Induction of a 72-kDa heat-shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine
An antiulcer drug, zinc L-carnosine (polaprezinc), provides gastric mucosal protection against various irritants. In this study, we evaluated the effects of zinc L-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in...
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| Veröffentlicht in: | Digestive diseases and sciences 2002-12, Vol.47 (12), p.2799-2804 |
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| creator | ODASHIMA, Masaru OTAKA, Michiro JIN, Mario KONISHI, Noriaki SATO, Toshihiro KATO, Sayuri MATSUHASHI, Tamotsu NAKAMURA, Chieko WATANABE, Sumio |
| description | An antiulcer drug, zinc L-carnosine (polaprezinc), provides gastric mucosal protection against various irritants. In this study, we evaluated the effects of zinc L-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in a wide variety of cells, including rat gastric mucosa in vitro and in vivo. Expression of HSP72 after exposure to zinc L-carnosine, zinc sulfate, or L-carnosine (1-300 microM) in rat gastric mucosal cells (RGM1) and intragastric administration of zinc L-carnosine, zinc sulfate (30 or 100 mg/kg) and L-carnosine (76 mg/kg) was investigated by western blotting and densitometric analysis. Exposure to zinc L-carnosine and zinc sulfate increased the expression of HSP72 significantly in RGM1 cells. Intragastric administration of zinc L-carnosine and zinc sulfate showed significant increment in HSP72 in rat gastric mucosa also in vivo. The ability to induce HSP72 is significantly higher in zinc L-carnosine compared with zinc sulfate based on molecular concentration in vivo. However, L-carnosine did not increase the expression of HSP72 in vitro and in vivo. Zinc derivatives, especially zinc L-carnosine, could be a strong HSP72 (chaperon) inducer, which has been known to enhance mucosal protective ability. |
| doi_str_mv | 10.1023/a:1021029927386 |
| format | Article |
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In this study, we evaluated the effects of zinc L-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in a wide variety of cells, including rat gastric mucosa in vitro and in vivo. Expression of HSP72 after exposure to zinc L-carnosine, zinc sulfate, or L-carnosine (1-300 microM) in rat gastric mucosal cells (RGM1) and intragastric administration of zinc L-carnosine, zinc sulfate (30 or 100 mg/kg) and L-carnosine (76 mg/kg) was investigated by western blotting and densitometric analysis. Exposure to zinc L-carnosine and zinc sulfate increased the expression of HSP72 significantly in RGM1 cells. Intragastric administration of zinc L-carnosine and zinc sulfate showed significant increment in HSP72 in rat gastric mucosa also in vivo. The ability to induce HSP72 is significantly higher in zinc L-carnosine compared with zinc sulfate based on molecular concentration in vivo. However, L-carnosine did not increase the expression of HSP72 in vitro and in vivo. Zinc derivatives, especially zinc L-carnosine, could be a strong HSP72 (chaperon) inducer, which has been known to enhance mucosal protective ability.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1023/a:1021029927386</identifier><identifier>PMID: 12498304</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Biological and medical sciences ; Carnosine - analogs & derivatives ; Carnosine - pharmacology ; Cells, Cultured ; Digestive system ; Gastric Mucins - metabolism ; Gastric Mucosa - metabolism ; Heat-Shock Proteins - metabolism ; HSP72 Heat-Shock Proteins ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Organometallic Compounds - pharmacology ; Pathology. Cytology. Biochemistry. Spectrometry. 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In this study, we evaluated the effects of zinc L-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in a wide variety of cells, including rat gastric mucosa in vitro and in vivo. Expression of HSP72 after exposure to zinc L-carnosine, zinc sulfate, or L-carnosine (1-300 microM) in rat gastric mucosal cells (RGM1) and intragastric administration of zinc L-carnosine, zinc sulfate (30 or 100 mg/kg) and L-carnosine (76 mg/kg) was investigated by western blotting and densitometric analysis. Exposure to zinc L-carnosine and zinc sulfate increased the expression of HSP72 significantly in RGM1 cells. Intragastric administration of zinc L-carnosine and zinc sulfate showed significant increment in HSP72 in rat gastric mucosa also in vivo. The ability to induce HSP72 is significantly higher in zinc L-carnosine compared with zinc sulfate based on molecular concentration in vivo. However, L-carnosine did not increase the expression of HSP72 in vitro and in vivo. Zinc derivatives, especially zinc L-carnosine, could be a strong HSP72 (chaperon) inducer, which has been known to enhance mucosal protective ability.