Expression of a monocyte chemotactic cytokine by human mononuclear phagocytes
The present study was designed to investigate the capacity of human mononuclear phagocytes to produce a cytokine chemotactic for monocytes (monocyte chemotactic protein (MCP), alternative acronyms JE, monocyte chemotactic and activating factor, MCP-1, and tumor-derived chemotactic factor). Human PBM...
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| Veröffentlicht in: | The Journal of immunology (1950) 1992-02, Vol.148 (3), p.760-765 |
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| container_title | The Journal of immunology (1950) |
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| creator | Colotta, F Borre, A Wang, JM Tattanelli, M Maddalena, F Polentarutti, N Peri, G Mantovani, A |
| description | The present study was designed to investigate the capacity of human mononuclear phagocytes to produce a cytokine chemotactic for monocytes (monocyte chemotactic protein (MCP), alternative acronyms JE, monocyte chemotactic and activating factor, MCP-1, and tumor-derived chemotactic factor). Human PBMC exposed in vitro to bacterial LPS expressed high levels of MCP transcripts. Monocyte-depleted lymphoid cells were not induced to express MCP by LPS. Percoll-gradient purified monocytes were able to express high levels of MCP transcripts. In an effort to exclude a role of contaminating non-monocytic cells, mononuclear phagocytes were separated by flow cytometry and sorting: CD14+ cells exposed to LPS showed high levels of MCP mRNA. LPS-stimulated monocytes released chemotactic activity for monocytes that could be inhibited by absorption with anti-MCP antibodies. IL-1, TNF, IFN-gamma, granulocyte-macrophage-CSF and, to a lesser extent, macrophage-CSF, as well as inactivated streptococci, also induced MCP gene expression. Actinomycin D experiments indicated that induction of MCP in monocytes was gene transcription-dependent. The protein synthesis inhibitor cycloheximide (Cy) blocked IL-1-, TNF-, or LPS-induced MCP gene expression in monocytes. In contrast, expression of the structurally related chemotactic cytokine IL-8 was superinduced by Cy. Moreover, Cy superinduced MCP gene expression in cells other than monocytes, including endothelial cells, smooth muscle cell and fibrosarcoma cells, indicating different mechanisms of regulation in mononuclear phagocytes vs cells of other lineages. The capacity of cells of the monocyte-macrophage lineage to produce a cytokine that recruits and activates circulating monocytes may be of considerable importance in inflammatory and immunologic reactions. Thus, the mononuclear phagocyte system can autonomously regulate the extravasation and activation of immature elements of the same lineage, a key event in inflammation and immunity. |
| doi_str_mv | 10.4049/jimmunol.148.3.760 |
| format | Article |
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Human PBMC exposed in vitro to bacterial LPS expressed high levels of MCP transcripts. Monocyte-depleted lymphoid cells were not induced to express MCP by LPS. Percoll-gradient purified monocytes were able to express high levels of MCP transcripts. In an effort to exclude a role of contaminating non-monocytic cells, mononuclear phagocytes were separated by flow cytometry and sorting: CD14+ cells exposed to LPS showed high levels of MCP mRNA. LPS-stimulated monocytes released chemotactic activity for monocytes that could be inhibited by absorption with anti-MCP antibodies. IL-1, TNF, IFN-gamma, granulocyte-macrophage-CSF and, to a lesser extent, macrophage-CSF, as well as inactivated streptococci, also induced MCP gene expression. Actinomycin D experiments indicated that induction of MCP in monocytes was gene transcription-dependent. The protein synthesis inhibitor cycloheximide (Cy) blocked IL-1-, TNF-, or LPS-induced MCP gene expression in monocytes. In contrast, expression of the structurally related chemotactic cytokine IL-8 was superinduced by Cy. Moreover, Cy superinduced MCP gene expression in cells other than monocytes, including endothelial cells, smooth muscle cell and fibrosarcoma cells, indicating different mechanisms of regulation in mononuclear phagocytes vs cells of other lineages. The capacity of cells of the monocyte-macrophage lineage to produce a cytokine that recruits and activates circulating monocytes may be of considerable importance in inflammatory and immunologic reactions. Thus, the mononuclear phagocyte system can autonomously regulate the extravasation and activation of immature elements of the same lineage, a key event in inflammation and immunity.