Neurochordins of higher vertebrates: Similarities in P-epitope-bearing polypeptide composition and a study of P-epitope expression in cultured neural tissue cells
Neurochordins are a family of high M r P-epitope-bearing neural tissue glycoproteins. Comparison of SDS-agarose electrophoretic patterns of extracts from human, rat, mouse and chicken brain after immunostaining of blots with an anti-P-epitope MAb At5 demonstrated that in each case neurochordins from...
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| Veröffentlicht in: | Neuroscience letters 1991-12, Vol.133 (2), p.284-286 |
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| container_title | Neuroscience letters |
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| creator | Preobrazhensky, A.A. Khalansky, A.S. Kozlova, M.V. |
| description | Neurochordins are a family of high
M
r P-epitope-bearing neural tissue glycoproteins. Comparison of SDS-agarose electrophoretic patterns of extracts from human, rat, mouse and chicken brain after immunostaining of blots with an anti-P-epitope MAb At5 demonstrated that in each case neurochordins from the A, B and C group were present. A similar result was obtained when polyclonal serum against total human neurochordin was used. The data favours the idea of high evolutional conservatism of neurochordins of higher vertebrate species. Expression of the P-epitope on glial cells in culture was studied. Strong immunostaining of oligodendrocytes with MAb At5 and the absence of this staining in astrocytes and Schwann cells make it possible to use At5 for identification of cell types in tissue culture experiments as well as for differential diagnostics of brain tumours. |
| doi_str_mv | 10.1016/0304-3940(91)90589-L |
| format | Article |
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r P-epitope-bearing neural tissue glycoproteins. Comparison of SDS-agarose electrophoretic patterns of extracts from human, rat, mouse and chicken brain after immunostaining of blots with an anti-P-epitope MAb At5 demonstrated that in each case neurochordins from the A, B and C group were present. A similar result was obtained when polyclonal serum against total human neurochordin was used. The data favours the idea of high evolutional conservatism of neurochordins of higher vertebrate species. Expression of the P-epitope on glial cells in culture was studied. Strong immunostaining of oligodendrocytes with MAb At5 and the absence of this staining in astrocytes and Schwann cells make it possible to use At5 for identification of cell types in tissue culture experiments as well as for differential diagnostics of brain tumours.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(91)90589-L</identifier><identifier>PMID: 1726187</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Antibodies, Monoclonal ; Biochemistry and metabolism ; Biological and medical sciences ; Biological Evolution ; Brain - cytology ; Brain Chemistry ; Cells, Cultured ; Central nervous system ; Chickens ; Electrophoresis, Agar Gel ; Epitopes - analysis ; Evolutionary conservatism ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; High Mr glycoprotein ; Humans ; Immunoblotting - methods ; Membrane Glycoproteins - analysis ; Membrane Glycoproteins - immunology ; Mice ; Molecular Weight ; Nerve Tissue Proteins - analysis ; Nerve Tissue Proteins - immunology ; Neural tissue specificity ; Neurochordin ; Neuroglia - cytology ; Oligodendrocyte ; Oligodendroglia - cytology ; Rats ; Species Specificity ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1991-12, Vol.133 (2), p.284-286</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-570f1ef73018e4498bde57c117a84fcb6bf479d331063aa5509f570b8a5ba9593</citedby><cites>FETCH-LOGICAL-c386t-570f1ef73018e4498bde57c117a84fcb6bf479d331063aa5509f570b8a5ba9593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/030439409190589L$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5013138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1726187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Preobrazhensky, A.A.</creatorcontrib><creatorcontrib>Khalansky, A.S.</creatorcontrib><creatorcontrib>Kozlova, M.V.</creatorcontrib><title>Neurochordins of higher vertebrates: Similarities in P-epitope-bearing polypeptide composition and a study of P-epitope expression in cultured neural tissue cells</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Neurochordins are a family of high
M
r P-epitope-bearing neural tissue glycoproteins. Comparison of SDS-agarose electrophoretic patterns of extracts from human, rat, mouse and chicken brain after immunostaining of blots with an anti-P-epitope MAb At5 demonstrated that in each case neurochordins from the A, B and C group were present. A similar result was obtained when polyclonal serum against total human neurochordin was used. The data favours the idea of high evolutional conservatism of neurochordins of higher vertebrate species. Expression of the P-epitope on glial cells in culture was studied. Strong immunostaining of oligodendrocytes with MAb At5 and the absence of this staining in astrocytes and Schwann cells make it possible to use At5 for identification of cell types in tissue culture experiments as well as for differential diagnostics of brain tumours.