Effect of an orally applied herbal immunomodulator on cytokine induction and antibody response in normal and immunosuppressed mice
The influence of the oral administration of a herbal immunomodulator, consisting of an aqueous-ethanolic extract of the mixed herbal drugs Thujae summitates, Baptisiae tinctoriae radix, Echinaceae purpureae radix and Echinaceae pallidae radix, on cytokine induction and antibody response against shee...
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| Veröffentlicht in: | Phytomedicine (Stuttgart) 2002-10, Vol.9 (7), p.606-613 |
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| creator | Bodinet, C. Lindequist, U. Teuscher, E. Freudenstein, J. |
| description | The influence of the oral administration of a herbal immunomodulator, consisting of an aqueous-ethanolic extract of the mixed herbal drugs Thujae summitates, Baptisiae tinctoriae radix, Echinaceae purpureae radix and Echinaceae pallidae radix, on cytokine induction and antibody response against sheep red blood cells was investigated in mice. The treatment of the animals with the extract caused no enhancement of the cytokine titers in the serum. Spleen cells isolated from the treated mice, however, produced higher amounts of IL-2, IFNγ and GM-CSF
ex vivo in comparison to spleen cells isolated from control animals, especially after additional stimulation by lipopolysaccharides or concanavalin A. The application of the extract also triggered the production of IL-1 and TNFα by peritoneal macrophages
ex vivo. The influence of the herbal extract on the antibody response was examined by the plaque forming cell assay. The administration of the extract caused a significant enhancement of the antibody response against sheep red blood cells, inducing an increase in the numbers of splenic plaque forming cells and the titers of specific antibodies in the sera of the treated animals. In mice, immunosuppressed by old age or additional treatment with hydrocortisone, the therapy with the extract resulted in a normalization of the antibody response against sheep red blood cells. |
| doi_str_mv | 10.1078/094471102321616418 |
| format | Article |
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ex vivo in comparison to spleen cells isolated from control animals, especially after additional stimulation by lipopolysaccharides or concanavalin A. The application of the extract also triggered the production of IL-1 and TNFα by peritoneal macrophages
ex vivo. The influence of the herbal extract on the antibody response was examined by the plaque forming cell assay. The administration of the extract caused a significant enhancement of the antibody response against sheep red blood cells, inducing an increase in the numbers of splenic plaque forming cells and the titers of specific antibodies in the sera of the treated animals. In mice, immunosuppressed by old age or additional treatment with hydrocortisone, the therapy with the extract resulted in a normalization of the antibody response against sheep red blood cells.</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1078/094471102321616418</identifier><identifier>PMID: 12487324</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adjuvants, Immunologic - blood ; Adjuvants, Immunologic - pharmacology ; Administration, Oral ; Animals ; Antibody Formation - drug effects ; Antibody Formation - immunology ; Baptisia spp ; cytokine induction ; Cytokines - biosynthesis ; Cytokines - blood ; Cytokines - drug effects ; Echinacea - chemistry ; Echinacea spp ; Erythrocytes - immunology ; Fabaceae - chemistry ; Herbal immunomodulator ; Interferon-gamma - biosynthesis ; Interferon-gamma - drug effects ; Interleukin-1 - biosynthesis ; Interleukin-2 - biosynthesis ; Macrophages - drug effects ; Male ; Mice ; Mice, Inbred C3H ; Mice, Inbred Strains ; peroral application ; PFC response in mice ; Plant Extracts - pharmacology ; Sheep ; Spleen - cytology ; Spleen - drug effects ; Thuja - chemistry ; Thuja spp ; Tumor Necrosis Factor-alpha - biosynthesis</subject><ispartof>Phytomedicine (Stuttgart), 2002-10, Vol.9 (7), p.606-613</ispartof><rights>2002 Urban & Fischer Verlag</rights><rights>COPYRIGHT 2002 Urban & Fischer Verlag</rights><rights>Copyright Urban & Fischer Verlag Oct 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-b6697ac8825a45f137db7fb6cde54c9214cbebea4583ec8edf80137a138971c03</citedby><cites>FETCH-LOGICAL-c481t-b6697ac8825a45f137db7fb6cde54c9214cbebea4583ec8edf80137a138971c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944711304701634$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12487324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bodinet, C.