Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis
Early changes in parathyroid hormone-related peptide (PTH-rP) and Indian hedgehog (Ihh) expression were examined in equine articular osteochondrosis (OC) as a model of a naturally acquired dyschondroplasia. Cartilage was harvested from OC-affected femoropatellar or scapulohumeral joints from immatur...
Gespeichert in:
| Veröffentlicht in: | Journal of orthopaedic research 2002-11, Vol.20 (6), p.1290-1297 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1297 |
|---|---|
| container_issue | 6 |
| container_start_page | 1290 |
| container_title | Journal of orthopaedic research |
| container_volume | 20 |
| creator | Semevolos, Stacy A Brower-Toland, Brent D Bent, Stephen J Nixon, Alan J |
| description | Early changes in parathyroid hormone-related peptide (PTH-rP) and Indian hedgehog (Ihh) expression were examined in equine articular osteochondrosis (OC) as a model of a naturally acquired dyschondroplasia. Cartilage was harvested from OC-affected femoropatellar or scapulohumeral joints from immature horses and normal control horses of similar age. PTH-rP expression levels were assessed by semi-quantitative PCR, in situ hybridization, and immunohistochemistry. Ihh protein expression levels were assessed by immunohistochemistry. Elevated PTH-rP protein and mRNA expression were identified in the deeper layers of affected articular cartilage and the fibrous tissue of interposing clefts. These changes were confined to the chondrocytes in the OC-affected cartilage, which had significantly increased PTH-rP protein and mRNA expression when compared to control cartilages. Ihh protein expression showed similar distribution as PTH-rP in the deeper layers of articular cartilage; however, only a trend for increased Ihh immunostaining was evident in the OC cartilage when compared to the normal cartilage. Increased PTH-rP expression in pre-hypertrophic chondrocytes of diseased OC cartilage suggests a possible link between this peptide and the delayed ossification, which is a consistent histologic alteration in OC. More evidence is necessary to determine the role of Ihh in articular cartilage and if a similar feedback cycle exists as previously described for the growth plate. |
| doi_str_mv | 10.1016/S0736-0266(02)00055-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72746642</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0736026602000554</els_id><sourcerecordid>18638619</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5614-82eb0150f0d6dfb7d27ab32c0c6cb502e07c66871267d5028e50ec875b0e298d3</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhiMEotvCTwBZHFA5BMZObKenggqUwqot39wsx57tumTt1E5g99_j7a6KxKVcPLL0zDt69RTFIwrPKVDx4jPISpTAhNgH9gwAOC_rO8WEcl6XnMkfd4vJDbJT7KZ0mSFJWXO_2KGslozVbFIsz3XUw3wVg7NkHuIieCwjdnpAS3rsB2eRaG_JibdOezJHe4HzcEFw2UdMyQVPej0MGH0izhOvhzHqrlsRba5GF3MK5umRhDRgMPPgbQzJpQfFvZnuEj7czr3i69s3X47eldOz45OjV9PScEHrsmHYAuUwAyvsrJWWSd1WzIARpuXAEKQRosm9hLT53yAHNI3kLSA7aGy1Vzzd5PYxXI2YBrVwyWDXaY9hTEoyWQtRs1tB2oiqEfQgg0_-AS_DGH0uoVjFKYhsIEN8A5ncNkWcqT66hY4rRUGtBaprgWptJz_qWqBa7z3eho_tAu3fra2xDLzcAL9dh6v_S1Xvzz5RCsAABFvfKDcRLitZ3kTo-FMJWUmuvp8eq1P-Yfr6W_1RnWf-cMNj9vTLYVTJOPQGbfZrBmWDu6XVH09_zYI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>235106554</pqid></control><display><type>article</type><title>Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Wiley Online Library (Open Access Collection)</source><source>Alma/SFX Local Collection</source><creator>Semevolos, Stacy A ; Brower-Toland, Brent D ; Bent, Stephen J ; Nixon, Alan J</creator><creatorcontrib>Semevolos, Stacy A ; Brower-Toland, Brent D ; Bent, Stephen J ; Nixon, Alan J</creatorcontrib><description>Early changes in parathyroid hormone-related peptide (PTH-rP) and Indian hedgehog (Ihh) expression were examined in equine articular osteochondrosis (OC) as a model of a naturally acquired dyschondroplasia. Cartilage was harvested from OC-affected femoropatellar or scapulohumeral joints from immature horses and normal control horses of similar age. PTH-rP expression levels were assessed by semi-quantitative PCR, in situ hybridization, and immunohistochemistry. Ihh protein expression levels were assessed by immunohistochemistry. Elevated PTH-rP protein and mRNA expression were identified in the deeper layers of affected articular cartilage and the fibrous tissue of interposing clefts. These changes were confined to the chondrocytes in the OC-affected cartilage, which had significantly increased PTH-rP protein and mRNA expression when compared to control cartilages. Ihh protein expression showed similar distribution as PTH-rP in the deeper layers of articular cartilage; however, only a trend for increased Ihh immunostaining was evident in the OC cartilage when compared to the normal cartilage. Increased PTH-rP expression in pre-hypertrophic chondrocytes of diseased OC cartilage suggests a possible link between this peptide and the delayed ossification, which is a consistent histologic alteration in OC. More evidence is necessary to determine the role of Ihh in articular cartilage and if a similar feedback cycle exists as previously described for the growth plate.