Granulocyte sequestration and early failure in the autoperfused heart-lung preparation
We investigated the role of pulmonary granulocyte sequestration in the development of early failure of the autoperfused working heart-lung preparation. A significant decline in the total circulating leukocyte count in 21 preparations at 60 minutes of perfusion (5.0 to 1.4 × 10 3/μL; 28% of baseline;...
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| Veröffentlicht in: | The Annals of thoracic surgery 1992-02, Vol.53 (2), p.217-226 |
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| creator | Genco, Christopher M. Connolly, Raymond J. Peterson, Myron B. Bernstein, Eugene A. Ramberg, Karen Zhang, Xi Cleveland, Richard J. Diehl, James T. |
| description | We investigated the role of pulmonary granulocyte sequestration in the development of early failure of the autoperfused working heart-lung preparation. A significant decline in the total circulating leukocyte count in 21 preparations at 60 minutes of perfusion (5.0 to 1.4 × 10
3/μL; 28% of baseline;
p < 0.001) was observed. Differential cell counts in 14 of these preparations revealed a predominant decrease in granulocyte count (8.7% of baseline) and a moderate decline in lymphocyte count (46% of baseline). In study I, indium 111-labeled autologous granulocytes were injected intravenously into 10 adult New Zealand While rabbits. In group I (n = 5), an autoperfused working heart-lung preparation was harvested and perfused for 60 minutes. In group II (controls, n = 5), the heart-lung block was harvested following 60 minutes of in situ perfusion. Organ blocks were imaged before and after saline flush. There was a significant decline in granulocyte counts at 60 minutes of perfusion in group I versus no change in group II (I, 2.3 ± 0.4 to 0.3 ± 0.1;
p < 0.01; II, 1.7 ± 0.2 to 2.3 ± 0.5; not significant; ×10
3/μL ± standard error of the mean). Postflush lung activity was significantly increased in group I versus group II (I, 3,751 ± 566; II, 1,867 ± 532;
p < 0.05; counts ± standard error of the mean). In study II, 15 autoperfused preparations were divided into two groups. Group I (n = 10) preparations were controls. Group II (n = 5) animals were depleted of leukocytes by pretreating with nitrogen mustard. Group I (controls) produced a bimodal survival distribution (Ia, 8.2 ± 1.0) Ib, 26.4 ± 2.0; hours ± standard error of the mean). Group II survival was significantly longer than that of group Ia and similar to that of group Ib (II, 25.3 ± 2.2;
p < 0.01 versus group Ia, not significant versus group Ib; hours ± standard error of the mean). Hemodynamic profiles for group II closely paralleled those of group Ib. In conclusion, pulmonary sequestration of granulocytes occurs early in the autoperfused working heart-lung preparation (within 60 minutes of autoperfusion), and preoperative leukocyte depletion prolongs survival of the autoperfused working heart-lung preparation by eliminating the subset group Ia (short survivors) seen in untreated preparations. |
| doi_str_mv | 10.1016/0003-4975(92)91322-Z |
| format | Article |
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3/μL; 28% of baseline;
p < 0.001) was observed. Differential cell counts in 14 of these preparations revealed a predominant decrease in granulocyte count (8.7% of baseline) and a moderate decline in lymphocyte count (46% of baseline). In study I, indium 111-labeled autologous granulocytes were injected intravenously into 10 adult New Zealand While rabbits. In group I (n = 5), an autoperfused working heart-lung preparation was harvested and perfused for 60 minutes. In group II (controls, n = 5), the heart-lung block was harvested following 60 minutes of in situ perfusion. Organ blocks were imaged before and after saline flush. There was a significant decline in granulocyte counts at 60 minutes of perfusion in group I versus no change in group II (I, 2.3 ± 0.4 to 0.3 ± 0.1;
