Multidimensional chromatography coupled to electrospray ionization time-of-flight mass spectrometry as an alternative to two-dimensional gels for the identification and analysis of complex mixtures of intact proteins

The limitations of 2-D gels for global proteomics have encouraged the development of alternative approaches for identifying proteins in complicated mixtures, and determining their modification state. In this work, we describe the application of multidimensional liquid chromatography (SCX-RPLC) coupl...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2002-12, Vol.782 (1), p.267-289
Hauptverfasser:	Liu, Hongji, Berger, Scott J, Chakraborty, Asish B, Plumb, Robert S, Cohen, Steven A
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Ion Exchange Electrophoresis, Gel, Two-Dimensional - methods Proteins Ribosomal Proteins - analysis Ribosomal Proteins - chemistry Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
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