DNA polymerase α in the fission yeast Schizosaccharomyces pombe: Identification and tracing of the catalytic subunit during the cell cycle

A recombinant protein was obtained in Escherichia coli by subcloning part of the Schizosaccharomyces pombe POL1 gene at the 3′-end of lacZ. Antibodies raised against this protein were used to identify the POL1 gene product in extracts of exponentially growing S. pombe cells. A major 170-kDa protein,...

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