A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals
Human immunodeficiency virus was detected in the serum/plasma of individuals infected with human immunodeficiency virus (HIV) after capture of virions on microparticles coated with monoclonal antibodies to external and transmembrane proteins of HIV-1. We analyzed serial samples obtained from 6 indiv...
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| Veröffentlicht in: | AIDS research and human retroviruses 1992, Vol.8 (1), p.47-52 |
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| creator | HENRARD, D. R MEHAFFEY, W. F ALLAIN, J.-P |
| description | Human immunodeficiency virus was detected in the serum/plasma of individuals infected with human immunodeficiency virus (HIV) after capture of virions on microparticles coated with monoclonal antibodies to external and transmembrane proteins of HIV-1. We analyzed serial samples obtained from 6 individuals who seroconverted, 18 asymptomatic, and 12 AIDS patients. HIV-1 RNA was detected in all (29/29) seropositive samples and in 6 seronegative samples immediately preceding seroconversion. In contrast, HIV antigen was detected in 13/29 (45%) of seropositive samples. HIV-1 RNA was also detected in 3 antigen-negative samples from one individual 8-5 months prior to seroconversion and in one sample from another person 2 days before antigen positivity. The intensity of the polymerase chain reaction (PCR) signal paralleled the concentration of HIV antigen. Conversely, seropositive HIV antigen-negative samples gave a weaker PCR signal. HIV-1 RNA was detected in 10/18 (60%) samples from asymptomatic, HIV antigen-negative, individuals and in 11/12 (92%) specimens obtained from AIDS patients. The viral capture method may provide a sensitive, specific, and semiquantitative means of detecting circulating HIV at all stages of infection. |
| doi_str_mv | 10.1089/aid.1992.8.47 |
| format | Article |
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Conversely, seropositive HIV antigen-negative samples gave a weaker PCR signal. HIV-1 RNA was detected in 10/18 (60%) samples from asymptomatic, HIV antigen-negative, individuals and in 11/12 (92%) specimens obtained from AIDS patients. 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HIV-1 RNA was also detected in 3 antigen-negative samples from one individual 8-5 months prior to seroconversion and in one sample from another person 2 days before antigen positivity. The intensity of the polymerase chain reaction (PCR) signal paralleled the concentration of HIV antigen. Conversely, seropositive HIV antigen-negative samples gave a weaker PCR signal. HIV-1 RNA was detected in 10/18 (60%) samples from asymptomatic, HIV antigen-negative, individuals and in 11/12 (92%) specimens obtained from AIDS patients. The viral capture method may provide a sensitive, specific, and semiquantitative means of detecting circulating HIV at all stages of infection.</description><subject>Acquired Immunodeficiency Syndrome - blood</subject><subject>Acquired Immunodeficiency Syndrome - microbiology</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Gene Amplification</subject><subject>HIV Core Protein p24 - analysis</subject><subject>HIV Core Protein p24 - immunology</subject><subject>HIV Reverse Transcriptase</subject><subject>HIV-1 - genetics</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Nucleic Acid Hybridization</subject><subject>Polymerase Chain Reaction</subject><subject>Prospective Studies</subject><subject>RNA, Viral - blood</subject><subject>RNA-Directed DNA Polymerase - analysis</subject><subject>Viremia - blood</subject><subject>Viremia - diagnosis</subject><subject>Virion - genetics</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0L9rGzEUwHERGlInzdixoKFkO1fSSSdpDKaNDYYsTZYO4p30BCr3w5XuDPnvc8amHTs9Hu_DG76EfOZszZmx3yCFNbdWrM1a6iuy4rbmlZFMfSArZoythBD2I7kt5TdjzAqhbsgNrzVnSq7Ir0dacChpSkekx5Shox4O05yRQinwRuOYacAJ_ZTGgY6RHjooPZws9gloGuh291qlIS4Ew7KHdExhhq58ItdxGXh_mXfk5cf3n5tttX9-2m0e95WvpZkqFW1spFXWcEDEprWhbRj3IbaKtUG1ymgfvZZt5JJ5gQC6QR0aL2MIEes78nD-e8jjnxnL5PpUPHYdDDjOxWlhmFiy_BfyhkuhpVpgdYY-j6VkjO6QUw_5zXHmTtXdUt2dqjvjpF78l8vjue0x_NPnzMv96-UOxUMXMww-lb9MKW6kqOt34t-MEw</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>HENRARD, D. 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F ; ALLAIN, J.-P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-5f9f6495981aeee6b9db601cdfb50bd5b587cfc74bf140c2eaa76e7d6c4fddfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acquired Immunodeficiency Syndrome - blood</topic><topic>Acquired Immunodeficiency Syndrome - microbiology</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Gene Amplification</topic><topic>HIV Core Protein p24 - analysis</topic><topic>HIV Core Protein p24 - immunology</topic><topic>HIV Reverse Transcriptase</topic><topic>HIV-1 - genetics</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Nucleic Acid Hybridization</topic><topic>Polymerase Chain Reaction</topic><topic>Prospective Studies</topic><topic>RNA, Viral - blood</topic><topic>RNA-Directed DNA Polymerase - analysis</topic><topic>Viremia - blood</topic><topic>Viremia - diagnosis</topic><topic>Virion - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HENRARD, D. R</creatorcontrib><creatorcontrib>MEHAFFEY, W. F</creatorcontrib><creatorcontrib>ALLAIN, J.-P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS research and human retroviruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HENRARD, D. R</au><au>MEHAFFEY, W. F</au><au>ALLAIN, J.-P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>1992</date><risdate>1992</risdate><volume>8</volume><issue>1</issue><spage>47</spage><epage>52</epage><pages>47-52</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>Human immunodeficiency virus was detected in the serum/plasma of individuals infected with human immunodeficiency virus (HIV) after capture of virions on microparticles coated with monoclonal antibodies to external and transmembrane proteins of HIV-1. We analyzed serial samples obtained from 6 individuals who seroconverted, 18 asymptomatic, and 12 AIDS patients. HIV-1 RNA was detected in all (29/29) seropositive samples and in 6 seronegative samples immediately preceding seroconversion. In contrast, HIV antigen was detected in 13/29 (45%) of seropositive samples. HIV-1 RNA was also detected in 3 antigen-negative samples from one individual 8-5 months prior to seroconversion and in one sample from another person 2 days before antigen positivity. The intensity of the polymerase chain reaction (PCR) signal paralleled the concentration of HIV antigen. Conversely, seropositive HIV antigen-negative samples gave a weaker PCR signal. HIV-1 RNA was detected in 10/18 (60%) samples from asymptomatic, HIV antigen-negative, individuals and in 11/12 (92%) specimens obtained from AIDS patients. The viral capture method may provide a sensitive, specific, and semiquantitative means of detecting circulating HIV at all stages of infection.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>1371054</pmid><doi>10.1089/aid.1992.8.47</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0889-2229 |
| ispartof | AIDS research and human retroviruses, 1992, Vol.8 (1), p.47-52 |
| issn | 0889-2229 1931-8405 |
| language | eng |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Acquired Immunodeficiency Syndrome - blood Acquired Immunodeficiency Syndrome - microbiology AIDS/HIV Biological and medical sciences Gene Amplification HIV Core Protein p24 - analysis HIV Core Protein p24 - immunology HIV Reverse Transcriptase HIV-1 - genetics Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Medical sciences Nucleic Acid Hybridization Polymerase Chain Reaction Prospective Studies RNA, Viral - blood RNA-Directed DNA Polymerase - analysis Viremia - blood Viremia - diagnosis Virion - genetics |
| title | A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals |
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