E. coli Expression of TIMP-4 and Comparative Kinetic Studies with TIMP-1 and TIMP-2: Insights into the Interactions of TIMPs and Matrix Metalloproteinase 2 (Gelatinase A)

The inhibitory properties of TIMP-4 for matrix metalloproteinases (MMPs) were compared to those of TIMP-1 and TIMP-2. Full-length human TIMP-4 was expressed in E. coli, folded from inclusion bodies, and the active component was purified by MMP-1 affinity chromatography. Progress curve analysis of MM...

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Veröffentlicht in:	Biochemistry (Easton) 2002-12, Vol.41 (50), p.15025-15035
Hauptverfasser:	Troeberg, Linda, Tanaka, Mitsuo, Wait, Robin, Shi, Yeunian E, Brew, Keith, Nagase, Hideaki
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Macroglobulins - chemistry alpha-Macroglobulins - metabolism Amino Acid Sequence Animals Binding, Competitive CHO Cells Cricetinae Escherichia coli - genetics Humans Hydrogen-Ion Concentration Kinetics Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase Inhibitors Molecular Sequence Data Protein Folding Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - metabolism Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tissue Inhibitor of Metalloproteinase-1 - metabolism Tissue Inhibitor of Metalloproteinase-2 - metabolism Tissue Inhibitor of Metalloproteinase-4 Tissue Inhibitor of Metalloproteinases - biosynthesis Tissue Inhibitor of Metalloproteinases - chemistry Tissue Inhibitor of Metalloproteinases - genetics Tissue Inhibitor of Metalloproteinases - metabolism
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