Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD65 and GAD67
: γ‐Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from each other in size, sequence, immunoreactiv...
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| Veröffentlicht in: | Journal of neurochemistry 1992-02, Vol.58 (2), p.618-627 |
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| creator | Tillakaratne, Niranjala J. K. Erlander, Mark G. Collard, Michael W. Greif, Karen F. Tobin, Allan J. |
| description | : γ‐Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from each other in size, sequence, immunoreactivity, and their interaction with the cofactor pyridoxal 5′‐phosphate (PLP). We used cDNAs and antibodies specific to GAD65 and GAD67 to study the molecular identity of GADs in peripheral tissues. We detected GAD and GAD mRNAs in rat oviduct and testis. In oviduct, the size of GAD, its response to PLP, its immunoreactivity, and its hybridization to specific RNA and DNA probes all indicate the specific expression of the GAD65 gene. In contrast, rat testis expresses the GAD67 gene. The GAD in these two reproductive tissues is not in neurons but in nonneural cells. The localization of brain GAD and GAD mRNAs in the mucosal epithelial cells of the oviduct and in spermatocytes and spermatids of the testis shows that GAD is not limited to neurons and that GABA may have functions other than neurotransmission. |
| doi_str_mv | 10.1111/j.1471-4159.1992.tb09763.x |
| format | Article |
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K. ; Erlander, Mark G. ; Collard, Michael W. ; Greif, Karen F. ; Tobin, Allan J.</creator><creatorcontrib>Tillakaratne, Niranjala J. K. ; Erlander, Mark G. ; Collard, Michael W. ; Greif, Karen F. ; Tobin, Allan J.</creatorcontrib><description>: γ‐Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from each other in size, sequence, immunoreactivity, and their interaction with the cofactor pyridoxal 5′‐phosphate (PLP). We used cDNAs and antibodies specific to GAD65 and GAD67 to study the molecular identity of GADs in peripheral tissues. We detected GAD and GAD mRNAs in rat oviduct and testis. In oviduct, the size of GAD, its response to PLP, its immunoreactivity, and its hybridization to specific RNA and DNA probes all indicate the specific expression of the GAD65 gene. In contrast, rat testis expresses the GAD67 gene. The GAD in these two reproductive tissues is not in neurons but in nonneural cells. The localization of brain GAD and GAD mRNAs in the mucosal epithelial cells of the oviduct and in spermatocytes and spermatids of the testis shows that GAD is not limited to neurons and that GABA may have functions other than neurotransmission.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1992.tb09763.x</identifier><identifier>PMID: 1729406</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Animals, Newborn ; Biological and medical sciences ; Enzymes and enzyme inhibitors ; Female ; Fundamental and applied biological sciences. Psychology ; Germ Cells - metabolism ; Glutamate decarboxylase ; Glutamate Decarboxylase - chemistry ; Glutamate Decarboxylase - genetics ; Glutamate Decarboxylase - metabolism ; Immunohistochemistry ; Lyases ; Male ; Molecular Weight ; mRNAs ; Mucous Membrane - enzymology ; Oviduct ; Oviducts - cytology ; Oviducts - enzymology ; Pyridoxal Phosphate - pharmacology ; Rats ; RNA, Messenger - metabolism ; Testis ; Testis - cytology ; Testis - enzymology ; Testis - growth & development ; γ‐Aminobutyric acid</subject><ispartof>Journal of neurochemistry, 1992-02, Vol.58 (2), p.618-627</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1992.tb09763.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1992.tb09763.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5109226$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1729406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tillakaratne, Niranjala J. K.</creatorcontrib><creatorcontrib>Erlander, Mark G.</creatorcontrib><creatorcontrib>Collard, Michael W.</creatorcontrib><creatorcontrib>Greif, Karen F.</creatorcontrib><creatorcontrib>Tobin, Allan J.</creatorcontrib><title>Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD65 and GAD67</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: γ‐Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from each other in size, sequence, immunoreactivity, and their interaction with the cofactor pyridoxal 5′‐phosphate (PLP). We used cDNAs and antibodies specific to GAD65 and GAD67 to study the molecular identity of GADs in peripheral tissues. We detected GAD and GAD mRNAs in rat oviduct and testis. In oviduct, the size of GAD, its response to PLP, its immunoreactivity, and its hybridization to specific RNA and DNA probes all indicate the specific expression of the GAD65 gene. In contrast, rat testis expresses the GAD67 gene. The GAD in these two reproductive tissues is not in neurons but in nonneural cells. The localization of brain GAD and GAD mRNAs in the mucosal epithelial cells of the oviduct and in spermatocytes and spermatids of the testis shows that GAD is not limited to neurons and that GABA may have functions other than neurotransmission.