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.148.3.760</identifier><identifier>PMID: 1370516</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Antigens, CD - analysis ; Antigens, Differentiation, Myelomonocytic - analysis ; Biological and medical sciences ; Blotting, Northern ; Cells, Cultured ; Chemokine CCL2 ; Chemotactic Factors - genetics ; Chemotactic Factors - metabolism ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Expression - drug effects ; Humans ; Immunobiology ; In Vitro Techniques ; Leukocytes, Mononuclear - metabolism ; Lipopolysaccharide Receptors ; Macrophages - metabolism ; Miscellaneous ; Monocytes - metabolism ; Phagocytes - metabolism ; Regulatory factors and their cellular receptors ; RNA, Messenger - genetics</subject><ispartof>The Journal of immunology (1950), 1992-02, Vol.148 (3), p.760-765</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-8e1d9a3f846aac0618d53bb46204a1697a3265d5a9b214ab5c50563f5c0021a13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5109916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1370516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Colotta, F</creatorcontrib><creatorcontrib>Borre, A</creatorcontrib><creatorcontrib>Wang, JM</creatorcontrib><creatorcontrib>Tattanelli, M</creatorcontrib><creatorcontrib>Maddalena, F</creatorcontrib><creatorcontrib>Polentarutti, N</creatorcontrib><creatorcontrib>Peri, G</creatorcontrib><creatorcontrib>Mantovani, A</creatorcontrib><title>Expression of a monocyte chemotactic cytokine by human mononuclear phagocytes</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>The present study was designed to investigate the capacity of human mononuclear phagocytes to produce a cytokine chemotactic for monocytes (monocyte chemotactic protein (MCP), alternative acronyms JE, monocyte chemotactic and activating factor, MCP-1, and tumor-derived chemotactic factor). Human PBMC exposed in vitro to bacterial LPS expressed high levels of MCP transcripts. Monocyte-depleted lymphoid cells were not induced to express MCP by LPS. Percoll-gradient purified monocytes were able to express high levels of MCP transcripts. In an effort to exclude a role of contaminating non-monocytic cells, mononuclear phagocytes were separated by flow cytometry and sorting: CD14+ cells exposed to LPS showed high levels of MCP mRNA. LPS-stimulated monocytes released chemotactic activity for monocytes that could be inhibited by absorption with anti-MCP antibodies. IL-1, TNF, IFN-gamma, granulocyte-macrophage-CSF and, to a lesser extent, macrophage-CSF, as well as inactivated streptococci, also induced MCP gene expression. Actinomycin D experiments indicated that induction of MCP in monocytes was gene transcription-dependent. The protein synthesis inhibitor cycloheximide (Cy) blocked IL-1-, TNF-, or LPS-induced MCP gene expression in monocytes. In contrast, expression of the structurally related chemotactic cytokine IL-8 was superinduced by Cy. Moreover, Cy superinduced MCP gene expression in cells other than monocytes, including endothelial cells, smooth muscle cell and fibrosarcoma cells, indicating different mechanisms of regulation in mononuclear phagocytes vs cells of other lineages. The capacity of cells of the monocyte-macrophage lineage to produce a cytokine that recruits and activates circulating monocytes may be of considerable importance in inflammatory and immunologic reactions. Thus, the mononuclear phagocyte system can autonomously regulate the extravasation and activation of immature elements of the same lineage, a key event in inflammation and immunity.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, Differentiation, Myelomonocytic - analysis</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cells, Cultured</subject><subject>Chemokine CCL2</subject><subject>Chemotactic Factors - genetics</subject><subject>Chemotactic Factors - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Expression - drug effects</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>In Vitro Techniques</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lipopolysaccharide Receptors</subject><subject>Macrophages - metabolism</subject><subject>Miscellaneous</subject><subject>Monocytes - metabolism</subject><subject>Phagocytes - metabolism</subject><subject>Regulatory factors and their cellular receptors</subject><subject>RNA, Messenger - genetics</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi1UVLaFF0BCygFxyzLj2M76iKqFVmrFBc7WxOs0LnG82ImWffumu0t77MmS5_t_ez7GPiIsBQj99cGHMA2xX6JYLatlreANW6CUUCoF6owtADgvsVb1O3aR8wMAKODinJ1jVYNEtWB363_b5HL2cShiW1AR4hDtfnSF7VyII9nR22K-iH_84IpmX3RToOGADZPtHaVi29H9IZPfs7ct9dl9OJ2X7Pf39a-r6_L254-bq2-3pRVaj-XK4UZT1a6EIrKgcLWRVdMIxUEQKl1TxZXcSNINR0GNtBKkqlpp54WQsLpkX4692xT_Ti6PJvhsXd_T4OKUTc1rPS8oXwVRIQjBnxr5EbQp5pxca7bJB0p7g2CeZJv_ss0s21Rmlj2HPp3apya4zUvkaHeefz7NKVvq20SD9fkZkwhaH7DTJzt_3-18ciYH6vu5FM1ut3t57xEwaZbu</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>Colotta, F</creator><creator>Borre, A</creator><creator>Wang, JM</creator><creator>Tattanelli, M</creator><creator>Maddalena, F</creator><creator>Polentarutti, N</creator><creator>Peri, G</creator><creator>Mantovani, A</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19920201</creationdate><title>Expression