Ethanol-induced Cl − flux in rat cerebellar granule cells as measured by a fluorescent probe

Cl − fluxes through the GABA A receptor gated ion channels in cultured rat cerebellar granule cells were measured using the chloride-sensitive fluorescent probe SPQ (6-methoxy-N-(3-sulphopropyl)quinolinium) incorporated into the cells. The fluorescence of SPQ is quenched by Cl − ions. The cells were...

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Veröffentlicht in:	Brain research 1991-12, Vol.568 (1), p.55-60
Hauptverfasser:	Engblom, A.C., Holopainen, I., Åkerman, K.E.O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcoholism and acute alcohol poisoning Animals Biological and medical sciences Cells, Cultured Cerebellar granule cell Cerebellum - cytology Cerebellum - drug effects Cerebellum - metabolism Chloride flux Chlorides - metabolism Dose-Response Relationship, Drug Ethanol - pharmacology Fluorescent chloride probe Furosemide - pharmacology gamma-Aminobutyric Acid - pharmacology Ion Channel Gating - drug effects Ion Channels - drug effects Ion Channels - metabolism Kinetics Medical sciences Rats Rats, Inbred Strains Receptors, GABA-A - drug effects Receptors, GABA-A - physiology Toxicology γ-Aminobutyric acid receptor complex
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Zusammenfassung:	Cl − fluxes through the GABA A receptor gated ion channels in cultured rat cerebellar granule cells were measured using the chloride-sensitive fluorescent probe SPQ (6-methoxy-N-(3-sulphopropyl)quinolinium) incorporated into the cells. The fluorescence of SPQ is quenched by Cl − ions. The cells were bathed in a low Cl − medium so that the Cl − gradient was directed outward. Ethanol increased the SPQ fluorescence indicating a decrease in intracellular Cl − due to Cl − efflux. Picrotoxin inhibited the effect at low concentrations of ethanol (
ISSN:	0006-8993 1872-6240
DOI:	10.1016/0006-8993(91)91378-E