Chemical cross-linking of the cytosolic and nuclear forms of the Ah receptor in hepatoma cell line 1c1c7

Both cytosolic and high salt nuclear extracts were isolated from Hepa 1c1c7 cells incubated with 2-azido-3[ 1251]iodo-7, 8-dibromo-dibenzo- p-dioxin ([ 125I]N 3Br 2D pD). The [ 125I]N 3Br 2D pD-labeled cytosolic fraction was subjected to chemical cross-linking with dimethyl pimelimidate and analyzed...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-01, Vol.182 (1), p.55-62
1. Verfasser:	Perdew, Gary H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Carcinoma, Hepatocellular Cell Fractionation Cell Line Cell Nucleus - metabolism Cell receptors Cell structures and functions Centrifugation, Density Gradient Cross-Linking Reagents Cytosol - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Liver Neoplasms Macromolecular Substances Miscellaneous Molecular and cellular biology Molecular Weight Polychlorinated Dibenzodioxins - metabolism Polyenes - pharmacology Receptors, Aryl Hydrocarbon Receptors, Drug - isolation & purification Receptors, Drug - metabolism
Online-Zugang:	Volltext
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