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Germ Cells - metabolism</subject><subject>Glutamate decarboxylase</subject><subject>Glutamate Decarboxylase - chemistry</subject><subject>Glutamate Decarboxylase - genetics</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Immunohistochemistry</subject><subject>Lyases</subject><subject>Male</subject><subject>Molecular Weight</subject><subject>mRNAs</subject><subject>Mucous Membrane - enzymology</subject><subject>Oviduct</subject><subject>Oviducts - cytology</subject><subject>Oviducts - enzymology</subject><subject>Pyridoxal Phosphate - pharmacology</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>Testis</subject><subject>Testis - cytology</subject><subject>Testis - enzymology</subject><subject>Testis - growth & development</subject><subject>γ‐Aminobutyric acid</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkVFr2zAQx0Xp6LK2H2EgRtmbvTtZtqw9FEKSZSshgZE9C1mWQMGxU8vukm8_ezWtXk7w_91x0o-QLwgxDufbIUYuMOKYyhilZHFXgBRZEp-vyOwtuiYzAMaiBDj7SD6FcADAjGd4Q25QMMkhm5HnddV3-qg7S5fW6LZozpdKBxuor-m2qWvbt7qiC1tVgTaO_tYd3dvQ-UB1XdLdiy97032nS--cbW3d-YFenU-tDcE39diyni-z9D893sQd-eB0Fez9VG_Jnx-r_eJntNmtfy3mm-jAIc8j5DkKw6QwUHLBXCGB5WkiEIuUpyidcRnHDBBFkcicGeu0BuBJKtIyNza5JV9f557a5rkfVlZHH8zwDl3bpg9KMMFyzmAAP09gXxxtqU6tP-r2oqY_GvKHKdfB6Mq1ujY-vGEpgmRsxB5fsb--spf3KaBGZ-qgRjFqFKNGZ2pyps7qabvIME_-AYO-iJg</recordid><startdate>199202</startdate><enddate>199202</enddate><creator>Tillakaratne, Niranjala J. K.</creator><creator>Erlander, Mark G.</creator><creator>Collard, Michael W.</creator><creator>Greif, Karen F.</creator><creator>Tobin, Allan J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199202</creationdate><title>Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD65 and GAD67</title><author>Tillakaratne, Niranjala J. K. ; Erlander, Mark G. ; Collard, Michael W. ; Greif, Karen F. ; Tobin, Allan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j4088-14817c297c0d472fb902853711b54519fcf64160117b3982cefaa0043575d8ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Germ Cells - metabolism</topic><topic>Glutamate decarboxylase</topic><topic>Glutamate Decarboxylase - chemistry</topic><topic>Glutamate Decarboxylase - genetics</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Immunohistochemistry</topic><topic>Lyases</topic><topic>Male</topic><topic>Molecular Weight</topic><topic>mRNAs</topic><topic>Mucous Membrane - enzymology</topic><topic>Oviduct</topic><topic>Oviducts - cytology</topic><topic>Oviducts - enzymology</topic><topic>Pyridoxal Phosphate - pharmacology</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>Testis</topic><topic>Testis - cytology</topic><topic>Testis - enzymology</topic><topic>Testis - growth & development</topic><topic>γ‐Aminobutyric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tillakaratne, Niranjala J. K.</creatorcontrib><creatorcontrib>Erlander, Mark G.</creatorcontrib><creatorcontrib>Collard, Michael W.</creatorcontrib><creatorcontrib>Greif, Karen F.</creatorcontrib><creatorcontrib>Tobin, Allan J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tillakaratne, Niranjala J. K.</au><au>Erlander, Mark G.</au><au>Collard, Michael W.</au><au>Greif, Karen F.</au><au>Tobin, Allan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD65 and GAD67</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1992-02</date><risdate>1992</risdate><volume>58</volume><issue>2</issue><spage>618</spage><epage>627</epage><pages>618-627</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: γ‐Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from each other in size, sequence, immunoreactivity, and their interaction with the cofactor pyridoxal 5′‐phosphate (PLP). We used cDNAs and antibodies specific to GAD65 and GAD67 to study the molecular identity of GADs in peripheral tissues. We detected GAD and GAD mRNAs in rat oviduct and testis. In oviduct, the size of GAD, its response to PLP, its immunoreactivity, and its hybridization to specific RNA and DNA probes all indicate the specific expression of the GAD65 gene. In contrast, rat testis expresses the GAD67 gene. The GAD in these two reproductive tissues is not in neurons but in nonneural cells. The localization of brain GAD and GAD mRNAs in the mucosal epithelial cells of the oviduct and in spermatocytes and spermatids of the testis shows that GAD is not limited to neurons and that GABA may have functions other than neurotransmission.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1729406</pmid><doi>10.1111/j.1471-4159.1992.tb09763.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Animals, Newborn Biological and medical sciences Enzymes and enzyme inhibitors Female Fundamental and applied biological sciences. Psychology Germ Cells - metabolism Glutamate decarboxylase Glutamate Decarboxylase - chemistry Glutamate Decarboxylase - genetics Glutamate Decarboxylase - metabolism Immunohistochemistry Lyases Male Molecular Weight mRNAs Mucous Membrane - enzymology Oviduct Oviducts - cytology Oviducts - enzymology Pyridoxal Phosphate - pharmacology Rats RNA, Messenger - metabolism Testis Testis - cytology Testis - enzymology Testis - growth & development γ‐Aminobutyric acid |
| title | Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD65 and GAD67 |
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