Carbohydrate-binding Modules Recognize Fine Substructures of Cellulose

Competition isotherms are used to identify the set of cellulose substructures to which cellulose binding modules (CBMs) from families 2a, 3, 4, 9, and 17 bind. The experiments are based on coupling a unique fluorescent tag to each CBM in a manner that does not alter the natural binding properties of...

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Veröffentlicht in:	The Journal of biological chemistry 2002-12, Vol.277 (52), p.50245-50254
Hauptverfasser:	McLean, Bradley W., Boraston, Alisdair B., Brouwer, Darren, Sanaie, Nooshafarin, Fyfe, Colin A., Warren, R. Antony J., Kilburn, Douglas G., Haynes, Charles A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Binding, Competitive Carbohydrates - chemistry Cellulase - metabolism Cellulose - chemistry Cellulose - metabolism Crystallization Kinetics Mass Spectrometry X-Ray Diffraction Xylan Endo-1,3-beta-Xylosidase Xylosidases - metabolism
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