Detection of hepatitis C virus RNA in serum of patients with chronic hepatitis C treated with interferon‐α
We tested serial serum samples for hepatitis C virus RNA from patients undergoing treatment for chronic hepatitis C with interferon‐α using an assay that combined reverse transcription and polymerase chain reaction. The subjects studied were 20 patients with chronic hepatitis who had serum antibody...
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| Veröffentlicht in: | Hepatology (Baltimore, Md.) Md.), 1992-01, Vol.15 (1), p.37-41 |
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| container_title | Hepatology (Baltimore, Md.) |
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| creator | Hagiwara, Hideki Hayashi, Norio Mita, Eiji Ueda, Keiji Takehara, Tetsuo Kasahara, Akinori Fusamoto, Hideyuki Kamada, Takenobu |
| description | We tested serial serum samples for hepatitis C virus RNA from patients undergoing treatment for chronic hepatitis C with interferon‐α using an assay that combined reverse transcription and polymerase chain reaction. The subjects studied were 20 patients with chronic hepatitis who had serum antibody to hepatitis C virus (anti‐C100–3). Before therapy, hepatitis C virus RNA was detected in 18 (90%) and 20 (100%) patients using primer sets derived from the NS3 region or the 5′‐noncoding region of hepatitis C virus, respectively. Hepatitis C virus RNA became undetectable in all patients whose ALT level fell into the normal range during therapy. However, hepatitis C virus RNA reappeared in all patients whose ALT levels rose again after therapy, usually before the relapse. In patients whose ALT levels did not become normal, hepatitis C virus RNA did not disappear during therapy. Thus therapy with interferon‐α appears to be beneficial in chronic hepatitis C because of its suppressive effects on hepatitis C virus replication. Detection of hepatitis C virus RNA in serum is useful for evaluating the antiviral effect of interferon (HEPATOLOGY 1992;15:37–41). |
| doi_str_mv | 10.1002/hep.1840150108 |
| format | Article |
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The subjects studied were 20 patients with chronic hepatitis who had serum antibody to hepatitis C virus (anti‐C100–3). Before therapy, hepatitis C virus RNA was detected in 18 (90%) and 20 (100%) patients using primer sets derived from the NS3 region or the 5′‐noncoding region of hepatitis C virus, respectively. Hepatitis C virus RNA became undetectable in all patients whose ALT level fell into the normal range during therapy. However, hepatitis C virus RNA reappeared in all patients whose ALT levels rose again after therapy, usually before the relapse. In patients whose ALT levels did not become normal, hepatitis C virus RNA did not disappear during therapy. Thus therapy with interferon‐α appears to be beneficial in chronic hepatitis C because of its suppressive effects on hepatitis C virus replication. Detection of hepatitis C virus RNA in serum is useful for evaluating the antiviral effect of interferon (HEPATOLOGY 1992;15:37–41).</description><identifier>ISSN: 0270-9139</identifier><identifier>EISSN: 1527-3350</identifier><identifier>DOI: 10.1002/hep.1840150108</identifier><identifier>PMID: 1370161</identifier><identifier>CODEN: HPTLD9</identifier><language>eng</language><publisher>Philadelphia, PA: W.B. Saunders</publisher><subject>Adult ; Alanine Transaminase - blood ; Amino Acid Sequence ; Biological and medical sciences ; Chronic Disease ; Cohort Studies ; Female ; Hepacivirus - genetics ; Hepatitis Antibodies - analysis ; Hepatitis C - blood ; Hepatitis C - genetics ; Hepatitis C - therapy ; Hepatitis C Antibodies ; Humans ; Immunomodulators ; Interferon-alpha - therapeutic use ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Pharmacology. Drug treatments ; RNA, Viral - analysis</subject><ispartof>Hepatology (Baltimore, Md.), 1992-01, Vol.15 (1), p.37-41</ispartof><rights>Copyright © 1992 American Association for the Study of Liver