Detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyrA
Two plasmids containing the cloned Escherichia coli wild-type gyrA gene were used to transform ciprofloxacin-resistant Gram-negative clinical isolates to screen for DNA gyrase A-mediated quinolone resistance. The results show that the technique is simple and applicable to a wide range of Gram-negati...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 1992, Vol.29 (1), p.9-17 |
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| container_title | Journal of antimicrobial chemotherapy |
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| creator | POWER, E. G. M MUNOZ BELLIDO, J. L PHILLIPS, I |
| description | Two plasmids containing the cloned Escherichia coli wild-type gyrA gene were used to transform ciprofloxacin-resistant Gram-negative clinical isolates to screen for DNA gyrase A-mediated quinolone resistance. The results show that the technique is simple and applicable to a wide range of Gram-negative species including E. coli, Enterobacter cloacae, Klebsiella aerogenes, Morganella morganii, Proteus mirabilis, Pseudomonas aeruginosa, Campylobacter jejuni and Neisseria gonorrhoeae. The use of an arithmetical MIC series of dilutions (as opposed to standard geometrical ones) was found to be essential during screening for the detection of altered gyrase A. The observations were consistent with the suggestion that DNA gyrase is highly conserved among different species of bacteria and that gyrase A-mediated resistance can occur in all. |
| doi_str_mv | 10.1093/jac/29.1.9 |
| format | Article |
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G. M ; MUNOZ BELLIDO, J. L ; PHILLIPS, I</creator><creatorcontrib>POWER, E. G. M ; MUNOZ BELLIDO, J. L ; PHILLIPS, I</creatorcontrib><description>Two plasmids containing the cloned Escherichia coli wild-type gyrA gene were used to transform ciprofloxacin-resistant Gram-negative clinical isolates to screen for DNA gyrase A-mediated quinolone resistance. The results show that the technique is simple and applicable to a wide range of Gram-negative species including E. coli, Enterobacter cloacae, Klebsiella aerogenes, Morganella morganii, Proteus mirabilis, Pseudomonas aeruginosa, Campylobacter jejuni and Neisseria gonorrhoeae. The use of an arithmetical MIC series of dilutions (as opposed to standard geometrical ones) was found to be essential during screening for the detection of altered gyrase A. The observations were consistent with the suggestion that DNA gyrase is highly conserved among different species of bacteria and that gyrase A-mediated resistance can occur in all.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/29.1.9</identifier><identifier>PMID: 1310671</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Bacteriology ; Biological and medical sciences ; Ciprofloxacin - pharmacology ; DNA Topoisomerases, Type II - genetics ; Drug Resistance, Microbial - genetics ; Escherichia coli - genetics ; Fundamental and applied biological sciences. 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L</creatorcontrib><creatorcontrib>PHILLIPS, I</creatorcontrib><title>Detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyrA</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Two plasmids containing the cloned Escherichia coli wild-type gyrA gene were used to transform ciprofloxacin-resistant Gram-negative clinical isolates to screen for DNA gyrase A-mediated quinolone resistance. The results show that the technique is simple and applicable to a wide range of Gram-negative species including E. coli, Enterobacter cloacae, Klebsiella aerogenes, Morganella morganii, Proteus mirabilis, Pseudomonas aeruginosa, Campylobacter jejuni and Neisseria gonorrhoeae. The use of an arithmetical MIC series of dilutions (as opposed to standard geometrical ones) was found to be essential during screening for the detection of altered gyrase A. The observations were consistent with the suggestion that DNA gyrase is highly conserved among different species of bacteria and that gyrase A-mediated resistance can occur in all.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Ciprofloxacin - pharmacology</subject><subject>DNA Topoisomerases, Type II - genetics</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Microbiology</subject><subject>Plasmids</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAUxC0EKqWwsCNlQGxp34sdOx6r8ilVYoGFJXpxnMpVmpTYQfS_J0DEynQ63U-n0zF2iTBH0HyxJbNI9Bzn-ohNUUiIE9B4zKbAIY2VSPkpO_N-CwAyldmETZAjSIVT9nZrgzXBtU3UVpFx-66t6vaTjGuiznrnAzXGRoPbdLSLG7uh4D5sVJAJtnMUlb2NQhtRPVj67vE_8KFbnrOTimpvL0adsdf7u5fVY7x-fnhaLdfxHnUW4swkCqWViutCQYWFKAm4pFJAIaASSQJoisIWOs1I6kxjkiUktDVKVJlCPmM3v73D9vfe-pDvnDe2rqmxbe9zlSitOep_QZQCB1IN4NUI9sXOlvm-czvqDvn42pBfjzl5Q3XVDR85_4eloDJQkn8BOwl6Ug</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>POWER, E. G. M</creator><creator>MUNOZ BELLIDO, J. 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L ; PHILLIPS, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p198t-8c2716e6739b70f1b4da036ad40b40f42201cbbeb958a69891282a49ec74f8713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Ciprofloxacin - pharmacology</topic><topic>DNA Topoisomerases, Type II - genetics</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Gram-Negative Bacteria - drug effects</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Microbiology</topic><topic>Plasmids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>POWER, E. G. M</creatorcontrib><creatorcontrib>MUNOZ BELLIDO, J. L</creatorcontrib><creatorcontrib>PHILLIPS, I</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>POWER, E. G. M</au><au>MUNOZ BELLIDO, J. L</au><au>PHILLIPS, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyrA</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>1992</date><risdate>1992</risdate><volume>29</volume><issue>1</issue><spage>9</spage><epage>17</epage><pages>9-17</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Two plasmids containing the cloned Escherichia coli wild-type gyrA gene were used to transform ciprofloxacin-resistant Gram-negative clinical isolates to screen for DNA gyrase A-mediated quinolone resistance. The results show that the technique is simple and applicable to a wide range of Gram-negative species including E. coli, Enterobacter cloacae, Klebsiella aerogenes, Morganella morganii, Proteus mirabilis, Pseudomonas aeruginosa, Campylobacter jejuni and Neisseria gonorrhoeae. The use of an arithmetical MIC series of dilutions (as opposed to standard geometrical ones) was found to be essential during screening for the detection of altered gyrase A. The observations were consistent with the suggestion that DNA gyrase is highly conserved among different species of bacteria and that gyrase A-mediated resistance can occur in all.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1310671</pmid><doi>10.1093/jac/29.1.9</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-7453 |
| ispartof | Journal of antimicrobial chemotherapy, 1992, Vol.29 (1), p.9-17 |
| issn | 0305-7453 1460-2091 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Bacteriology Biological and medical sciences Ciprofloxacin - pharmacology DNA Topoisomerases, Type II - genetics Drug Resistance, Microbial - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetics Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - genetics Microbiology Plasmids |
| title | Detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyrA |
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