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1016/S0736-0266(02)00055-4</identifier><identifier>PMID: 12472242</identifier><identifier>CODEN: JOREDR</identifier><language>eng</language><publisher>Hoboken: Elsevier Ltd</publisher><subject>Animals ; Cartilage, Articular - physiopathology ; Development ; Disease Models, Animal ; Dyschondroplasia ; Gene Expression ; Hedgehog Proteins ; Horse Diseases - physiopathology ; Horses ; Hypertrophy ; Ihh ; Osteochondritis - physiopathology ; Osteochondrodysplasias - physiopathology ; Osteochondrosis ; Parathyroid Hormone-Related Protein ; Peptide Hormones - genetics ; PTH-rP ; RNA, Messenger - analysis ; Trans-Activators - genetics</subject><ispartof>Journal of orthopaedic research, 2002-11, Vol.20 (6), p.1290-1297</ispartof><rights>2002 Orthopaedic Research Society</rights><rights>Copyright © 2002 Orthopaedic Research Society</rights><rights>Copyright Journal of Bone and Joint Surgery, Inc. Nov 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5614-82eb0150f0d6dfb7d27ab32c0c6cb502e07c66871267d5028e50ec875b0e298d3</citedby><cites>FETCH-LOGICAL-c5614-82eb0150f0d6dfb7d27ab32c0c6cb502e07c66871267d5028e50ec875b0e298d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0736-0266%2802%2900055-4$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0736-0266%2802%2900055-4$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12472242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Semevolos, Stacy A</creatorcontrib><creatorcontrib>Brower-Toland, Brent D</creatorcontrib><creatorcontrib>Bent, Stephen J</creatorcontrib><creatorcontrib>Nixon, Alan J</creatorcontrib><title>Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>Early changes in parathyroid hormone-related peptide (PTH-rP) and Indian hedgehog (Ihh) expression were examined in equine articular osteochondrosis (OC) as a model of a naturally acquired dyschondroplasia. Cartilage was harvested from OC-affected femoropatellar or scapulohumeral joints from immature horses and normal control horses of similar age. PTH-rP expression levels were assessed by semi-quantitative PCR, in situ hybridization, and immunohistochemistry. Ihh protein expression levels were assessed by immunohistochemistry. Elevated PTH-rP protein and mRNA expression were identified in the deeper layers of affected articular cartilage and the fibrous tissue of interposing clefts. These changes were confined to the chondrocytes in the OC-affected cartilage, which had significantly increased PTH-rP protein and mRNA expression when compared to control cartilages. Ihh protein expression showed similar distribution as PTH-rP in the deeper layers of articular cartilage; however, only a trend for increased Ihh immunostaining was evident in the OC cartilage when compared to the normal cartilage. Increased PTH-rP expression in pre-hypertrophic chondrocytes of diseased OC cartilage suggests a possible link between this peptide and the delayed ossification, which is a consistent histologic alteration in OC. More evidence is necessary to determine the role of Ihh in articular cartilage and if a similar feedback cycle exists as previously described for the growth plate.</description><subject>Animals</subject><subject>Cartilage, Articular - physiopathology</subject><subject>Development</subject><subject>Disease Models, Animal</subject><subject>Dyschondroplasia</subject><subject>Gene Expression</subject><subject>Hedgehog Proteins</subject><subject>Horse Diseases - physiopathology</subject><subject>Horses</subject><subject>Hypertrophy</subject><subject>Ihh</subject><subject>Osteochondritis - physiopathology</subject><subject>Osteochondrodysplasias - physiopathology</subject><subject>Osteochondrosis</subject><subject>Parathyroid Hormone-Related Protein</subject><subject>Peptide Hormones - genetics</subject><subject>PTH-rP</subject><subject>RNA, Messenger - analysis</subject><subject>Trans-Activators - genetics</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1v1DAQhiMEotvCTwBZHFA5BMZObKenggqUwqot39wsx57tumTt1E5g99_j7a6KxKVcPLL0zDt69RTFIwrPKVDx4jPISpTAhNgH9gwAOC_rO8WEcl6XnMkfd4vJDbJT7KZ0mSFJWXO_2KGslozVbFIsz3XUw3wVg7NkHuIieCwjdnpAS3rsB2eRaG_JibdOezJHe4HzcEFw2UdMyQVPej0MGH0izhOvhzHqrlsRba5GF3MK5umRhDRgMPPgbQzJpQfFvZnuEj7czr3i69s3X47eldOz45OjV9PScEHrsmHYAuUwAyvsrJWWSd1WzIARpuXAEKQRosm9hLT53yAHNI3kLSA7aGy1Vzzd5PYxXI2YBrVwyWDXaY9hTEoyWQtRs1tB2oiqEfQgg0_-AS_DGH0uoVjFKYhsIEN8A5ncNkWcqT66hY4rRUGtBaprgWptJz_qWqBa7z3eho_tAu3fra2xDLzcAL9dh6v_S1Xvzz5RCsAABFvfKDcRLitZ3kTo-FMJWUmuvp8eq1P-Yfr6W_1RnWf-cMNj9vTLYVTJOPQGbfZrBmWDu6XVH09_zYI</recordid><startdate>200211</startdate><enddate>200211</enddate><creator>Semevolos, Stacy