Isolation and characterisation of a subpopulation of human chorionic cytotrophoblast using a monoclonal anti-trophoblast antibody (NDOG2) in flow cytometry
Human cytotrophoblast cells, isolated from term amniochorion by enzymic digestion and Percoll gradient centrifugation, were characterised by flow cytometry. A panel of 12 anti-trophoblast monoclonal antibodies was screened for labelling of these cells in flow cytometry and the results compared with...
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| Veröffentlicht in: | Journal of reproductive immunology 1992, Vol.21 (1), p.71-85 |
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| container_title | Journal of reproductive immunology |
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| creator | Caulfield, J.J. Sargent, I.L. Ferry, B.L. Starkey, P.M. Redman, C.W.G. |
| description | Human cytotrophoblast cells, isolated from term amniochorion by enzymic digestion and Percoll gradient centrifugation, were characterised by flow cytometry. A panel of 12 anti-trophoblast monoclonal antibodies was screened for labelling of these cells in flow cytometry and the results compared with immunoperoxidase labelling of cytospin preparations and tissue sections. All 12 antibodies were positive for trophoblast on tissue sections,
11
12
were positive on cytospins but only two (NDOG2 and GB25) gave consistent results in flow cytometry. Two-colour labelling with NDOG2 and W6/32, an antibody to HLA-A, -B, -C, demonstrated that 88% of the NDOG2-positive cells also express Class I major histocompatibility complex (MHC) antigens. The NDOG2-positive cytotrophoblast subpopulation was isolated by flow cytometry in sufficient purity (>95%) and yield (3.1 × 10
6) for use in functional studies in vitro. |
| doi_str_mv | 10.1016/0165-0378(92)90041-2 |
| format | Article |
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11
12
were positive on cytospins but only two (NDOG2 and GB25) gave consistent results in flow cytometry. Two-colour labelling with NDOG2 and W6/32, an antibody to HLA-A, -B, -C, demonstrated that 88% of the NDOG2-positive cells also express Class I major histocompatibility complex (MHC) antigens. The NDOG2-positive cytotrophoblast subpopulation was isolated by flow cytometry in sufficient purity (>95%) and yield (3.1 × 10
6) for use in functional studies in vitro.</description><identifier>ISSN: 0165-0378</identifier><identifier>EISSN: 1872-7603</identifier><identifier>DOI: 10.1016/0165-0378(92)90041-2</identifier><identifier>PMID: 1734079</identifier><identifier>CODEN: JRIMDR</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Antibodies, Monoclonal - immunology ; Antigens, Surface - analysis ; Biological and medical sciences ; Cell Separation - methods ; Chorion - cytology ; Chorion - immunology ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; human cytotrophoblast ; Humans ; monoclonal antibody ; Mother. Fetoplacental unit. Mammary gland. Milk ; Pregnancy ; Pregnancy. Parturition. Lactation ; Trophoblasts - cytology ; Trophoblasts - immunology ; Vertebrates: reproduction</subject><ispartof>Journal of reproductive immunology, 1992, Vol.21 (1), p.71-85</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-d3ec90619ebc875bae233076269ebf1125539a7b057c937a80ae2adf2e02297b3</citedby><cites>FETCH-LOGICAL-c417t-d3ec90619ebc875bae233076269ebf1125539a7b057c937a80ae2adf2e02297b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-0378(92)90041-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5564434$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1734079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caulfield, J.J.</creatorcontrib><creatorcontrib>Sargent, I.L.</creatorcontrib><creatorcontrib>Ferry, B.L.</creatorcontrib><creatorcontrib>Starkey, P.M.</creatorcontrib><creatorcontrib>Redman, C.W.G.</creatorcontrib><title>Isolation and characterisation of a subpopulation of human chorionic cytotrophoblast using a monoclonal anti-trophoblast antibody (NDOG2) in flow cytometry</title><title>Journal of reproductive immunology</title><addtitle>J Reprod Immunol</addtitle><description>Human cytotrophoblast cells, isolated from term amniochorion by enzymic digestion and Percoll gradient centrifugation, were characterised by flow cytometry. A panel of 12 anti-trophoblast monoclonal antibodies was screened for labelling of these cells in flow cytometry and the results compared with immunoperoxidase labelling of cytospin preparations and tissue sections. All 12 antibodies were positive for trophoblast on tissue sections,
11
12
were positive on cytospins but only two (NDOG2 and GB25) gave consistent results in flow cytometry. Two-colour labelling with NDOG2 and W6/32, an antibody to HLA-A, -B, -C, demonstrated that 88% of the NDOG2-positive cells also express Class I major histocompatibility complex (MHC) antigens. The NDOG2-positive cytotrophoblast subpopulation was isolated by flow cytometry in sufficient purity (>95%) and yield (3.1 × 10