</creatorcontrib><creatorcontrib>Lindequist, U.</creatorcontrib><creatorcontrib>Teuscher, E.</creatorcontrib><creatorcontrib>Freudenstein, J.</creatorcontrib><title>Effect of an orally applied herbal immunomodulator on cytokine induction and antibody response in normal and immunosuppressed mice</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>The influence of the oral administration of a herbal immunomodulator, consisting of an aqueous-ethanolic extract of the mixed herbal drugs Thujae summitates, Baptisiae tinctoriae radix, Echinaceae purpureae radix and Echinaceae pallidae radix, on cytokine induction and antibody response against sheep red blood cells was investigated in mice. The treatment of the animals with the extract caused no enhancement of the cytokine titers in the serum. Spleen cells isolated from the treated mice, however, produced higher amounts of IL-2, IFNγ and GM-CSF
ex vivo in comparison to spleen cells isolated from control animals, especially after additional stimulation by lipopolysaccharides or concanavalin A. The application of the extract also triggered the production of IL-1 and TNFα by peritoneal macrophages
ex vivo. The influence of the herbal extract on the antibody response was examined by the plaque forming cell assay. The administration of the extract caused a significant enhancement of the antibody response against sheep red blood cells, inducing an increase in the numbers of splenic plaque forming cells and the titers of specific antibodies in the sera of the treated animals. In mice, immunosuppressed by old age or additional treatment with hydrocortisone, the therapy with the extract resulted in a normalization of the antibody response against sheep red blood cells.</description><subject>Adjuvants, Immunologic - blood</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Administration, Oral</subject><subject>Animals</subject><subject>Antibody Formation - drug effects</subject><subject>Antibody Formation - immunology</subject><subject>Baptisia spp</subject><subject>cytokine induction</subject><subject>Cytokines - biosynthesis</subject><subject>Cytokines - blood</subject><subject>Cytokines - drug effects</subject><subject>Echinacea - chemistry</subject><subject>Echinacea spp</subject><subject>Erythrocytes - immunology</subject><subject>Fabaceae - chemistry</subject><subject>Herbal immunomodulator</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - drug effects</subject><subject>Interleukin-1 - biosynthesis</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Macrophages - drug effects</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, Inbred Strains</subject><subject>peroral application</subject><subject>PFC response in mice</subject><subject>Plant Extracts - pharmacology</subject><subject>Sheep</subject><subject>Spleen - cytology</subject><subject>Spleen - drug effects</subject><subject>Thuja - chemistry</subject><subject>Thuja spp</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kk2L1TAUhoMoznX0D7iQ4MKV1Xy1ScHNMIwfMOBGwV1Ik5MxY5vUpB24W3-5KffCiF4khIRznvOefLwIPafkDSVSvSW9EJJSwjijHe0EVQ_Qru5UQ_r220O024CmEvwMPSnllhAqekkeozPKhJKciR36deU92AUnj03EKZtx3GMzz2MAh79DHsyIwzStMU3JraNZUsYpYrtf0o8QAYfoVruEGjLR1bmEIbk9zlDmFMuWxzHlqaps-YNSWee5AqV2mIKFp-iRN2OBZ8f1HH19f_Xl8mNz_fnDp8uL68YKRZdm6LpeGqsUa41oPeXSDdIPnXXQCtszKuwAA9Sc4mAVOK9IhQzlqpfUEn6OXh1055x-rlAWPYViYRxNhLQWLZlUnDFWwZd_gbdpzbGeTTPStoxL0t9DN2YEHaJPSzZ2U9QXfcc62fGt5esT0A1EqA-dIvhQw3_izQm8Dgf1oU7x7MDbnErJ4PWcw2TyXlOiN4vofy1Si14cb7cOE7j7kqMnKvDuAED9jLsAWRcbIFpwIVeraJfC__R_AzFRyd4</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Bodinet, C.</creator><creator>Lindequist, U.</creator><creator>Teuscher, E.</creator><creator>Freudenstein, J.