In vitro effects of thyroid hormones on ectonucleotidase activities in synaptosomes from hippocampus of rats
1. Studies have shown that adenosine transport and adenosine A1 receptors in rat brain are subjected to regulation by thyroid hormone levels. Since the ectonucleotidase pathway is an important source of adenosine extracellular, in the present study the in vitro action of T3 and T4 hormones on ectonu...
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| Veröffentlicht in: | Cellular and molecular neurobiology 2002-06, Vol.22 (3), p.345-352 |
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| container_title | Cellular and molecular neurobiology |
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| creator | Matos, José Alfredo de Aguiar Bruno, Alessandra Nejar Oses, Jean Pierre Bonan, Carla Denise Battastini, Ana Maria Oliveira Barreto-Chaves, Maria Luiza M Sarkis, João José Freitas |
| description | 1. Studies have shown that adenosine transport and adenosine A1 receptors in rat brain are subjected to regulation by thyroid hormone levels. Since the ectonucleotidase pathway is an important source of adenosine extracellular, in the present study the in vitro action of T3 and T4 hormones on ectonucleotidase activities in hippocampal synaptosomes was evaluated. 2. T3 (Triiodo-l-thyronine) significantly inhibited, in an uncompetitive manner, the ATP and ADP hydrolysis promoted by ATP diphosphohydrolase activity in hippocampal synaptosomes of adult rats. 3. In contrast, T4 (Thyroxine) only inhibited ATP hydrolysis in an uncompetitive mechanism, at the concentrations tested (100-500 microM), but at the same time did not affect ADP hydrolysis. 4. In the present study, we also investigate the in vitro effect of T3 and T4 on 5'-nucleotidase activity. However, there are no changes in the activity of this enzyme in the presence of T3 and T4 in the hippocampal synaptosomes of rats. 5. These results suggest that thyroid hormones could be involved in the regulation of ectonucleotidase activities, such as ecto-ATP diphosphohydrolase and ecto-ATPase, possibly exerting a modulatory role in extracellular adenosine levels. |
| doi_str_mv | 10.1023/A:1020776119612 |
| format | Article |
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Studies have shown that adenosine transport and adenosine A1 receptors in rat brain are subjected to regulation by thyroid hormone levels. Since the ectonucleotidase pathway is an important source of adenosine extracellular, in the present study the in vitro action of T3 and T4 hormones on ectonucleotidase activities in hippocampal synaptosomes was evaluated. 2. T3 (Triiodo-l-thyronine) significantly inhibited, in an uncompetitive manner, the ATP and ADP hydrolysis promoted by ATP diphosphohydrolase activity in hippocampal synaptosomes of adult rats. 3. In contrast, T4 (Thyroxine) only inhibited ATP hydrolysis in an uncompetitive mechanism, at the concentrations tested (100-500 microM), but at the same time did not affect ADP hydrolysis. 4. In the present study, we also investigate the in vitro effect of T3 and T4 on 5'-nucleotidase activity. However, there are no changes in the activity of this enzyme in the presence of T3 and T4 in the hippocampal synaptosomes of rats. 5. 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Studies have shown that adenosine transport and adenosine A1 receptors in rat brain are subjected to regulation by thyroid hormone levels. Since the ectonucleotidase pathway is an important source of adenosine extracellular, in the present study the in vitro action of T3 and T4 hormones on ectonucleotidase activities in hippocampal synaptosomes was evaluated. 2. T3 (Triiodo-l-thyronine) significantly inhibited, in an uncompetitive manner, the ATP and ADP hydrolysis promoted by ATP diphosphohydrolase activity in hippocampal synaptosomes of adult rats. 3. In contrast, T4 (Thyroxine) only inhibited ATP hydrolysis in an uncompetitive mechanism, at the concentrations tested (100-500 microM), but at the same time did not affect ADP hydrolysis. 4. In the present study, we also investigate the in vitro effect of T3 and T4 on 5'-nucleotidase activity. However, there are no changes in the activity of this enzyme in the presence of T3 and T4 in the hippocampal synaptosomes of rats. 5. These results suggest that thyroid hormones could be involved in the regulation of ectonucleotidase activities, such as ecto-ATP diphosphohydrolase and ecto-ATPase, possibly exerting a modulatory role in extracellular adenosine levels.</description><subject>Adenosine - metabolism</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Monophosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Extracellular Space - drug effects</subject><subject>Extracellular Space - metabolism</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - enzymology</subject><subject>Hydrolysis - drug effects</subject><subject>Male</subject><subject>Presynaptic Terminals - drug effects</subject><subject>Presynaptic Terminals - enzymology</subject><subject>Pyrophosphatases - drug effects</subject><subject>Pyrophosphatases - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Synaptosomes - drug effects</subject><subject>Synaptosomes - metabolism</subject><subject>Thyroid Gland - metabolism</subject><subject>Thyroid Hormones - metabolism</subject><subject>Thyroid Hormones - pharmacology</subject><subject>Thyroxine - metabolism</subject><subject>Thyroxine - pharmacology</subject><subject>Triiodothyronine - metabolism</subject><subject>Triiodothyronine - pharmacology</subject><issn>0272-4340</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAQxT2AaCnMbMgTW8DfjtmqikKlSiwwR45jq0ZJHGwHqf89FpSZ6enu_e5J7wC4wegeI0If1o9FkJQCYyUwOQNLRCSpGGVoAS5T-kAIKYT4BVhgwoSqJV-CfjfCL59jgNY5a3KCwcF8OMbgO3gIcQijLbsRFi-Ms-ltyL7TyUJtsi-Xvth-hOk46imHFIYyuxgGePDTFIwepvknM-qcrsC5032y1yddgfft09vmpdq_Pu82631lKOG5UoZp7BSjXU0cxtQog5gkui39GMNEOCopcaLTLcdUS9Ey1KrSxxKLjeroCtz95k4xfM425Wbwydi-16MNc2okkZwozv8FcS2oqKUo4O0JnNvBds0U_aDjsfl7JP0GXll0mA</recordid><startdate>200206</startdate><enddate>200206</enddate><creator>Matos, José Alfredo de Aguiar</creator><creator>Bruno, Alessandra Nejar</creator><creator>Oses, Jean Pierre</creator><creator>Bonan, Carla Denise</creator><creator>Battastini, Ana Maria Oliveira</creator><creator>Barreto-Chaves, Maria Luiza M</creator><creator>Sarkis, João José Freitas</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200206</creationdate><title>In vitro effects of thyroid hormones on ectonucleotidase activities in synaptosomes from hippocampus of rats</title><author>Matos, José Alfredo de Aguiar ; Bruno, Alessandra Nejar ; Oses, Jean Pierre ; Bonan, Carla Denise ; Battastini, Ana Maria Oliveira ; Barreto-Chaves, Maria Luiza M ; Sarkis, João José Freitas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-9c4a1f943d82f113c9c0472ab10244126f3732f6dab513a76b40b9987e2e1c9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenosine - metabolism</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Monophosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Extracellular Space - drug effects</topic><topic>Extracellular Space - metabolism</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - enzymology</topic><topic>Hydrolysis - drug effects</topic><topic>Male</topic><topic>Presynaptic Terminals - drug effects</topic><topic>Presynaptic Terminals - enzymology</topic><topic>Pyrophosphatases - drug effects</topic><topic>Pyrophosphatases - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Synaptosomes - drug effects</topic><topic>Synaptosomes - metabolism</topic><topic>Thyroid Gland - metabolism</topic><topic>Thyroid Hormones - metabolism</topic><topic>Thyroid Hormones - pharmacology</topic><topic>Thyroxine - metabolism</topic><topic>Thyroxine - pharmacology</topic><topic>Triiodothyronine - metabolism</topic><topic>Triiodothyronine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matos, José Alfredo de Aguiar</creatorcontrib><creatorcontrib>Bruno, Alessandra Nejar</creatorcontrib><creatorcontrib>Oses, Jean Pierre</creatorcontrib><creatorcontrib>Bonan, Carla Denise</creatorcontrib><creatorcontrib>Battastini, Ana Maria Oliveira</creatorcontrib><creatorcontrib>Barreto-Chaves, Maria Luiza M</creatorcontrib><creatorcontrib>Sarkis, João José Freitas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular and molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matos, José Alfredo de Aguiar</au><au>Bruno, Alessandra Nejar</au><au>Oses, Jean Pierre</au><au>Bonan, Carla Denise</au><au>Battastini, Ana Maria Oliveira</au><au>Barreto-Chaves, Maria Luiza M</au><au>Sarkis, João José Freitas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effects of thyroid hormones on ectonucleotidase activities in synaptosomes from hippocampus of rats</atitle><jtitle>Cellular and molecular neurobiology</jtitle><addtitle>Cell Mol Neurobiol</addtitle><date>2002-06</date><risdate>2002</risdate><volume>22</volume><issue>3</issue><spage>345</spage><epage>352</epage><pages>345-352</pages><issn>0272-4340</issn><abstract>1. Studies have shown that adenosine transport and adenosine A1 receptors in rat brain are subjected to regulation by thyroid hormone levels. Since the ectonucleotidase pathway is an important source of adenosine extracellular, in the present study the in vitro action of T3 and T4 hormones on ectonucleotidase activities in hippocampal synaptosomes was evaluated. 2. T3 (Triiodo-l-thyronine) significantly inhibited, in an uncompetitive manner, the ATP and ADP hydrolysis promoted by ATP diphosphohydrolase activity in hippocampal synaptosomes of adult rats. 3. In contrast, T4 (Thyroxine) only inhibited ATP hydrolysis in an uncompetitive mechanism, at the concentrations tested (100-500 microM), but at the same time did not affect ADP hydrolysis. 4. In the present study, we also investigate the in vitro effect of T3 and T4 on 5'-nucleotidase activity. However, there are no changes in the activity of this enzyme in the presence of T3 and T4 in the hippocampal synaptosomes of rats. 5. These results suggest that thyroid hormones could be involved in the regulation of ectonucleotidase activities, such as ecto-ATP diphosphohydrolase and ecto-ATPase, possibly exerting a modulatory role in extracellular adenosine levels.</abstract><cop>Netherlands</cop><pmid>12469875</pmid><doi>10.1023/A:1020776119612</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine - metabolism Adenosine Diphosphate - metabolism Adenosine Monophosphate - metabolism Adenosine Triphosphate - metabolism Animals Extracellular Space - drug effects Extracellular Space - metabolism Hippocampus - drug effects Hippocampus - enzymology Hydrolysis - drug effects Male Presynaptic Terminals - drug effects Presynaptic Terminals - enzymology Pyrophosphatases - drug effects Pyrophosphatases - metabolism Rats Rats, Wistar Synaptosomes - drug effects Synaptosomes - metabolism Thyroid Gland - metabolism Thyroid Hormones - metabolism Thyroid Hormones - pharmacology Thyroxine - metabolism Thyroxine - pharmacology Triiodothyronine - metabolism Triiodothyronine - pharmacology |
| title | In vitro effects of thyroid hormones on ectonucleotidase activities in synaptosomes from hippocampus of rats |
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