A</creator><creator>Brower-Toland, Brent D</creator><creator>Bent, Stephen J</creator><creator>Nixon, Alan J</creator><general>Elsevier Ltd</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200211</creationdate><title>Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis</title><author>Semevolos, Stacy A ; Brower-Toland, Brent D ; Bent, Stephen J ; Nixon, Alan J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5614-82eb0150f0d6dfb7d27ab32c0c6cb502e07c66871267d5028e50ec875b0e298d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cartilage, Articular - physiopathology</topic><topic>Development</topic><topic>Disease Models, Animal</topic><topic>Dyschondroplasia</topic><topic>Gene Expression</topic><topic>Hedgehog Proteins</topic><topic>Horse Diseases - physiopathology</topic><topic>Horses</topic><topic>Hypertrophy</topic><topic>Ihh</topic><topic>Osteochondritis - physiopathology</topic><topic>Osteochondrodysplasias - physiopathology</topic><topic>Osteochondrosis</topic><topic>Parathyroid Hormone-Related Protein</topic><topic>Peptide Hormones - genetics</topic><topic>PTH-rP</topic><topic>RNA, Messenger - analysis</topic><topic>Trans-Activators - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Semevolos, Stacy A</creatorcontrib><creatorcontrib>Brower-Toland, Brent D</creatorcontrib><creatorcontrib>Bent, Stephen J</creatorcontrib><creatorcontrib>Nixon, Alan J</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Semevolos, Stacy A</au><au>Brower-Toland, Brent D</au><au>Bent, Stephen J</au><au>Nixon, Alan J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2002-11</date><risdate>2002</risdate><volume>20</volume><issue>6</issue><spage>1290</spage><epage>1297</epage><pages>1290-1297</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><coden>JOREDR</coden><abstract>Early changes in parathyroid hormone-related peptide (PTH-rP) and Indian hedgehog (Ihh) expression were examined in equine articular osteochondrosis (OC) as a model of a naturally acquired dyschondroplasia. Cartilage was harvested from OC-affected femoropatellar or scapulohumeral joints from immature horses and normal control horses of similar age. PTH-rP expression levels were assessed by semi-quantitative PCR, in situ hybridization, and immunohistochemistry. Ihh protein expression levels were assessed by immunohistochemistry. Elevated PTH-rP protein and mRNA expression were identified in the deeper layers of affected articular cartilage and the fibrous tissue of interposing clefts. These changes were confined to the chondrocytes in the OC-affected cartilage, which had significantly increased PTH-rP protein and mRNA expression when compared to control cartilages. Ihh protein expression showed similar distribution as PTH-rP in the deeper layers of articular cartilage; however, only a trend for increased Ihh immunostaining was evident in the OC cartilage when compared to the normal cartilage. Increased PTH-rP expression in pre-hypertrophic chondrocytes of diseased OC cartilage suggests a possible link between this peptide and the delayed ossification, which is a consistent histologic alteration in OC. More evidence is necessary to determine the role of Ihh in articular cartilage and if a similar feedback cycle exists as previously described for the growth plate.</abstract><cop>Hoboken</cop><pub>Elsevier Ltd</pub><pmid>12472242</pmid><doi>10.1016/S0736-0266(02)00055-4</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0736-0266 |
| ispartof | Journal of orthopaedic research, 2002-11, Vol.20 (6), p.1290-1297 |
| issn | 0736-0266 1554-527X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72746642 |
| source | MEDLINE; Access via Wiley Online Library; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Animals Cartilage, Articular - physiopathology Development Disease Models, Animal Dyschondroplasia Gene Expression Hedgehog Proteins Horse Diseases - physiopathology Horses Hypertrophy Ihh Osteochondritis - physiopathology Osteochondrodysplasias - physiopathology Osteochondrosis Parathyroid Hormone-Related Protein Peptide Hormones - genetics PTH-rP RNA, Messenger - analysis Trans-Activators - genetics |
| title | Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T17%3A42%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Parathyroid%20hormone-related%20peptide%20and%20Indian%20hedgehog%20expression%20patterns%20in%20naturally%20acquired%20equine%20osteochondrosis&rft.jtitle=Journal%20of%20orthopaedic%20research&rft.au=Semevolos,%20Stacy%20A&rft.date=2002-11&rft.volume=20&rft.issue=6&rft.spage=1290&rft.epage=1297&rft.pages=1290-1297&rft.issn=0736-0266&rft.eissn=1554-527X&rft.coden=JOREDR&rft_id=info:doi/10.1016/S0736-0266(02)00055-4&rft_dat=%3Cproquest_cross%3E18638619%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=235106554&rft_id=info:pmid/12472242&rft_els_id=S0736026602000554&rfr_iscdi=true |