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Brain - cytology</subject><subject>Brain Chemistry</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>Chickens</subject><subject>Electrophoresis, Agar Gel</subject><subject>Epitopes - analysis</subject><subject>Evolutionary conservatism</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>High Mr glycoprotein</subject><subject>Humans</subject><subject>Immunoblotting - methods</subject><subject>Membrane Glycoproteins - analysis</subject><subject>Membrane Glycoproteins - immunology</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - immunology</subject><subject>Neural tissue specificity</subject><subject>Neurochordin</subject><subject>Neuroglia - cytology</subject><subject>Oligodendrocyte</subject><subject>Oligodendroglia - cytology</subject><subject>Rats</subject><subject>Species Specificity</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV-L1DAUxYMo67j6DRTyIKIP1aRpmsYHQRb_waCC-hzS5HYn0mm6uenifB0_qakzjG8-BXJ_53A4h5DHnL3kjLevmGBNJXTDnmv-QjPZ6Wp7h2x4p-pKaVXfJZszcp88QPzJGJNcNhfkgqu6LeCG_P4MS4puF5MPE9I40F243kGit5Ay9MlmwNf0W9iH0aaQAyANE_1awRxynKHqoXxP13SO42GGOQcP1MX9HLHAcaJ28tRSzIs_rOZnIYVfcwLElSmGbhnzksDTqcSxI80BcSlOMI74kNwb7Ijw6PRekh_v332_-lhtv3z4dPV2WznRtbmSig0cBiUY76BpdNd7kMpxrmzXDK5v-6FR2gvBWSuslZLpoWj6zsreaqnFJXl29J1TvFkAs9kHXBPYCeKCRtWqbkqZBWyOoEsRMcFg5hT2Nh0MZ2adxqy9m7V3o7n5O43ZFtmTk__S78H_Ex23KPenp7tFZ8ch2ckFPGOSccFFV7A3RwxKF7cBkkEXYHLgQwKXjY_h_zn-AD0_rlY</recordid><startdate>19911209</startdate><enddate>19911209</enddate><creator>Preobrazhensky, A.A.</creator><creator>Khalansky, A.S.</creator><creator>Kozlova, M.V.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911209</creationdate><title>Neurochordins of higher vertebrates: Similarities in P-epitope-bearing polypeptide composition and a study of P-epitope expression in cultured neural tissue cells</title><author>Preobrazhensky, A.A. ; Khalansky, A.S. ; Kozlova, M.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-570f1ef73018e4498bde57c117a84fcb6bf479d331063aa5509f570b8a5ba9593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Brain - cytology</topic><topic>Brain Chemistry</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>Chickens</topic><topic>Electrophoresis, Agar Gel</topic><topic>Epitopes - analysis</topic><topic>Evolutionary conservatism</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>High Mr glycoprotein</topic><topic>Humans</topic><topic>Immunoblotting - methods</topic><topic>Membrane Glycoproteins - analysis</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Nerve Tissue Proteins - immunology</topic><topic>Neural tissue specificity</topic><topic>Neurochordin</topic><topic>Neuroglia - cytology</topic><topic>Oligodendrocyte</topic><topic>Oligodendroglia - cytology</topic><topic>Rats</topic><topic>Species Specificity</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Preobrazhensky, A.A.</creatorcontrib><creatorcontrib>Khalansky, A.S.</creatorcontrib><creatorcontrib>Kozlova, M.V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Preobrazhensky, A.A.</au><au>Khalansky, A.S.</au><au>Kozlova, M.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neurochordins of higher vertebrates: Similarities in P-epitope-bearing polypeptide composition and a study of P-epitope expression in cultured neural tissue cells</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1991-12-09</date><risdate>1991</risdate><volume>133</volume><issue>2</issue><spage>284</spage><epage>286</epage><pages>284-286</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Neurochordins are a family of high
M
r P-epitope-bearing neural tissue glycoproteins. Comparison of SDS-agarose electrophoretic patterns of extracts from human, rat, mouse and chicken brain after immunostaining of blots with an anti-P-epitope MAb At5 demonstrated that in each case neurochordins from the A, B and C group were present. A similar result was obtained when polyclonal serum against total human neurochordin was used. The data favours the idea of high evolutional conservatism of neurochordins of higher vertebrate species. Expression of the P-epitope on glial cells in culture was studied. Strong immunostaining of oligodendrocytes with MAb At5 and the absence of this staining in astrocytes and Schwann cells make it possible to use At5 for identification of cell types in tissue culture experiments as well as for differential diagnostics of brain tumours.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>1726187</pmid><doi>10.1016/0304-3940(91)90589-L</doi><tpages>3</tpages></addata></record> |
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| ispartof | Neuroscience letters, 1991-12, Vol.133 (2), p.284-286 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal Biochemistry and metabolism Biological and medical sciences Biological Evolution Brain - cytology Brain Chemistry Cells, Cultured Central nervous system Chickens Electrophoresis, Agar Gel Epitopes - analysis Evolutionary conservatism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology High Mr glycoprotein Humans Immunoblotting - methods Membrane Glycoproteins - analysis Membrane Glycoproteins - immunology Mice Molecular Weight Nerve Tissue Proteins - analysis Nerve Tissue Proteins - immunology Neural tissue specificity Neurochordin Neuroglia - cytology Oligodendrocyte Oligodendroglia - cytology Rats Species Specificity Vertebrates: nervous system and sense organs |
| title | Neurochordins of higher vertebrates: Similarities in P-epitope-bearing polypeptide composition and a study of P-epitope expression in cultured neural tissue cells |
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