CDK5 Regulates Cell Adhesion and Migration in Corneal Epithelial Cells
CDK5 and its activator, p35, are expressed in mouse corneal epithelium and can be coimmunoprecipited from corneal epithelial cell lysates. Immunostaining shows CDK5 and p35 in all layers of the corneal epithelium, especially along the basal side of the basal cells. Stable transfection of corneal epi...
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| Veröffentlicht in: | Molecular cancer research 2002-11, Vol.1 (1), p.12-24 |
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| creator | CHUN GAO NEGASH, Sewite HONG TAO GUO LEDEE, Dolena WANG, Hwai-Shi ZELENKA, Peggy |
| description | CDK5 and its activator, p35, are expressed in mouse corneal epithelium and can be coimmunoprecipited from corneal epithelial
cell lysates. Immunostaining shows CDK5 and p35 in all layers of the corneal epithelium, especially along the basal side of
the basal cells. Stable transfection of corneal epithelial cells with CDK5, which increases CDK5 kinase activity by approximately
33%, also increases the number of cells adhering to fibronectin and the strength of adhesion. CDK5 kinase activity seems to
be required for this effect, because the kinase inactive mutation, CDK5-T33, either reduces adhesion or has no significant
effect, depending on the level of expression. Using an in vitro scrape wound in confluent cultures of stably transfected cells to examine the effect of CDK5 on cell migration, we show that
reoccupation of the wound area is significantly decreased by CDK5 and increased by CDK5-T33. These findings indicate that
CDK5 may be an important regulator of adhesion and migration of corneal epithelial cells. |
| format | Article |
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cell lysates. Immunostaining shows CDK5 and p35 in all layers of the corneal epithelium, especially along the basal side of
the basal cells. Stable transfection of corneal epithelial cells with CDK5, which increases CDK5 kinase activity by approximately
33%, also increases the number of cells adhering to fibronectin and the strength of adhesion. CDK5 kinase activity seems to
be required for this effect, because the kinase inactive mutation, CDK5-T33, either reduces adhesion or has no significant
effect, depending on the level of expression. Using an in vitro scrape wound in confluent cultures of stably transfected cells to examine the effect of CDK5 on cell migration, we show that
reoccupation of the wound area is significantly decreased by CDK5 and increased by CDK5-T33. These findings indicate that
CDK5 may be an important regulator of adhesion and migration of corneal epithelial cells.</description><identifier>ISSN: 1541-7786</identifier><identifier>EISSN: 1557-3125</identifier><identifier>PMID: 12496365</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>adhesion ; Animals ; Biological and medical sciences ; CDK5 ; Cell Adhesion ; Cell interactions, adhesion ; Cell Line ; Cell Movement ; Cornea - cytology ; Cyclin-Dependent Kinase 5 ; Cyclin-Dependent Kinases - genetics ; Cyclin-Dependent Kinases - physiology ; cytoskeleton ; Cytoskeleton - metabolism ; Cytosol - metabolism ; Epithelial Cells - cytology ; Fibronectins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Green Fluorescent Proteins ; Intracellular Membranes - metabolism ; Luminescent Proteins - metabolism ; Mice ; Mice, Mutant Strains ; migration ; Molecular and cellular biology ; Mutation ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; p35 ; Recombinant Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Transfection ; Vinculin - metabolism</subject><ispartof>Molecular cancer research, 2002-11, Vol.1 (1), p.12-24</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14435790$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12496365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHUN GAO</creatorcontrib><creatorcontrib>NEGASH, Sewite</creatorcontrib><creatorcontrib>HONG TAO GUO</creatorcontrib><creatorcontrib>LEDEE, Dolena</creatorcontrib><creatorcontrib>WANG, Hwai-Shi</creatorcontrib><creatorcontrib>ZELENKA, Peggy</creatorcontrib><title>CDK5 Regulates Cell Adhesion and Migration in Corneal Epithelial Cells</title><title>Molecular cancer research</title><addtitle>Mol Cancer Res</addtitle><description>CDK5 and its activator, p35, are expressed in mouse corneal epithelium and can be coimmunoprecipited from corneal epithelial
cell lysates. Immunostaining shows CDK5 and p35 in all layers of the corneal epithelium, especially along the basal side of
the basal cells. Stable transfection of corneal epithelial cells with CDK5, which increases CDK5 kinase activity by approximately
33%, also increases the number of cells adhering to fibronectin and the strength of adhesion. CDK5 kinase activity seems to
be required for this effect, because the kinase inactive mutation, CDK5-T33, either reduces adhesion or has no significant
effect, depending on the level of expression. Using an in vitro scrape wound in confluent cultures of stably transfected cells to examine the effect of CDK5 on cell migration, we show that
reoccupation of the wound area is significantly decreased by CDK5 and increased by CDK5-T33. These findings indicate that
