Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis
Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase from Trichoderma viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction, that is always observed...
Gespeichert in:
| Veröffentlicht in: | Applied biochemistry and biotechnology 1991-12, Vol.31 (3), p.253-266 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 266 |
|---|---|
| container_issue | 3 |
| container_start_page | 253 |
| container_title | Applied biochemistry and biotechnology |
| container_volume | 31 |
| creator | Ooshima, H. (Osaka City University, Osaka, Japan) Kurakake, M Kato, J Harano, Y |
| description | Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase from Trichoderma viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction, that is always observed in enzymatic hydrolysis of cellulose, could be explained by a decline of specific activity of adsorbed enzyme, and it was implied that the decline results from a loss of synergistic action between endoglucanase and exoglucanase. An empirical equation expresses the change of hydrolysis rate during the reaction and also shows that the change of the hydrolysis rate is caused by the decline of the specific enzymatic activity of adsorbed enzyme. |
| doi_str_mv | 10.1007/BF02921752 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72734206</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72734206</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-1ecfa25bec3c7cb9583a711c35046601287aa368656254c1cd0e05d7d9d43d133</originalsourceid><addsrcrecordid>eNqF0M9LHTEQB_BQFPvUXnosFHIoHgprM8nm19GKrwqCB6vXZV6Sfabs22iyK2z_-u7jPerR0zAzH4bhS8hnYOfAmP7xc8m45aAl_0AWIKWt5h4OyIJxLSrOjf1Ijkv5wxhwI_UROQIDRgqzII9X_d9pg0N0FN0QX-Mw0dRSF7pu7LAEir6kvAqepn4_Tdtp72kcCnVP2K8D9WOO_Zo-TT6nbiqxnJLDFrsSPu3rCXlYXv2-vK5u737dXF7cVk4oGCoIrkUuV8EJp93KSiNQAzghWa3U9luNKJRRUnFZO3CeBSa99tbXwoMQJ-Rsd_c5p5cxlKHZxLJ9E_uQxtLoOYCaM_UuBMWVZaae4fcddDmVkkPbPOe4wTw1wJpt2s1b2jP-ur86rjbBv9FdvPP-236PxWHXZuxdLP-Z5EwqrWf2ZcdaTA2u80we7i1YMBbEP9BVji0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16269084</pqid></control><display><type>article</type><title>Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ooshima, H. (Osaka City University, Osaka, Japan) ; Kurakake, M ; Kato, J ; Harano, Y</creator><creatorcontrib>Ooshima, H. (Osaka City University, Osaka, Japan) ; Kurakake, M ; Kato, J ; Harano, Y</creatorcontrib><description>Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase from Trichoderma viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction, that is always observed in enzymatic hydrolysis of cellulose, could be explained by a decline of specific activity of adsorbed enzyme, and it was implied that the decline results from a loss of synergistic action between endoglucanase and exoglucanase. An empirical equation expresses the change of hydrolysis rate during the reaction and also shows that the change of the hydrolysis rate is caused by the decline of the specific enzymatic activity of adsorbed enzyme.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/BF02921752</identifier><identifier>PMID: 1818538</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ADSORCION ; ADSORPTION ; avicel ; BETA-GLUCANASE ; BETA-GLUCOSIDASE ; beta-Glucosidase - metabolism ; Biological and medical sciences ; Biotechnology ; CELLULASE ; Cellulase - drug effects ; Cellulase - metabolism ; CELLULOSE ; Cellulose - chemistry ; Cellulose - metabolism ; CELLULOSES ; CELULASA ; CELULOSA ; endoglucanase ; enzymatic activity ; Enzyme engineering ; enzyme inactivation ; ENZYMIC ACTIVITY ; exoglucanase ; Fundamental and applied biological sciences. Psychology ; GLICOSIDASAS ; Glucan 1,3-beta-Glucosidase ; Glucose - pharmacology ; GLUCOSIDASA ; GLUCOSIDASE ; GLUCOSIDASES ; GLYCOSIDASE ; GLYCOSIDASES ; HIDROLISIS ; HYDROLYSE ; HYDROLYSIS ; INHIBICION ; INHIBITION ; Kinetics ; Methods. Procedures. Technologies ; Miscellaneous ; Protein Denaturation ; SINERGISMO ; SYNERGISM ; SYNERGISME ; TRICHODERMA ; Trichoderma - enzymology ; TRICHODERMA VIRIDE</subject><ispartof>Applied biochemistry and