p < 0.01; II, 1.7 ± 0.2 to 2.3 ± 0.5; not significant; ×10
3/μL ± standard error of the mean). Postflush lung activity was significantly increased in group I versus group II (I, 3,751 ± 566; II, 1,867 ± 532;
p < 0.05; counts ± standard error of the mean). In study II, 15 autoperfused preparations were divided into two groups. Group I (n = 10) preparations were controls. Group II (n = 5) animals were depleted of leukocytes by pretreating with nitrogen mustard. Group I (controls) produced a bimodal survival distribution (Ia, 8.2 ± 1.0) Ib, 26.4 ± 2.0; hours ± standard error of the mean). Group II survival was significantly longer than that of group Ia and similar to that of group Ib (II, 25.3 ± 2.2;
p < 0.01 versus group Ia, not significant versus group Ib; hours ± standard error of the mean). Hemodynamic profiles for group II closely paralleled those of group Ib. In conclusion, pulmonary sequestration of granulocytes occurs early in the autoperfused working heart-lung preparation (within 60 minutes of autoperfusion), and preoperative leukocyte depletion prolongs survival of the autoperfused working heart-lung preparation by eliminating the subset group Ia (short survivors) seen in untreated preparations.</description><identifier>ISSN: 0003-4975</identifier><identifier>EISSN: 1552-6259</identifier><identifier>DOI: 10.1016/0003-4975(92)91322-Z</identifier><identifier>PMID: 1731660</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Blood Gas Analysis ; Blood Pressure ; Cardiac Output ; Granulocytes - diagnostic imaging ; Granulocytes - physiology ; Heart ; Leukocyte Count ; Lung ; Lung Compliance ; Organ Preservation - methods ; Platelet Count ; Pulmonary Artery - physiology ; Rabbits ; Radionuclide Imaging ; Tissue Survival</subject><ispartof>The Annals of thoracic surgery, 1992-02, Vol.53 (2), p.217-226</ispartof><rights>1992 The Society of Thoracic Surgeons</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-cc1c3970af35101afc0da5d553b1cb74c59f74f60ed1dedda40eb6a0af71bd9d3</citedby><cites>FETCH-LOGICAL-c458t-cc1c3970af35101afc0da5d553b1cb74c59f74f60ed1dedda40eb6a0af71bd9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1731660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Genco, Christopher M.</creatorcontrib><creatorcontrib>Connolly, Raymond J.</creatorcontrib><creatorcontrib>Peterson, Myron B.</creatorcontrib><creatorcontrib>Bernstein, Eugene A.</creatorcontrib><creatorcontrib>Ramberg, Karen</creatorcontrib><creatorcontrib>Zhang, Xi</creatorcontrib><creatorcontrib>Cleveland, Richard J.</creatorcontrib><creatorcontrib>Diehl, James T.</creatorcontrib><title>Granulocyte sequestration and early failure in the autoperfused heart-lung preparation</title><title>The Annals of thoracic surgery</title><addtitle>Ann Thorac Surg</addtitle><description>We investigated the role of pulmonary granulocyte sequestration in the development of early failure of the autoperfused working heart-lung preparation. A significant decline in the total circulating leukocyte count in 21 preparations at 60 minutes of perfusion (5.0 to 1.4 × 10
3/μL; 28% of baseline;
p < 0.001) was observed. Differential cell counts in 14 of these preparations revealed a predominant decrease in granulocyte count (8.7% of baseline) and a moderate decline in lymphocyte count (46% of baseline). In study I, indium 111-labeled autologous granulocytes were injected intravenously into 10 adult New Zealand While rabbits. In group I (n = 5), an autoperfused working heart-lung preparation was harvested and perfused for 60 minutes. In group II (controls, n = 5), the heart-lung block was harvested following 60 minutes of in situ perfusion. Organ blocks were imaged before and after saline flush. There was a significant decline in granulocyte counts at 60 minutes of perfusion in group I versus no change in group II (I, 2.3 ± 0.4 to 0.3 ± 0.1;
