Detection of Individual p53-Autoantibodies by Using Quenched Peptide-Based Molecular Probes
Single‐molecule spectroscopy for cancer diagnostics: Short dye‐labeled peptide epitopes derived from human p53 show strong fluorescence quenching owing to efficient electron transfer from a tryptophan residue to the fluorophore (1, see picture). The specific recognition of the peptide epitope by the...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2002-12, Vol.41 (24), p.4769-4773 |
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| container_end_page | 4773 |
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| container_issue | 24 |
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| container_title | Angewandte Chemie International Edition |
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| creator | Neuweiler, Hannes Schulz, Andreas Vaiana, Andrea C. Smith, Jeremy C. Kaul, Sepp Wolfrum, Jürgen Sauer, Markus |
| description | Single‐molecule spectroscopy for cancer diagnostics: Short dye‐labeled peptide epitopes derived from human p53 show strong fluorescence quenching owing to efficient electron transfer from a tryptophan residue to the fluorophore (1, see picture). The specific recognition of the peptide epitope by the antibody induces a change in the conformation which is accompanied by a strong increase in fluorescence intensity. The fluorescing antibody–peptide complex can be detected at the single‐molecule level even in slightly diluted serum samples by using confocal fluorescence microscopy. |
| doi_str_mv | 10.1002/anie.200290044 |
| format | Article |
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The specific recognition of the peptide epitope by the antibody induces a change in the conformation which is accompanied by a strong increase in fluorescence intensity. 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The specific recognition of the peptide epitope by the antibody induces a change in the conformation which is accompanied by a strong increase in fluorescence intensity. The fluorescing antibody–peptide complex can be detected at the single‐molecule level even in slightly diluted serum samples by using confocal fluorescence microscopy.</description><subject>Amino Acid Sequence</subject><subject>antibodies</subject><subject>Autoantibodies - analysis</subject><subject>Autoantibodies - immunology</subject><subject>Coloring Agents</subject><subject>Epitopes - chemistry</subject><subject>fluorescent probes</subject><subject>hydrophobic effect</subject><subject>immunoassays</subject><subject>Molecular Probes - analysis</subject><subject>Molecular Probes - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Peptides - chemistry</subject><subject>Protein Binding</subject><subject>Sensitivity and Specificity</subject><subject>single-molecule studies</subject><subject>Spectrometry, Fluorescence</subject><subject>Staining and Labeling</subject><subject>Tumor Suppressor Protein p53 - immunology</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0E4ntlRJnYUuzYjpOx0FIqQSmiFQOD5dhXMKRxiROg_x5XrYCN6e6k5310ehE6IbhDME7OVWWhk4Qtx5ixLbRPeEJiKgTdDjujNBYZJ3vowPvXQGUZTnfRHklYRihn--ipBw3oxroqcrNoWBn7YU2rymjBadxtG6eqxhbOWPBRsYym3lbP0X0LlX4BE41h0VgD8YXy4bp1Jei2VHU0rl0B_gjtzFTp4XgzD9H0qj-5vI5v7gbDy-5NrBnjLGagAXKlUpIIoTIQhTGZhhwXuQk_znIuGOWCFwQMGI0TYghJMyJYDgwA6CE6W3sXtXtvwTdybr2GslQVuNZLEbwpZnkAO2tQ1877GmZyUdu5qpeSYLmqU67qlD91hsDpxtwWczC_-Ka_AORr4NOWsPxHJ7ujYf-vPF5nrW_g6yer6jeZCiq4fBwN5GNvIEZk8iAx_QagLZD-</recordid><startdate>20021216</startdate><enddate>20021216</enddate><creator>Neuweiler, Hannes</creator><creator>Schulz, Andreas</creator><creator>Vaiana, Andrea C.</creator><creator>Smith, Jeremy C.</creator><creator>Kaul, Sepp</creator><creator>Wolfrum, Jürgen</creator><creator>Sauer, Markus</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021216</creationdate><title>Detection of Individual p53-Autoantibodies by Using Quenched Peptide-Based Molecular Probes</title><author>Neuweiler, Hannes ; 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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Amino Acid Sequence antibodies Autoantibodies - analysis Autoantibodies - immunology Coloring Agents Epitopes - chemistry fluorescent probes hydrophobic effect immunoassays Molecular Probes - analysis Molecular Probes - chemistry Molecular Sequence Data Peptides - chemistry Protein Binding Sensitivity and Specificity single-molecule studies Spectrometry, Fluorescence Staining and Labeling Tumor Suppressor Protein p53 - immunology |
| title | Detection of Individual p53-Autoantibodies by Using Quenched Peptide-Based Molecular Probes |
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