CDK5 may be an important regulator of adhesion and migration of corneal epithelial cells.</description><subject>adhesion</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CDK5</subject><subject>Cell Adhesion</subject><subject>Cell interactions, adhesion</subject><subject>Cell Line</subject><subject>Cell Movement</subject><subject>Cornea - cytology</subject><subject>Cyclin-Dependent Kinase 5</subject><subject>Cyclin-Dependent Kinases - genetics</subject><subject>Cyclin-Dependent Kinases - physiology</subject><subject>cytoskeleton</subject><subject>Cytoskeleton - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Green Fluorescent Proteins</subject><subject>Intracellular Membranes - metabolism</subject><subject>Luminescent Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>migration</subject><subject>Molecular and cellular biology</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>p35</subject><subject>Recombinant Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Transfection</subject><subject>Vinculin - metabolism</subject><issn>1541-7786</issn><issn>1557-3125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFz19LwzAQAPAiipvTryB50bdC_jRJ8zjqNsWJIPpcrul1jXTtTFrEb2-Lk3EPdwe_O-7OojmTUseCcXk-1QmLtU7VLLoK4ZNSTplWl9GM8cQooeQ8WmcPz5K84W5ooMdAMmwasixrDK5rCbQleXE7D_3UuZZknW8RGrI6uL7Gxo3lNBGuo4sKmoA3x7yIPtar9-wx3r5unrLlNq650n0sqwSt4pgwLApWppJBhYKBEpyxyoC0nEJZqpTKigos0KBR2hSFhkpSw8Qiuv_be_Dd14Chz_cu2PECaLEbQq65TrWmZoS3RzgUeyzzg3d78D_5_-cjuDsCCBaaykNrXTi5JBFSG3pytdvV385jbkeJ3mNA8LbO2RRc_AKR9G7b</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>CHUN GAO</creator><creator>NEGASH, Sewite</creator><creator>HONG TAO GUO</creator><creator>LEDEE, Dolena</creator><creator>WANG, Hwai-Shi</creator><creator>ZELENKA, Peggy</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>CDK5 Regulates Cell Adhesion and Migration in Corneal Epithelial Cells</title><author>CHUN GAO ; NEGASH, Sewite ; HONG TAO GUO ; LEDEE, Dolena ; WANG, Hwai-Shi ; ZELENKA, Peggy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-5f4ec62e41ebb1d851afe31a63211f9a5c20add6805f03ebe9e9679bb7af50913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>adhesion</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CDK5</topic><topic>Cell Adhesion</topic><topic>Cell interactions, adhesion</topic><topic>Cell Line</topic><topic>Cell Movement</topic><topic>Cornea - cytology</topic><topic>Cyclin-Dependent Kinase 5</topic><topic>Cyclin-Dependent Kinases - genetics</topic><topic>Cyclin-Dependent Kinases - physiology</topic><topic>cytoskeleton</topic><topic>Cytoskeleton - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Green Fluorescent Proteins</topic><topic>Intracellular Membranes - metabolism</topic><topic>Luminescent Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>migration</topic><topic>Molecular and cellular biology</topic><topic>Mutation</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>p35</topic><topic>Recombinant Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Transfection</topic><topic>Vinculin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHUN GAO</creatorcontrib><creatorcontrib>NEGASH, Sewite</creatorcontrib><creatorcontrib>HONG TAO GUO</creatorcontrib><creatorcontrib>LEDEE, Dolena</creatorcontrib><creatorcontrib>WANG, Hwai-Shi</creatorcontrib><creatorcontrib>ZELENKA, Peggy</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHUN GAO</au><au>NEGASH, Sewite</au><au>HONG TAO GUO</au><au>LEDEE, Dolena</au><au>WANG, Hwai-Shi</au><au>ZELENKA, Peggy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CDK5 Regulates Cell Adhesion and Migration in Corneal Epithelial Cells</atitle><jtitle>Molecular cancer research</jtitle><addtitle>Mol Cancer Res</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>1</volume><issue>1</issue><spage>12</spage><epage>24</epage><pages>12-24</pages><issn>1541-7786</issn><eissn>1557-3125</eissn><abstract>CDK5 and its activator, p35, are expressed in mouse corneal epithelium and can be coimmunoprecipited from corneal epithelial
cell lysates. Immunostaining shows CDK5 and p35 in all layers of the corneal epithelium, especially along the basal side of
the basal cells. Stable transfection of corneal epithelial cells with CDK5, which increases CDK5 kinase activity by approximately
33%, also increases the number of cells adhering to fibronectin and the strength of adhesion. CDK5 kinase activity seems to
be required for this effect, because the kinase inactive mutation, CDK5-T33, either reduces adhesion or has no significant
effect, depending on the level of expression. Using an in vitro scrape wound in confluent cultures of stably transfected cells to examine the effect of CDK5 on cell migration, we show that
reoccupation of the wound area is significantly decreased by CDK5 and increased by CDK5-T33. These findings indicate that
CDK5 may be an important regulator of adhesion and migration of corneal epithelial cells.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>12496365</pmid><tpages>13</tpages></addata></record> |
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| subjects | adhesion Animals Biological and medical sciences CDK5 Cell Adhesion Cell interactions, adhesion Cell Line Cell Movement Cornea - cytology Cyclin-Dependent Kinase 5 Cyclin-Dependent Kinases - genetics Cyclin-Dependent Kinases - physiology cytoskeleton Cytoskeleton - metabolism Cytosol - metabolism Epithelial Cells - cytology Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation Green Fluorescent Proteins Intracellular Membranes - metabolism Luminescent Proteins - metabolism Mice Mice, Mutant Strains migration Molecular and cellular biology Mutation Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism p35 Recombinant Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Transfection Vinculin - metabolism |
| title | CDK5 Regulates Cell Adhesion and Migration in Corneal Epithelial Cells |
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