p < 0.01; II, 1.7 ± 0.2 to 2.3 ± 0.5; not significant; ×10
3/μL ± standard error of the mean). Postflush lung activity was significantly increased in group I versus group II (I, 3,751 ± 566; II, 1,867 ± 532;
p < 0.05; counts ± standard error of the mean). In study II, 15 autoperfused preparations were divided into two groups. Group I (n = 10) preparations were controls. Group II (n = 5) animals were depleted of leukocytes by pretreating with nitrogen mustard. Group I (controls) produced a bimodal survival distribution (Ia, 8.2 ± 1.0) Ib, 26.4 ± 2.0; hours ± standard error of the mean). Group II survival was significantly longer than that of group Ia and similar to that of group Ib (II, 25.3 ± 2.2;
p < 0.01 versus group Ia, not significant versus group Ib; hours ± standard error of the mean). Hemodynamic profiles for group II closely paralleled those of group Ib. In conclusion, pulmonary sequestration of granulocytes occurs early in the autoperfused working heart-lung preparation (within 60 minutes of autoperfusion), and preoperative leukocyte depletion prolongs survival of the autoperfused working heart-lung preparation by eliminating the subset group Ia (short survivors) seen in untreated preparations.</description><subject>Animals</subject><subject>Blood Gas Analysis</subject><subject>Blood Pressure</subject><subject>Cardiac Output</subject><subject>Granulocytes - diagnostic imaging</subject><subject>Granulocytes - physiology</subject><subject>Heart</subject><subject>Leukocyte Count</subject><subject>Lung</subject><subject>Lung Compliance</subject><subject>Organ Preservation - methods</subject><subject>Platelet Count</subject><subject>Pulmonary Artery - physiology</subject><subject>Rabbits</subject><subject>Radionuclide Imaging</subject><subject>Tissue Survival</subject><issn>0003-4975</issn><issn>1552-6259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFLwzAYhoMoc07_gUJOoodq0jTNchFk6BQGXtTDLiFNvrpI19akFfbvzdahN08hvM_7Jd-D0DklN5TQ_JYQwpJMCn4l02tJWZomywM0ppynSZ5yeYjGv8gxOgnhM17TGI_QiApG85yM0fvc67qvGrPpAAf46iF0XneuqbGuLQbtqw0utat6D9jVuFsB1n3XtODLPoDFq4h0SdXXH7j10OqhfIqOSl0FONufE_T2-PA6e0oWL_Pn2f0iMRmfdokx1DApiC4Zjzvp0hCrueWcFdQUIjNcliIrcwKWWrBWZwSKXEde0MJKyybocpjb-mb3d7V2wUBV6RqaPiiRCklYNo1gNoDGNyF4KFXr3Vr7jaJEbXWqrSu1daVkqnY61TLWLvbz-2IN9q80-Iv53ZBDXPLbgVfBOKgNWOfBdMo27v8HfgCifocd</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>Genco, Christopher M.</creator><creator>Connolly, Raymond J.</creator><creator>Peterson, Myron B.</creator><creator>Bernstein, Eugene A.</creator><creator>Ramberg, Karen</creator><creator>Zhang, Xi</creator><creator>Cleveland, Richard J.</creator><creator>Diehl, James T.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920201</creationdate><title>Granulocyte sequestration and early failure in the autoperfused heart-lung preparation</title><author>Genco, Christopher M. ; Connolly, Raymond J. ; Peterson, Myron B. ; Bernstein, Eugene A. ; Ramberg, Karen ; Zhang, Xi ; Cleveland, Richard J. ; Diehl, James T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-cc1c3970af35101afc0da5d553b1cb74c59f74f60ed1dedda40eb6a0af71bd9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Blood Gas Analysis</topic><topic>Blood Pressure</topic><topic>Cardiac Output</topic><topic>Granulocytes - diagnostic imaging</topic><topic>Granulocytes - physiology</topic><topic>Heart</topic><topic>Leukocyte Count</topic><topic>Lung</topic><topic>Lung Compliance</topic><topic>Organ Preservation - methods</topic><topic>Platelet Count</topic><topic>Pulmonary Artery - physiology</topic><topic>Rabbits</topic><topic>Radionuclide Imaging</topic><topic>Tissue Survival</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Genco, Christopher M.</creatorcontrib><creatorcontrib>Connolly, Raymond J.</creatorcontrib><creatorcontrib>Peterson, Myron B.</creatorcontrib><creatorcontrib>Bernstein, Eugene A.