</creator><general>Elsevier GmbH</general><general>Urban & Fischer Verlag</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Effect of an orally applied herbal immunomodulator on cytokine induction and antibody response in normal and immunosuppressed mice</title><author>Bodinet, C. ; Lindequist, U. ; Teuscher, E. ; Freudenstein, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-b6697ac8825a45f137db7fb6cde54c9214cbebea4583ec8edf80137a138971c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adjuvants, Immunologic - blood</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Administration, Oral</topic><topic>Animals</topic><topic>Antibody Formation - drug effects</topic><topic>Antibody Formation - immunology</topic><topic>Baptisia spp</topic><topic>cytokine induction</topic><topic>Cytokines - biosynthesis</topic><topic>Cytokines - blood</topic><topic>Cytokines - drug effects</topic><topic>Echinacea - chemistry</topic><topic>Echinacea spp</topic><topic>Erythrocytes - immunology</topic><topic>Fabaceae - chemistry</topic><topic>Herbal immunomodulator</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - drug effects</topic><topic>Interleukin-1 - biosynthesis</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Macrophages - drug effects</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mice, Inbred Strains</topic><topic>peroral application</topic><topic>PFC response in mice</topic><topic>Plant Extracts - pharmacology</topic><topic>Sheep</topic><topic>Spleen - 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Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodinet, C.</au><au>Lindequist, U.</au><au>Teuscher, E.</au><au>Freudenstein, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of an orally applied herbal immunomodulator on cytokine induction and antibody response in normal and immunosuppressed mice</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>9</volume><issue>7</issue><spage>606</spage><epage>613</epage><pages>606-613</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>The influence of the oral administration of a herbal immunomodulator, consisting of an aqueous-ethanolic extract of the mixed herbal drugs Thujae summitates, Baptisiae tinctoriae radix, Echinaceae purpureae radix and Echinaceae pallidae radix, on cytokine induction and antibody response against sheep red blood cells was investigated in mice. The treatment of the animals with the extract caused no enhancement of the cytokine titers in the serum. Spleen cells isolated from the treated mice, however, produced higher amounts of IL-2, IFNγ and GM-CSF
ex vivo in comparison to spleen cells isolated from control animals, especially after additional stimulation by lipopolysaccharides or concanavalin A. The application of the extract also triggered the production of IL-1 and TNFα by peritoneal macrophages
ex vivo. The influence of the herbal extract on the antibody response was examined by the plaque forming cell assay. The administration of the extract caused a significant enhancement of the antibody response against sheep red blood cells, inducing an increase in the numbers of splenic plaque forming cells and the titers of specific antibodies in the sera of the treated animals. In mice, immunosuppressed by old age or additional treatment with hydrocortisone, the therapy with the extract resulted in a normalization of the antibody response against sheep red blood cells.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>12487324</pmid><doi>10.1078/094471102321616418</doi><tpages>8</tpages></addata></record> |
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| subjects | Adjuvants, Immunologic - blood Adjuvants, Immunologic - pharmacology Administration, Oral Animals Antibody Formation - drug effects Antibody Formation - immunology Baptisia spp cytokine induction Cytokines - biosynthesis Cytokines - blood Cytokines - drug effects Echinacea - chemistry Echinacea spp Erythrocytes - immunology Fabaceae - chemistry Herbal immunomodulator Interferon-gamma - biosynthesis Interferon-gamma - drug effects Interleukin-1 - biosynthesis Interleukin-2 - biosynthesis Macrophages - drug effects Male Mice Mice, Inbred C3H Mice, Inbred Strains peroral application PFC response in mice Plant Extracts - pharmacology Sheep Spleen - cytology Spleen - drug effects Thuja - chemistry Thuja spp Tumor Necrosis Factor-alpha - biosynthesis |
| title | Effect of an orally applied herbal immunomodulator on cytokine induction and antibody response in normal and immunosuppressed mice |
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