New rapid staining methods of Cryptosporidium oocysts in stools
Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between...
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Veröffentlicht in: | The Journal of protozoology 1991-11, Vol.38 (6), p.212S-214S |
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container_title | The Journal of protozoology |
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creator | Chichino, G Bruno, A Cevini, C Atzori, C Gatti, S Scaglia, M |
description | Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis. |
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A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. 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A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis.</abstract><cop>United States</cop><pmid>1726326</pmid></addata></record> |
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subjects | Acquired Immunodeficiency Syndrome - parasitology AIDS/HIV Animals Cryptosporidium - isolation & purification Evaluation Studies as Topic Feces - parasitology HIV Seropositivity - parasitology Humans Male Merbromin Sensitivity and Specificity Staining and Labeling - methods |
title | New rapid staining methods of Cryptosporidium oocysts in stools |
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