6) for use in functional studies in vitro.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Separation - methods</subject><subject>Chorion - cytology</subject><subject>Chorion - immunology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human cytotrophoblast</subject><subject>Humans</subject><subject>monoclonal antibody</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>Pregnancy</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Trophoblasts - cytology</subject><subject>Trophoblasts - immunology</subject><subject>Vertebrates: reproduction</subject><issn>0165-0378</issn><issn>1872-7603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKkPhDUDyAqF2EfBPEsebSqhAqVTRDaytG8dhjBw72A5onqUvW08zGljBwrJ07neOru5B6CUlbymh7bvymopw0Z1Jdi4JqWnFHqEN7QSrREv4Y7Q5Ik_Rs5R-EEIFkfQEnVDBayLkBt1dp-Ag2-Ax-AHrLUTQ2USbVjGMGHBa-jnMiztK22UCX-AQi2A11rsccgzzNvQOUsZLsv57MU7BB-2CB1fSs63-ZvZCH4YdPvvy4faKnWPr8ejC74ewyeS4e46ejOCSeXH4T9G3Tx-_Xn6ubm6vri_f31S6piJXAzdakpZK0-tOND0YxjkRLWuLMlLKmoZLED1phJZcQEcKAcPIDGFMip6fojdr7hzDz8WkrCabtHEOvAlLUoKJrqOs_S9IyxKNkE0B6xXUMaQUzajmaCeIO0WJ2pen9s2ofTNKMvVQnmLF9uqQv_STGf6Y1rbK_PVhDkmDGyN4bdMRa5q2rnldsIsVM-Vov6yJKmlrvDaDjUZnNQT77z3uAVwnuHQ</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Caulfield, J.J.</creator><creator>Sargent, I.L.</creator><creator>Ferry, B.L.</creator><creator>Starkey, P.M.</creator><creator>Redman, C.W.G.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Isolation and characterisation of a subpopulation of human chorionic cytotrophoblast using a monoclonal anti-trophoblast antibody (NDOG2) in flow cytometry</title><author>Caulfield, J.J. ; Sargent, I.L. ; Ferry, B.L. ; Starkey, P.M. ; Redman, C.W.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d3ec90619ebc875bae233076269ebf1125539a7b057c937a80ae2adf2e02297b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Surface - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Separation - methods</topic><topic>Chorion - cytology</topic><topic>Chorion - immunology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human cytotrophoblast</topic><topic>Humans</topic><topic>monoclonal antibody</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>Pregnancy</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Trophoblasts - cytology</topic><topic>Trophoblasts - immunology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caulfield, J.J.</creatorcontrib><creatorcontrib>Sargent, I.L.</creatorcontrib><creatorcontrib>Ferry, B.L.</creatorcontrib><creatorcontrib>Starkey, P.M.</creatorcontrib><creatorcontrib>Redman, C.W.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of reproductive immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caulfield, J.J.</au><au>Sargent, I.L.</au><au>Ferry, B.L.</au><au>Starkey, P.M.</au><au>Redman, C.W.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterisation of a subpopulation of human chorionic cytotrophoblast using a monoclonal anti-trophoblast antibody (NDOG2) in flow cytometry</atitle><jtitle>Journal of reproductive immunology</jtitle><addtitle>J Reprod Immunol</addtitle><date>1992</date><risdate>1992</risdate><volume>21</volume><issue>1</issue><spage>71</spage><epage>85</epage><pages>71-85</pages><issn>0165-0378</issn><eissn>1872-7603</eissn><coden>JRIMDR</coden><abstract>Human cytotrophoblast cells, isolated from term amniochorion by enzymic digestion and Percoll gradient centrifugation, were characterised by flow cytometry. A panel of 12 anti-trophoblast monoclonal antibodies was screened for labelling of these cells in flow cytometry and the results compared with immunoperoxidase labelling of cytospin preparations and tissue sections. All 12 antibodies were positive for trophoblast on tissue sections,
11
12
were positive on cytospins but only two (NDOG2 and GB25) gave consistent results in flow cytometry. Two-colour labelling with NDOG2 and W6/32, an antibody to HLA-A, -B, -C, demonstrated that 88% of the NDOG2-positive cells also express Class I major histocompatibility complex (MHC) antigens. The NDOG2-positive cytotrophoblast subpopulation was isolated by flow cytometry in sufficient purity (>95%) and yield (3.1 × 10
6) for use in functional studies in vitro.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>1734079</pmid><doi>10.1016/0165-0378(92)90041-2</doi><tpages>15</tpages></addata></record> |
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| ispartof | Journal of reproductive immunology, 1992, Vol.21 (1), p.71-85 |
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| language | eng |
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| subjects | Antibodies, Monoclonal - immunology Antigens, Surface - analysis Biological and medical sciences Cell Separation - methods Chorion - cytology Chorion - immunology Female Flow Cytometry Fundamental and applied biological sciences. Psychology human cytotrophoblast Humans monoclonal antibody Mother. Fetoplacental unit. Mammary gland. Milk Pregnancy Pregnancy. Parturition. Lactation Trophoblasts - cytology Trophoblasts - immunology Vertebrates: reproduction |
| title | Isolation and characterisation of a subpopulation of human chorionic cytotrophoblast using a monoclonal anti-trophoblast antibody (NDOG2) in flow cytometry |
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