Diseases</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4098-6466ba760d9af87a3f568958b27eb5ec05bfb1909e011d40e79fd88c7016e9583</citedby><cites>FETCH-LOGICAL-c4098-6466ba760d9af87a3f568958b27eb5ec05bfb1909e011d40e79fd88c7016e9583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fhep.1840150108$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fhep.1840150108$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4009,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5166118$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1370161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hagiwara, Hideki</creatorcontrib><creatorcontrib>Hayashi, Norio</creatorcontrib><creatorcontrib>Mita, Eiji</creatorcontrib><creatorcontrib>Ueda, Keiji</creatorcontrib><creatorcontrib>Takehara, Tetsuo</creatorcontrib><creatorcontrib>Kasahara, Akinori</creatorcontrib><creatorcontrib>Fusamoto, Hideyuki</creatorcontrib><creatorcontrib>Kamada, Takenobu</creatorcontrib><title>Detection of hepatitis C virus RNA in serum of patients with chronic hepatitis C treated with interferon‐α</title><title>Hepatology (Baltimore, Md.)</title><addtitle>Hepatology</addtitle><description>We tested serial serum samples for hepatitis C virus RNA from patients undergoing treatment for chronic hepatitis C with interferon‐α using an assay that combined reverse transcription and polymerase chain reaction. The subjects studied were 20 patients with chronic hepatitis who had serum antibody to hepatitis C virus (anti‐C100–3). Before therapy, hepatitis C virus RNA was detected in 18 (90%) and 20 (100%) patients using primer sets derived from the NS3 region or the 5′‐noncoding region of hepatitis C virus, respectively. Hepatitis C virus RNA became undetectable in all patients whose ALT level fell into the normal range during therapy. However, hepatitis C virus RNA reappeared in all patients whose ALT levels rose again after therapy, usually before the relapse. In patients whose ALT levels did not become normal, hepatitis C virus RNA did not disappear during therapy. Thus therapy with interferon‐α appears to be beneficial in chronic hepatitis C because of its suppressive effects on hepatitis C virus replication. Detection of hepatitis C virus RNA in serum is useful for evaluating the antiviral effect of interferon (HEPATOLOGY 1992;15:37–41).</description><subject>Adult</subject><subject>Alanine Transaminase - blood</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Chronic Disease</subject><subject>Cohort Studies</subject><subject>Female</subject><subject>Hepacivirus - genetics</subject><subject>Hepatitis Antibodies - analysis</subject><subject>Hepatitis C - blood</subject><subject>Hepatitis C - genetics</subject><subject>Hepatitis C - therapy</subject><subject>Hepatitis C Antibodies</subject><subject>Humans</subject><subject>Immunomodulators</subject><subject>Interferon-alpha - therapeutic use</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Pharmacology. Drug treatments</subject><subject>RNA, Viral - analysis</subject><issn>0270-9139</issn><issn>1527-3350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1u1EAQhVsIFIbAlh1SLxA7D1X-6Z9lNCQEKQKEYG2129WaRv4ZutuJsuMIXCUX4RCcBFseEVixqsX76lW9x9hzhC0C5K_3dNiiKgErQFAP2AarXGZFUcFDtoFcQqax0I_Zkxi_AoAuc3XCTrCQgAI3rH9DiWzy48BHx2czk3zyke_4tQ9T5J_en3E_8Ehh6hdi0WlIkd_4tOd2H8bB23_2UiCTqF0BPyQKjmbq1_cfP--eskfOdJGeHecp-3Jx_nl3mV19ePtud3aV2RK0ykQpRGOkgFYbp6QpXCWUrlSTS2oqslA1rkENmgCxLYGkdq1SdslEM1ecsler7yGM3yaKqe59tNR1ZqBxirXMZanzQszgdgVtGGMM5OpD8L0JtzVCvfRbz9Hq-37nhRdH56npqb3H10Jn_eVRN9GazgUzWB__YBUKgbjY6BW78R3d_udofXn-8a8XfgMispUu</recordid><startdate>199201</startdate><enddate>199201</enddate><creator>Hagiwara, Hideki</creator><creator>Hayashi, Norio</creator><creator>Mita, Eiji</creator><creator>Ueda, Keiji</creator><creator>Takehara, Tetsuo</creator><creator>Kasahara, Akinori</creator><creator>Fusamoto, Hideyuki</creator><creator>Kamada, Takenobu</creator><general>W.B. Saunders</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199201</creationdate><title>Detection of hepatitis C virus RNA in serum of patients with chronic hepatitis C treated