral lipase as well as hepatic
triacylglycerol lipase make a significant contribution to lipolytic processing of endocytosed lipoproteins prior to their
resecretion of further processing in hepatic lysosomes. |
| doi_str_mv | 10.1016/S0021-9258(19)50744-X |
| format | Article |
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and a putative recycling fraction (retrosomes) were isolated from rat liver homogenates fifteen minutes after a bolus injection
of very low density lipoprotein (VLDL) was delivered into a femoral vein. Assays for enzyme markers indicate a minimal contamination
with either lysosomes or Golgi. The increase in specific activity of the radiolabeled ligand (VLDL) during the isolation procedure
from homogenate to MVB, demonstrates a 200-250-fold purification of this organelle. All three fractions have the ability to
catabolize triacylglycerol substrate both as triolein and as VLDL triacylglycerol. Furthermore, incubation of isolated endosomes
following injection of endogenously labeled VLDL demonstrate their ability to hydrolyze VLDL triacylglycerol in situ. Three
distinct lipolytic pH optima were found at pH 5.5, 7.1, and 8.6. The effects of serum, MgCl2, CaCl2, NaCl, sodium dodecyl
sulfate, bile acids, and antibody to hepatic triacylglycerol lipase on the individual endosome fractions demonstrated distinct
lipolytic activities in the different compartments. Results indicate that both an endosomal neutral lipase as well as hepatic
triacylglycerol lipase make a significant contribution to lipolytic processing of endocytosed lipoproteins prior to their
resecretion of further processing in hepatic lysosomes.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)50744-X</identifier><identifier>PMID: 1737793</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Cell Fractionation ; Centrifugation, Density Gradient ; Cholesterol Esters - metabolism ; Cholic Acid ; Cholic Acids - pharmacology ; Deoxycholic Acid - pharmacology ; Diglycerides - metabolism ; endosomes ; Fatty Acids, Nonesterified - metabolism ; Kinetics ; Lipase - isolation & purification ; Lipase - metabolism ; Lipoproteins, VLDL - metabolism ; Liver - metabolism ; Liver - ultrastructure ; Male ; Microscopy, Electron ; Oleic Acid ; Oleic Acids - metabolism ; Organelles - drug effects ; Organelles - metabolism ; Organelles - ultrastructure ; Phospholipids - metabolism ; Rats ; Rats, Inbred Strains ; triacylglycerol ; Triglycerides - metabolism</subject><ispartof>The Journal of biological chemistry, 1992-02, Vol.267 (5), p.3396-3401</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-26b5ea686667d01762ee67ae6c29bd180bf2b68f2fb8f00652773ec99fc4bd223</citedby><cites>FETCH-LOGICAL-c409t-26b5ea686667d01762ee67ae6c29bd180bf2b68f2fb8f00652773ec99fc4bd223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1737793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hornick, C A</creatorcontrib><creatorcontrib>Thouron, C</creatorcontrib><creatorcontrib>DeLamatre, J G</creatorcontrib><creatorcontrib>Huang, J</creatorcontrib><title>Triacylglycerol hydrolysis in isolated hepatic endosomes</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Three endosomal compartments including the compartment for uncoupling receptor and ligand (CURL), multivesicular bodies (MVB),
and a putative recycling fraction (retrosomes) were isolated from rat liver homogenates fifteen minutes after a bolus injection
of very low density lipoprotein (VLDL) was delivered into a femoral vein. Assays for enzyme markers indicate a minimal contamination
with either lysosomes or Golgi. The increase in specific activity of the radiolabeled ligand (VLDL) during the isolation procedure
from homogenate to MVB, demonstrates a 200-250-fold purification of this organelle. All three fractions have the ability to
catabolize triacylglycerol substrate both as triolein and as VLDL triacylglycerol. Furthermore, incubation of isolated endosomes
following injection of endogenously labeled VLDL demonstrate their ability to hydrolyze VLDL triacylglycerol in situ. Three
distinct lipolytic pH optima were found at pH 5.5, 7.1, and 8.6. The effects of serum, MgCl2, CaCl2, NaCl, sodium dodecyl
sulfate, bile acids, and antibody to hepatic triacylglycerol lipase on the individual endosome fractions demonstrated distinct
lipolytic activities in the different compartments. Results indicate that both an endosomal neutral lipase as well as hepatic
triacylglycerol lipase make a significant contribution to lipolytic processing of endocytosed lipoproteins prior to their
