Guidance of Primordial Germ Cell Migration by the Chemokine SDF-1
The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1...
Gespeichert in:
| Veröffentlicht in: | Cell 2002-11, Vol.111 (5), p.647-659 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 659 |
|---|---|
| container_issue | 5 |
| container_start_page | 647 |
| container_title | Cell |
| container_volume | 111 |
| creator | Doitsidou, Maria Reichman-Fried, Michal Stebler, Juürg Köprunner, Marion Dörries, Julia Meyer, Dirk Esguerra, Camila V. Leung, TinChung Raz, Erez |
| description | The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that
sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs. |
| doi_str_mv | 10.1016/S0092-8674(02)01135-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72742936</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0092867402011352</els_id><sourcerecordid>72742936</sourcerecordid><originalsourceid>FETCH-LOGICAL-c557t-464b8c4d0ae36182553d88ab2aa216906e172c759f770203e2840e4a5d03684e3</originalsourceid><addsrcrecordid>eNqFkMtOwzAQRS0EouXxCSCvECwCY8evrFBVaEECgQSsLSeZgKFpwE6R-HsMrWDZ1WzOnXt1CDlgcMqAqbMHgIJnRmlxDPwEGMtlxjfIkEGhM8E03yTDP2RAdmJ8BQAjpdwmA8aFSowektF04Ws3r5B2Db0Pvu1C7d2MTjG0dIyzGb31z8H1vpvT8ov2L0jHL9h2b36O9OFikrE9stW4WcT91d0lT5PLx_FVdnM3vR6PbrJKSt1nqbA0lajBYa6Y4VLmtTGu5M5xpgpQmDZXWhaN1sAhR24EoHCyhlwZgfkuOVr-fQ_dxwJjb1sfq7TQzbFbRKu5FrzI1VqQGSULw0UC5RKsQhdjwMa-JwEufFkG9key_ZVsfwxa4PZXsuUpd7gqWJQt1v-pldUEnC8BTD4-PQYbK49Jcu0DVr2tO7-m4hv7S4hI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18659824</pqid></control><display><type>article</type><title>Guidance of Primordial Germ Cell Migration by the Chemokine SDF-1</title><source>MEDLINE</source><source>Cell Press Free Archives</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Doitsidou, Maria ; Reichman-Fried, Michal ; Stebler, Juürg ; Köprunner, Marion ; Dörries, Julia ; Meyer, Dirk ; Esguerra, Camila V. ; Leung, TinChung ; Raz, Erez</creator><creatorcontrib>Doitsidou, Maria ; Reichman-Fried, Michal ; Stebler, Juürg ; Köprunner, Marion ; Dörries, Julia ; Meyer, Dirk ; Esguerra, Camila V. ; Leung, TinChung ; Raz, Erez</creatorcontrib><description>The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that
sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/S0092-8674(02)01135-2</identifier><identifier>PMID: 12464177</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Body Patterning ; Cell Movement ; Cell Polarity ; Chemokine CXCL12 ; Chemokines, CXC - metabolism ; Embryo, Nonmammalian - cytology ; Embryo, Nonmammalian - embryology ; Gene Expression Regulation, Developmental ; Germ Cells - cytology ; Germ Cells - physiology ; Mutation ; Oligonucleotides, Antisense - genetics ; Protein Biosynthesis ; Receptors, CXCR4 - metabolism ; RNA, Messenger - metabolism ; Signal Transduction ; Tissue Distribution ; Zebrafish</subject><ispartof>Cell, 2002-11, Vol.111 (5), p.647-659</ispartof><rights>2002 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-464b8c4d0ae36182553d88ab2aa216906e172c759f770203e2840e4a5d03684e3</citedby><cites>FETCH-LOGICAL-c557t-464b8c4d0ae36182553d88ab2aa216906e172c759f770203e2840e4a5d03684e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0092-8674(02)01135-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12464177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doitsidou, Maria</creatorcontrib><creatorcontrib>Reichman-Fried, Michal</creatorcontrib><creatorcontrib>Stebler, Juürg</creatorcontrib><creatorcontrib>Köprunner, Marion</creatorcontrib><creatorcontrib>Dörries, Julia</creatorcontrib><creatorcontrib>Meyer, Dirk</creatorcontrib><creatorcontrib>Esguerra, Camila V.</creatorcontrib><creatorcontrib>Leung, TinChung</creatorcontrib><creatorcontrib>Raz, Erez</creatorcontrib><title>Guidance of Primordial Germ Cell Migration by the Chemokine SDF-1</title><title>Cell</title><addtitle>Cell</addtitle><description>The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that
sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.</description><subject>Animals</subject><subject>Body Patterning</subject><subject>Cell Movement</subject><subject>Cell Polarity</subject><subject>Chemokine CXCL12</subject><subject>Chemokines, CXC - metabolism</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryo, Nonmammalian - embryology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - physiology</subject><subject>Mutation</subject><subject>Oligonucleotides, Antisense - genetics</subject><subject>Protein Biosynthesis</subject><subject>Receptors, CXCR4 - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction</subject><subject>Tissue Distribution</subject><subject>Zebrafish</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EouXxCSCvECwCY8evrFBVaEECgQSsLSeZgKFpwE6R-HsMrWDZ1WzOnXt1CDlgcMqAqbMHgIJnRmlxDPwEGMtlxjfIkEGhM8E03yTDP2RAdmJ8BQAjpdwmA8aFSowektF04Ws3r5B2Db0Pvu1C7d2MTjG0dIyzGb31z8H1vpvT8ov2L0jHL9h2b36O9OFikrE9stW4WcT91d0lT5PLx_FVdnM3vR6PbrJKSt1nqbA0lajBYa6Y4VLmtTGu5M5xpgpQmDZXWhaN1sAhR24EoHCyhlwZgfkuOVr-fQ_dxwJjb1sfq7TQzbFbRKu5FrzI1VqQGSULw0UC5RKsQhdjwMa-JwEufFkG9key_ZVsfwxa4PZXsuUpd7gqWJQt1v-pldUEnC8BTD4-PQYbK49Jcu0DVr2tO7-m4hv7S4hI</recordid><startdate>20021127</startdate><enddate>20021127</enddate><creator>Doitsidou, Maria</creator><creator>Reichman-Fried, Michal</creator><creator>Stebler, Juürg</creator><creator>Köprunner, Marion</creator><creator>Dörries, Julia</creator><creator>Meyer, Dirk</creator><creator>Esguerra, Camila V.</creator><creator>Leung, TinChung</creator><creator>Raz, Erez</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20021127</creationdate><title>Guidance of Primordial Germ Cell Migration by the Chemokine SDF-1</title><author>Doitsidou, Maria ; Reichman-Fried, Michal ; Stebler, Juürg ; Köprunner, Marion ; Dörries, Julia ; Meyer, Dirk ; Esguerra, Camila V. ; Leung, TinChung ; Raz, Erez</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-464b8c4d0ae36182553d88ab2aa216906e172c759f770203e2840e4a5d03684e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Body Patterning</topic><topic>Cell Movement</topic><topic>Cell Polarity</topic><topic>Chemokine CXCL12</topic><topic>Chemokines, CXC - metabolism</topic><topic>Embryo, Nonmammalian - cytology</topic><topic>Embryo, Nonmammalian - embryology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - physiology</topic><topic>Mutation</topic><topic>Oligonucleotides, Antisense - genetics</topic><topic>Protein Biosynthesis</topic><topic>Receptors, CXCR4 - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction</topic><topic>Tissue Distribution</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doitsidou, Maria</creatorcontrib><creatorcontrib>Reichman-Fried, Michal</creatorcontrib><creatorcontrib>Stebler, Juürg</creatorcontrib><creatorcontrib>Köprunner, Marion</creatorcontrib><creatorcontrib>Dörries, Julia</creatorcontrib><creatorcontrib>Meyer, Dirk</creatorcontrib><creatorcontrib>Esguerra, Camila V.</creatorcontrib><creatorcontrib>Leung, TinChung</creatorcontrib><creatorcontrib>Raz, Erez</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doitsidou, Maria</au><au>Reichman-Fried, Michal</au><au>Stebler, Juürg</au><au>Köprunner, Marion</au><au>Dörries, Julia</au><au>Meyer, Dirk</au><au>Esguerra, Camila V.</au><au>Leung, TinChung</au><au>Raz, Erez</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guidance of Primordial Germ Cell Migration by the Chemokine SDF-1</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2002-11-27</date><risdate>2002</risdate><volume>111</volume><issue>5</issue><spage>647</spage><epage>659</epage><pages>647-659</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that
sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12464177</pmid><doi>10.1016/S0092-8674(02)01135-2</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 2002-11, Vol.111 (5), p.647-659 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72742936 |
| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Body Patterning Cell Movement Cell Polarity Chemokine CXCL12 Chemokines, CXC - metabolism Embryo, Nonmammalian - cytology Embryo, Nonmammalian - embryology Gene Expression Regulation, Developmental Germ Cells - cytology Germ Cells - physiology Mutation Oligonucleotides, Antisense - genetics Protein Biosynthesis Receptors, CXCR4 - metabolism RNA, Messenger - metabolism Signal Transduction Tissue Distribution Zebrafish |
| title | Guidance of Primordial Germ Cell Migration by the Chemokine SDF-1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T22%3A19%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Guidance%20of%20Primordial%20Germ%20Cell%20Migration%20by%20the%20Chemokine%20SDF-1&rft.jtitle=Cell&rft.au=Doitsidou,%20Maria&rft.date=2002-11-27&rft.volume=111&rft.issue=5&rft.spage=647&rft.epage=659&rft.pages=647-659&rft.issn=0092-8674&rft.eissn=1097-4172&rft_id=info:doi/10.1016/S0092-8674(02)01135-2&rft_dat=%3Cproquest_cross%3E72742936%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18659824&rft_id=info:pmid/12464177&rft_els_id=S0092867402011352&rfr_iscdi=true |