with interferon‐α</title><author>Hagiwara, Hideki ; Hayashi, Norio ; Mita, Eiji ; Ueda, Keiji ; Takehara, Tetsuo ; Kasahara, Akinori ; Fusamoto, Hideyuki ; Kamada, Takenobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4098-6466ba760d9af87a3f568958b27eb5ec05bfb1909e011d40e79fd88c7016e9583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Alanine Transaminase - blood</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Chronic Disease</topic><topic>Cohort Studies</topic><topic>Female</topic><topic>Hepacivirus - genetics</topic><topic>Hepatitis Antibodies - analysis</topic><topic>Hepatitis C - blood</topic><topic>Hepatitis C - genetics</topic><topic>Hepatitis C - therapy</topic><topic>Hepatitis C Antibodies</topic><topic>Humans</topic><topic>Immunomodulators</topic><topic>Interferon-alpha - therapeutic use</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Pharmacology. Drug treatments</topic><topic>RNA, Viral - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hagiwara, Hideki</creatorcontrib><creatorcontrib>Hayashi, Norio</creatorcontrib><creatorcontrib>Mita, Eiji</creatorcontrib><creatorcontrib>Ueda, Keiji</creatorcontrib><creatorcontrib>Takehara, Tetsuo</creatorcontrib><creatorcontrib>Kasahara, Akinori</creatorcontrib><creatorcontrib>Fusamoto, Hideyuki</creatorcontrib><creatorcontrib>Kamada, Takenobu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hepatology (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hagiwara, Hideki</au><au>Hayashi, Norio</au><au>Mita, Eiji</au><au>Ueda, Keiji</au><au>Takehara, Tetsuo</au><au>Kasahara, Akinori</au><au>Fusamoto, Hideyuki</au><au>Kamada, Takenobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of hepatitis C virus RNA in serum of patients with chronic hepatitis C treated with interferon‐α</atitle><jtitle>Hepatology (Baltimore, Md.)</jtitle><addtitle>Hepatology</addtitle><date>1992-01</date><risdate>1992</risdate><volume>15</volume><issue>1</issue><spage>37</spage><epage>41</epage><pages>37-41</pages><issn>0270-9139</issn><eissn>1527-3350</eissn><coden>HPTLD9</coden><abstract>We tested serial serum samples for hepatitis C virus RNA from patients undergoing treatment for chronic hepatitis C with interferon‐α using an assay that combined reverse transcription and polymerase chain reaction. The subjects studied were 20 patients with chronic hepatitis who had serum antibody to hepatitis C virus (anti‐C100–3). Before therapy, hepatitis C virus RNA was detected in 18 (90%) and 20 (100%) patients using primer sets derived from the NS3 region or the 5′‐noncoding region of hepatitis C virus, respectively. Hepatitis C virus RNA became undetectable in all patients whose ALT level fell into the normal range during therapy. However, hepatitis C virus RNA reappeared in all patients whose ALT levels rose again after therapy, usually before the relapse. In patients whose ALT levels did not become normal, hepatitis C virus RNA did not disappear during therapy. Thus therapy with interferon‐α appears to be beneficial in chronic hepatitis C because of its suppressive effects on hepatitis C virus replication. Detection of hepatitis C virus RNA in serum is useful for evaluating the antiviral effect of interferon (HEPATOLOGY 1992;15:37–41).</abstract><cop>Philadelphia, PA</cop><pub>W.B. 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| ispartof | Hepatology (Baltimore, Md.), 1992-01, Vol.15 (1), p.37-41 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Alanine Transaminase - blood Amino Acid Sequence Biological and medical sciences Chronic Disease Cohort Studies Female Hepacivirus - genetics Hepatitis Antibodies - analysis Hepatitis C - blood Hepatitis C - genetics Hepatitis C - therapy Hepatitis C Antibodies Humans Immunomodulators Interferon-alpha - therapeutic use Male Medical sciences Middle Aged Molecular Sequence Data Pharmacology. Drug treatments RNA, Viral - analysis |
| title | Detection of hepatitis C virus RNA in serum of patients with chronic hepatitis C treated with interferon‐α |
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