resecretion of further processing in hepatic lysosomes.</description><subject>Animals</subject><subject>Cell Fractionation</subject><subject>Centrifugation, Density Gradient</subject><subject>Cholesterol Esters - metabolism</subject><subject>Cholic Acid</subject><subject>Cholic Acids - pharmacology</subject><subject>Deoxycholic Acid - pharmacology</subject><subject>Diglycerides - metabolism</subject><subject>endosomes</subject><subject>Fatty Acids, Nonesterified - metabolism</subject><subject>Kinetics</subject><subject>Lipase - isolation & purification</subject><subject>Lipase - metabolism</subject><subject>Lipoproteins, VLDL - metabolism</subject><subject>Liver - metabolism</subject><subject>Liver - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Oleic Acid</subject><subject>Oleic Acids - metabolism</subject><subject>Organelles - drug effects</subject><subject>Organelles - metabolism</subject><subject>Organelles - ultrastructure</subject><subject>Phospholipids - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>triacylglycerol</subject><subject>Triglycerides - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLw0AUhQdRaq3-hEJwIbqIziOZx1KKLyi4sEJ3w8zkphlJOjXTIvn3po3o1rs5i3POvdwPoSnBtwQTfveGMSWporm8JuomxyLL0uURGhMsWcpysjxG49_IKTqL8QP3kykyQiMimBCKjZFctN64rl7VnYM21EnVFb100cfErxMfQ222UCQVbMzWuwTWRYihgXiOTkpTR7j40Ql6f3xYzJ7T-evTy-x-nroMq21Kuc3BcMk5FwUmglMALgxwR5UtiMS2pJbLkpZWlhjznArBwClVuswWlLIJuhr2btrwuYO41Y2PDurarCHsohZUYsr_ESSc9C9nqg_mQ9C1IcYWSr1pfWPaThOs92j1Aa3ec9NE6QNavex7058DO9tA8dcaWPb-5eBXflV9-Ra09cFV0GjKhc41Y4qzb434gAE</recordid><startdate>19920215</startdate><enddate>19920215</enddate><creator>Hornick, C A</creator><creator>Thouron, C</creator><creator>DeLamatre, J G</creator><creator>Huang, J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920215</creationdate><title>Triacylglycerol hydrolysis in isolated hepatic endosomes</title><author>Hornick, C A ; Thouron, C ; DeLamatre, J G ; Huang, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-26b5ea686667d01762ee67ae6c29bd180bf2b68f2fb8f00652773ec99fc4bd223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Cell Fractionation</topic><topic>Centrifugation, Density Gradient</topic><topic>Cholesterol Esters - metabolism</topic><topic>Cholic Acid</topic><topic>Cholic Acids - pharmacology</topic><topic>Deoxycholic Acid - pharmacology</topic><topic>Diglycerides - metabolism</topic><topic>endosomes</topic><topic>Fatty Acids, Nonesterified - metabolism</topic><topic>Kinetics</topic><topic>Lipase - isolation & purification</topic><topic>Lipase - metabolism</topic><topic>Lipoproteins, VLDL - metabolism</topic><topic>Liver - metabolism</topic><topic>Liver - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Oleic Acid</topic><topic>Oleic Acids - metabolism</topic><topic>Organelles - drug effects</topic><topic>Organelles - metabolism</topic><topic>Organelles - ultrastructure</topic><topic>Phospholipids - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>triacylglycerol</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hornick, C A</creatorcontrib><creatorcontrib>Thouron, C</creatorcontrib><creatorcontrib>DeLamatre, J G</creatorcontrib><creatorcontrib>Huang, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hornick, C A</au><au>Thouron, C</au><au>DeLamatre, J G</au><au>Huang, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triacylglycerol hydrolysis in isolated hepatic endosomes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1992-02-15</date><risdate>1992</risdate><volume>267</volume><issue>5</issue><spage>3396</spage><epage>3401</epage><pages>3396-3401</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Three endosomal compartments including the compartment for uncoupling receptor and ligand (CURL), multivesicular bodies (MVB),
and a putative recycling fraction (retrosomes) were isolated from rat liver homogenates fifteen minutes after a bolus injection
of very low density lipoprotein (VLDL) was delivered into a femoral vein. Assays for enzyme markers indicate a minimal contamination
with either lysosomes or Golgi. The increase in specific activity of the radiolabeled ligand (VLDL) during the isolation procedure
from homogenate to MVB, demonstrates a 200-250-fold purification of this organelle. All three fractions have the ability to
catabolize triacylglycerol substrate both as triolein and as VLDL triacylglycerol. Furthermore, incubation of isolated endosomes
following injection of endogenously labeled VLDL demonstrate their ability to hydrolyze VLDL triacylglycerol in situ. Three
distinct lipolytic pH optima were found at pH 5.5, 7.1, and 8.6. The effects of serum, MgCl2, CaCl2, NaCl, sodium dodecyl
sulfate, bile acids, and antibody to hepatic triacylglycerol lipase on the individual endosome fractions demonstrated distinct
lipolytic activities in the different compartments. Results indicate that both an endosomal neutral lipase as well as hepatic
triacylglycerol lipase make a significant contribution to lipolytic processing of endocytosed lipoproteins prior to their
resecretion of further processing in hepatic lysosomes.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1737793</pmid><doi>10.1016/S0021-9258(19)50744-X</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1992-02, Vol.267 (5), p.3396-3401 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Cell Fractionation Centrifugation, Density Gradient Cholesterol Esters - metabolism Cholic Acid Cholic Acids - pharmacology Deoxycholic Acid - pharmacology Diglycerides - metabolism endosomes Fatty Acids, Nonesterified - metabolism Kinetics Lipase - isolation & purification Lipase - metabolism Lipoproteins, VLDL - metabolism Liver - metabolism Liver - ultrastructure Male Microscopy, Electron Oleic Acid Oleic Acids - metabolism Organelles - drug effects Organelles - metabolism Organelles - ultrastructure Phospholipids - metabolism Rats Rats, Inbred Strains triacylglycerol Triglycerides - metabolism |
| title | Triacylglycerol hydrolysis in isolated hepatic endosomes |
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