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carnosine - analogs & derivatives</subject><subject>Carnosine - pharmacology</subject><subject>Cells, Cultured</subject><subject>Digestive system</subject><subject>Gastric Mucins - metabolism</subject><subject>Gastric Mucosa - metabolism</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>HSP72 Heat-Shock Proteins</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Organometallic Compounds - pharmacology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Zinc</topic><topic>Zinc Compounds</topic><topic>Zinc Sulfate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ODASHIMA, Masaru</creatorcontrib><creatorcontrib>OTAKA, Michiro</creatorcontrib><creatorcontrib>JIN, Mario</creatorcontrib><creatorcontrib>KONISHI, Noriaki</creatorcontrib><creatorcontrib>SATO, Toshihiro</creatorcontrib><creatorcontrib>KATO, Sayuri</creatorcontrib><creatorcontrib>MATSUHASHI, Tamotsu</creatorcontrib><creatorcontrib>NAKAMURA, Chieko</creatorcontrib><creatorcontrib>WATANABE, Sumio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ODASHIMA, Masaru</au><au>OTAKA, Michiro</au><au>JIN, Mario</au><au>KONISHI, Noriaki</au><au>SATO, Toshihiro</au><au>KATO, Sayuri</au><au>MATSUHASHI, Tamotsu</au><au>NAKAMURA, Chieko</au><au>WATANABE, Sumio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of a 72-kDa heat-shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine</atitle><jtitle>Digestive diseases and sciences</jtitle><addtitle>Dig Dis Sci</addtitle><date>2002-12-01</date><risdate>2002</risdate><volume>47</volume><issue>12</issue><spage>2799</spage><epage>2804</epage><pages>2799-2804</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>An antiulcer drug, zinc L-carnosine (polaprezinc), provides gastric mucosal protection against various irritants. In this study, we evaluated the effects of zinc L-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in a wide variety of cells, including rat gastric mucosa in vitro and in vivo. Expression of HSP72 after exposure to zinc L-carnosine, zinc sulfate, or L-carnosine (1-300 microM) in rat gastric mucosal cells (RGM1) and intragastric administration of zinc L-carnosine, zinc sulfate (30 or 100 mg/kg) and L-carnosine (76 mg/kg) was investigated by western blotting and densitometric analysis. Exposure to zinc L-carnosine and zinc sulfate increased the expression of HSP72 significantly in RGM1 cells. Intragastric administration of zinc L-carnosine and zinc sulfate showed significant increment in HSP72 in rat gastric mucosa also in vivo. The ability to induce HSP72 is significantly higher in zinc L-carnosine compared with zinc sulfate based on molecular concentration in vivo. However, L-carnosine did not increase the expression of HSP72 in vitro and in vivo. Zinc derivatives, especially zinc L-carnosine, could be a strong HSP72 (chaperon) inducer, which has been known to enhance mucosal protective ability.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>12498304</pmid><doi>10.1023/a:1021029927386</doi><tpages>6</tpages></addata></record> |
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| ispartof | Digestive diseases and sciences, 2002-12, Vol.47 (12), p.2799-2804 |
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| subjects | Animals Biological and medical sciences Carnosine - analogs & derivatives Carnosine - pharmacology Cells, Cultured Digestive system Gastric Mucins - metabolism Gastric Mucosa - metabolism Heat-Shock Proteins - metabolism HSP72 Heat-Shock Proteins Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Organometallic Compounds - pharmacology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Rats Rats, Sprague-Dawley Zinc Zinc Compounds Zinc Sulfate - pharmacology |
| title | Induction of a 72-kDa heat-shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine |
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