</creatorcontrib><creatorcontrib>Ramberg, Karen</creatorcontrib><creatorcontrib>Zhang, Xi</creatorcontrib><creatorcontrib>Cleveland, Richard J.</creatorcontrib><creatorcontrib>Diehl, James T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Annals of thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Genco, Christopher M.</au><au>Connolly, Raymond J.</au><au>Peterson, Myron B.</au><au>Bernstein, Eugene A.</au><au>Ramberg, Karen</au><au>Zhang, Xi</au><au>Cleveland, Richard J.</au><au>Diehl, James T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Granulocyte sequestration and early failure in the autoperfused heart-lung preparation</atitle><jtitle>The Annals of thoracic surgery</jtitle><addtitle>Ann Thorac Surg</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>53</volume><issue>2</issue><spage>217</spage><epage>226</epage><pages>217-226</pages><issn>0003-4975</issn><eissn>1552-6259</eissn><abstract>We investigated the role of pulmonary granulocyte sequestration in the development of early failure of the autoperfused working heart-lung preparation. A significant decline in the total circulating leukocyte count in 21 preparations at 60 minutes of perfusion (5.0 to 1.4 × 10
3/μL; 28% of baseline;
p < 0.001) was observed. Differential cell counts in 14 of these preparations revealed a predominant decrease in granulocyte count (8.7% of baseline) and a moderate decline in lymphocyte count (46% of baseline). In study I, indium 111-labeled autologous granulocytes were injected intravenously into 10 adult New Zealand While rabbits. In group I (n = 5), an autoperfused working heart-lung preparation was harvested and perfused for 60 minutes. In group II (controls, n = 5), the heart-lung block was harvested following 60 minutes of in situ perfusion. Organ blocks were imaged before and after saline flush. There was a significant decline in granulocyte counts at 60 minutes of perfusion in group I versus no change in group II (I, 2.3 ± 0.4 to 0.3 ± 0.1;
p < 0.01; II, 1.7 ± 0.2 to 2.3 ± 0.5; not significant; ×10
3/μL ± standard error of the mean). Postflush lung activity was significantly increased in group I versus group II (I, 3,751 ± 566; II, 1,867 ± 532;
p < 0.05; counts ± standard error of the mean). In study II, 15 autoperfused preparations were divided into two groups. Group I (n = 10) preparations were controls. Group II (n = 5) animals were depleted of leukocytes by pretreating with nitrogen mustard. Group I (controls) produced a bimodal survival distribution (Ia, 8.2 ± 1.0) Ib, 26.4 ± 2.0; hours ± standard error of the mean). Group II survival was significantly longer than that of group Ia and similar to that of group Ib (II, 25.3 ± 2.2;
p < 0.01 versus group Ia, not significant versus group Ib; hours ± standard error of the mean). Hemodynamic profiles for group II closely paralleled those of group Ib. In conclusion, pulmonary sequestration of granulocytes occurs early in the autoperfused working heart-lung preparation (within 60 minutes of autoperfusion), and preoperative leukocyte depletion prolongs survival of the autoperfused working heart-lung preparation by eliminating the subset group Ia (short survivors) seen in untreated preparations.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>1731660</pmid><doi>10.1016/0003-4975(92)91322-Z</doi><tpages>10</tpages></addata></record> |
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| ispartof | The Annals of thoracic surgery, 1992-02, Vol.53 (2), p.217-226 |
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| subjects | Animals Blood Gas Analysis Blood Pressure Cardiac Output Granulocytes - diagnostic imaging Granulocytes - physiology Heart Leukocyte Count Lung Lung Compliance Organ Preservation - methods Platelet Count Pulmonary Artery - physiology Rabbits Radionuclide Imaging Tissue Survival |
| title | Granulocyte sequestration and early failure in the autoperfused heart-lung preparation |
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