biotechnology, 1991-12, Vol.31 (3), p.253-266</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-1ecfa25bec3c7cb9583a711c35046601287aa368656254c1cd0e05d7d9d43d133</citedby><cites>FETCH-LOGICAL-c361t-1ecfa25bec3c7cb9583a711c35046601287aa368656254c1cd0e05d7d9d43d133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5205677$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1818538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ooshima, H. (Osaka City University, Osaka, Japan)</creatorcontrib><creatorcontrib>Kurakake, M</creatorcontrib><creatorcontrib>Kato, J</creatorcontrib><creatorcontrib>Harano, Y</creatorcontrib><title>Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase from Trichoderma viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction, that is always observed in enzymatic hydrolysis of cellulose, could be explained by a decline of specific activity of adsorbed enzyme, and it was implied that the decline results from a loss of synergistic action between endoglucanase and exoglucanase. An empirical equation expresses the change of hydrolysis rate during the reaction and also shows that the change of the hydrolysis rate is caused by the decline of the specific enzymatic activity of adsorbed enzyme.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ADSORCION</subject><subject>ADSORPTION</subject><subject>avicel</subject><subject>BETA-GLUCANASE</subject><subject>BETA-GLUCOSIDASE</subject><subject>beta-Glucosidase - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CELLULASE</subject><subject>Cellulase - drug effects</subject><subject>Cellulase - metabolism</subject><subject>CELLULOSE</subject><subject>Cellulose - chemistry</subject><subject>Cellulose - metabolism</subject><subject>CELLULOSES</subject><subject>CELULASA</subject><subject>CELULOSA</subject><subject>endoglucanase</subject><subject>enzymatic activity</subject><subject>Enzyme engineering</subject><subject>enzyme inactivation</subject><subject>ENZYMIC ACTIVITY</subject><subject>exoglucanase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLICOSIDASAS</subject><subject>Glucan 1,3-beta-Glucosidase</subject><subject>Glucose - pharmacology</subject><subject>GLUCOSIDASA</subject><subject>GLUCOSIDASE</subject><subject>GLUCOSIDASES</subject><subject>GLYCOSIDASE</subject><subject>GLYCOSIDASES</subject><subject>HIDROLISIS</subject><subject>HYDROLYSE</subject><subject>HYDROLYSIS</subject><subject>INHIBICION</subject><subject>INHIBITION</subject><subject>Kinetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>Protein Denaturation</subject><subject>SINERGISMO</subject><subject>SYNERGISM</subject><subject>SYNERGISME</subject><subject>TRICHODERMA</subject><subject>Trichoderma - enzymology</subject><subject>TRICHODERMA VIRIDE</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M9LHTEQB_BQFPvUXnosFHIoHgprM8nm19GKrwqCB6vXZV6Sfabs22iyK2z_-u7jPerR0zAzH4bhS8hnYOfAmP7xc8m45aAl_0AWIKWt5h4OyIJxLSrOjf1Ijkv5wxhwI_UROQIDRgqzII9X_d9pg0N0FN0QX-Mw0dRSF7pu7LAEir6kvAqepn4_Tdtp72kcCnVP2K8D9WOO_Zo-TT6nbiqxnJLDFrsSPu3rCXlYXv2-vK5u737dXF7cVk4oGCoIrkUuV8EJp93KSiNQAzghWa3U9luNKJRRUnFZO3CeBSa99tbXwoMQJ-Rsd_c5p5cxlKHZxLJ9E_uQxtLoOYCaM_UuBMWVZaae4fcddDmVkkPbPOe4wTw1wJpt2s1b2jP-ur86rjbBv9FdvPP-236PxWHXZuxdLP-Z5EwqrWf2ZcdaTA2u80we7i1YMBbEP9BVji0</recordid><startdate>19911201</startdate><enddate>19911201</enddate><creator>Ooshima, H. (Osaka City University, Osaka, Japan)</creator><creator>Kurakake, M</creator><creator>Kato, J</creator><creator>Harano, Y</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19911201</creationdate><title>Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis</title><author>Ooshima, H. (Osaka City University, Osaka, Japan) ; Kurakake, M ; Kato, J ; Harano, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-1ecfa25bec3c7cb9583a711c35046601287aa368656254c1cd0e05d7d9d43d133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ADSORCION</topic><topic>ADSORPTION</topic><topic>avicel</topic><topic>BETA-GLUCANASE</topic><topic>BETA-GLUCOSIDASE</topic><topic>beta-Glucosidase - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CELLULASE</topic><topic>Cellulase - drug effects</topic><topic>Cellulase - metabolism</topic><topic>CELLULOSE</topic><topic>Cellulose - chemistry</topic><topic>Cellulose - metabolism</topic><topic>CELLULOSES</topic><topic>CELULASA</topic><topic>CELULOSA</topic><topic>endoglucanase</topic><topic>enzymatic activity</topic><topic>Enzyme engineering</topic><topic>enzyme inactivation</topic><topic>ENZYMIC ACTIVITY</topic><topic>exoglucanase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLICOSIDASAS</topic><topic>Glucan 1,3-beta-Glucosidase</topic><topic>Glucose - pharmacology</topic><topic>GLUCOSIDASA</topic><topic>GLUCOSIDASE</topic><topic>GLUCOSIDASES</topic><topic>GLYCOSIDASE</topic><topic>GLYCOSIDASES</topic><topic>HIDROLISIS</topic><topic>HYDROLYSE</topic><topic>HYDROLYSIS</topic><topic>INHIBICION</topic><topic>INHIBITION</topic><topic>Kinetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>Protein Denaturation</topic><topic>SINERGISMO</topic><topic>SYNERGISM</topic><topic>SYNERGISME</topic><topic>TRICHODERMA</topic><topic>Trichoderma - enzymology</topic><topic>TRICHODERMA VIRIDE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ooshima, H. (Osaka City University, Osaka, Japan)</creatorcontrib><creatorcontrib>Kurakake, M</creatorcontrib><creatorcontrib>Kato, J</creatorcontrib><creatorcontrib>Harano, Y</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ooshima, H. (Osaka City University, Osaka, Japan)</au><au>Kurakake, M</au><au>Kato, J</au><au>Harano, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>1991-12-01</date><risdate>1991</risdate><volume>31</volume><issue>3</issue><spage>253</spage><epage>266</epage><pages>253-266</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase from Trichoderma viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction, that is always observed in enzymatic hydrolysis of cellulose, could be explained by a decline of specific activity of adsorbed enzyme, and it was implied that the decline results from a loss of synergistic action between endoglucanase and exoglucanase. An empirical equation expresses the change of hydrolysis rate during the reaction and also shows that the change of the hydrolysis rate is caused by the decline of the specific enzymatic activity of adsorbed enzyme.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>1818538</pmid><doi>10.1007/BF02921752</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 1991-12, Vol.31 (3), p.253-266 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72734206 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADSORCION ADSORPTION avicel BETA-GLUCANASE BETA-GLUCOSIDASE beta-Glucosidase - metabolism Biological and medical sciences Biotechnology CELLULASE Cellulase - drug effects Cellulase - metabolism CELLULOSE Cellulose - chemistry Cellulose - metabolism CELLULOSES CELULASA CELULOSA endoglucanase enzymatic activity Enzyme engineering enzyme inactivation ENZYMIC ACTIVITY exoglucanase Fundamental and applied biological sciences. Psychology GLICOSIDASAS Glucan 1,3-beta-Glucosidase Glucose - pharmacology GLUCOSIDASA GLUCOSIDASE GLUCOSIDASES GLYCOSIDASE GLYCOSIDASES HIDROLISIS HYDROLYSE HYDROLYSIS INHIBICION INHIBITION Kinetics Methods. Procedures. Technologies Miscellaneous Protein Denaturation SINERGISMO SYNERGISM SYNERGISME TRICHODERMA Trichoderma - enzymology TRICHODERMA VIRIDE |
| title | Enzymatic activity of cellulase adsorbed on cellulose and its change during hydrolysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T01%3A51%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzymatic%20activity%20of%20cellulase%20adsorbed%20on%20cellulose%20and%20its%20change%20during%20hydrolysis&rft.jtitle=Applied%20biochemistry%20and%20biotechnology&rft.au=Ooshima,%20H.%20(Osaka%20City%20University,%20Osaka,%20Japan)&rft.date=1991-12-01&rft.volume=31&rft.issue=3&rft.spage=253&rft.epage=266&rft.pages=253-266&rft.issn=0273-2289&rft.eissn=1559-0291&rft.coden=ABIBDL&rft_id=info:doi/10.1007/BF02921752&rft_dat=%3Cproquest_cross%3E72734206%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16269084&rft_id=info:pmid/1818538&rfr_iscdi=true |