of a monocyte chemotactic cytokine by human mononuclear phagocytes</title><author>Colotta, F ; Borre, A ; Wang, JM ; Tattanelli, M ; Maddalena, F ; Polentarutti, N ; Peri, G ; Mantovani, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-8e1d9a3f846aac0618d53bb46204a1697a3265d5a9b214ab5c50563f5c0021a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, Differentiation, Myelomonocytic - analysis</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cells, Cultured</topic><topic>Chemokine CCL2</topic><topic>Chemotactic Factors - genetics</topic><topic>Chemotactic Factors - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Expression - drug effects</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>In Vitro Techniques</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Lipopolysaccharide Receptors</topic><topic>Macrophages - metabolism</topic><topic>Miscellaneous</topic><topic>Monocytes - metabolism</topic><topic>Phagocytes - metabolism</topic><topic>Regulatory factors and their cellular receptors</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Colotta, F</creatorcontrib><creatorcontrib>Borre, A</creatorcontrib><creatorcontrib>Wang, JM</creatorcontrib><creatorcontrib>Tattanelli, M</creatorcontrib><creatorcontrib>Maddalena, F</creatorcontrib><creatorcontrib>Polentarutti, N</creatorcontrib><creatorcontrib>Peri, G</creatorcontrib><creatorcontrib>Mantovani, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Colotta, F</au><au>Borre, A</au><au>Wang, JM</au><au>Tattanelli, M</au><au>Maddalena, F</au><au>Polentarutti, N</au><au>Peri, G</au><au>Mantovani, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a monocyte chemotactic cytokine by human mononuclear phagocytes</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>148</volume><issue>3</issue><spage>760</spage><epage>765</epage><pages>760-765</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>The present study was designed to investigate the capacity of human mononuclear phagocytes to produce a cytokine chemotactic for monocytes (monocyte chemotactic protein (MCP), alternative acronyms JE, monocyte chemotactic and activating factor, MCP-1, and tumor-derived chemotactic factor). Human PBMC exposed in vitro to bacterial LPS expressed high levels of MCP transcripts. Monocyte-depleted lymphoid cells were not induced to express MCP by LPS. Percoll-gradient purified monocytes were able to express high levels of MCP transcripts. In an effort to exclude a role of contaminating non-monocytic cells, mononuclear phagocytes were separated by flow cytometry and sorting: CD14+ cells exposed to LPS showed high levels of MCP mRNA. LPS-stimulated monocytes released chemotactic activity for monocytes that could be inhibited by absorption with anti-MCP antibodies. IL-1, TNF, IFN-gamma, granulocyte-macrophage-CSF and, to a lesser extent, macrophage-CSF, as well as inactivated streptococci, also induced MCP gene expression. Actinomycin D experiments indicated that induction of MCP in monocytes was gene transcription-dependent. The protein synthesis inhibitor cycloheximide (Cy) blocked IL-1-, TNF-, or LPS-induced MCP gene expression in monocytes. In contrast, expression of the structurally related chemotactic cytokine IL-8 was superinduced by Cy. Moreover, Cy superinduced MCP gene expression in cells other than monocytes, including endothelial cells, smooth muscle cell and fibrosarcoma cells, indicating different mechanisms of regulation in mononuclear phagocytes vs cells of other lineages. The capacity of cells of the monocyte-macrophage lineage to produce a cytokine that recruits and activates circulating monocytes may be of considerable importance in inflammatory and immunologic reactions. Thus, the mononuclear phagocyte system can autonomously regulate the extravasation and activation of immature elements of the same lineage, a key event in inflammation and immunity.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>1370516</pmid><doi>10.4049/jimmunol.148.3.760</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Antigens, CD - analysis Antigens, Differentiation, Myelomonocytic - analysis Biological and medical sciences Blotting, Northern Cells, Cultured Chemokine CCL2 Chemotactic Factors - genetics Chemotactic Factors - metabolism Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Expression - drug effects Humans Immunobiology In Vitro Techniques Leukocytes, Mononuclear - metabolism Lipopolysaccharide Receptors Macrophages - metabolism Miscellaneous Monocytes - metabolism Phagocytes - metabolism Regulatory factors and their cellular receptors RNA, Messenger - genetics |
| title | Expression of a monocyte chemotactic cytokine by human mononuclear phagocytes |
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