KaiA-Stimulated KaiC Phosphorylation in Circadian Timing Loops in Cyanobacteria
Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2002-11, Vol.99 (24), p.15788-15793 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 15793 |
|---|---|
| container_issue | 24 |
| container_start_page | 15788 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 99 |
| creator | Iwasaki, Hideo Nishiwaki, Taeko Kitayama, Yohko Nakajima, Masato Kondo, Takao |
| description | Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC phosphorylation and activated in vitro KaiC autophosphorylation. These effects of KaiA were attenuated by the kaiA2 long period mutation. Both the long period phenotype and the abnormal KaiC phosphorylation in this mutant were suppressed by a previously undocumented kaiC mutation. We propose that KaiA-stimulated circadian KaiC phosphorylation is important for circadian timing. |
| doi_str_mv | 10.1073/pnas.222467299 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_72718482</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3073850</jstor_id><sourcerecordid>3073850</sourcerecordid><originalsourceid>FETCH-LOGICAL-c587t-3c51e20305a9ebe2043f5783f66a84bf3f8907184940ceaaaa6c81143e8607a03</originalsourceid><addsrcrecordid>eNqFkUtvEzEUhS0EomlhywrBiAViM-H6MWN7waKKeFSNVCTK2nIcT-Noxp7aM4j8-3qaEKAL8MaP8x37-h6EXmCYY-D0fe91mhNCWM2JlI_QDIPEZc0kPEYzAMJLwQg7QacpbQFAVgKeohNMqMQUYIauLrU7L78NrhtbPdh1kfeL4usmpH4T4i6fueAL54uFi0avnfbFteucvymWIfTpXtlpH1baDDY6_Qw9aXSb7PPDfIa-f_p4vfhSLq8-XyzOl6WpBB9KaipsCVCotLSrvGK0qbigTV1rwVYNbYQEjgWTDIzVedRGYMyoFTVwDfQMfdjf24-rzq6N9UPUreqj63TcqaCd-lvxbqNuwg-FKeeSZf_bgz-G29GmQXUuGdu22tswJsXJ9Log_wWxqDHUgmfwzQNwG8bocxMUAcxyFGQqe76HTAwpRdscK8agpkDVFKg6BpoNr_7852_8kGAGXh-AyfhLllIRpnBuqcjEu38TqhnbdrA_h4y-3KPbNIR4ZGkuTFRA7wB1cr1E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201402720</pqid></control><display><type>article</type><title>KaiA-Stimulated KaiC Phosphorylation in Circadian Timing Loops in Cyanobacteria</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Iwasaki, Hideo ; Nishiwaki, Taeko ; Kitayama, Yohko ; Nakajima, Masato ; Kondo, Takao</creator><creatorcontrib>Iwasaki, Hideo ; Nishiwaki, Taeko ; Kitayama, Yohko ; Nakajima, Masato ; Kondo, Takao</creatorcontrib><description>Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC phosphorylation and activated in vitro KaiC autophosphorylation. These effects of KaiA were attenuated by the kaiA2 long period mutation. Both the long period phenotype and the abnormal KaiC phosphorylation in this mutant were suppressed by a previously undocumented kaiC mutation. We propose that KaiA-stimulated circadian KaiC phosphorylation is important for circadian timing.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.222467299</identifier><identifier>PMID: 12391300</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Alleles ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Biological Clocks - physiology ; Biological Sciences ; Bioluminescence ; Botany ; Circadian rhythm ; Circadian Rhythm - physiology ; Circadian Rhythm Signaling Peptides and Proteins ; clock protein ; Cyanobacteria - genetics ; Cyanobacteria - physiology ; Cyanobacteria - radiation effects ; Cyanophyta ; Feedback ; Gels ; Gene Expression Regulation, Bacterial ; Genes ; Genes, Reporter ; Genetic mutation ; Genetic screening ; Journalism ; KaiA protein ; KaiC protein ; Luminescent Measurements ; Mutation ; Negative feedback ; Phosphorylation ; Photoperiod ; Positive feedback ; Protein Processing, Post-Translational ; Suppression, Genetic ; Western blotting</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2002-11, Vol.99 (24), p.15788-15793</ispartof><rights>Copyright 1993-2002 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Nov 26, 2002</rights><rights>Copyright © 2002, The National Academy of Sciences</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-3c51e20305a9ebe2043f5783f66a84bf3f8907184940ceaaaa6c81143e8607a03</citedby><cites>FETCH-LOGICAL-c587t-3c51e20305a9ebe2043f5783f66a84bf3f8907184940ceaaaa6c81143e8607a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/99/24.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3073850$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3073850$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12391300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwasaki, Hideo</creatorcontrib><creatorcontrib>Nishiwaki, Taeko</creatorcontrib><creatorcontrib>Kitayama, Yohko</creatorcontrib><creatorcontrib>Nakajima, Masato</creatorcontrib><creatorcontrib>Kondo, Takao</creatorcontrib><title>KaiA-Stimulated KaiC Phosphorylation in Circadian Timing Loops in Cyanobacteria</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC phosphorylation and activated in vitro KaiC autophosphorylation. These effects of KaiA were attenuated by the kaiA2 long period mutation. Both the long period phenotype and the abnormal KaiC phosphorylation in this mutant were suppressed by a previously undocumented kaiC mutation. We propose that KaiA-stimulated circadian KaiC phosphorylation is important for circadian timing.</description><subject>Alleles</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Biological Clocks - physiology</subject><subject>Biological Sciences</subject><subject>Bioluminescence</subject><subject>Botany</subject><subject>Circadian rhythm</subject><subject>Circadian Rhythm - physiology</subject><subject>Circadian Rhythm Signaling Peptides and Proteins</subject><subject>clock protein</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - physiology</subject><subject>Cyanobacteria - radiation effects</subject><subject>Cyanophyta</subject><subject>Feedback</subject><subject>Gels</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genes, Reporter</subject><subject>Genetic mutation</subject><subject>Genetic screening</subject><subject>Journalism</subject><subject>KaiA protein</subject><subject>KaiC protein</subject><subject>Luminescent Measurements</subject><subject>Mutation</subject><subject>Negative feedback</subject><subject>Phosphorylation</subject><subject>Photoperiod</subject><subject>Positive feedback</subject><subject>Protein Processing, Post-Translational</subject><subject>Suppression, Genetic</subject><subject>Western blotting</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS0EomlhywrBiAViM-H6MWN7waKKeFSNVCTK2nIcT-Noxp7aM4j8-3qaEKAL8MaP8x37-h6EXmCYY-D0fe91mhNCWM2JlI_QDIPEZc0kPEYzAMJLwQg7QacpbQFAVgKeohNMqMQUYIauLrU7L78NrhtbPdh1kfeL4usmpH4T4i6fueAL54uFi0avnfbFteucvymWIfTpXtlpH1baDDY6_Qw9aXSb7PPDfIa-f_p4vfhSLq8-XyzOl6WpBB9KaipsCVCotLSrvGK0qbigTV1rwVYNbYQEjgWTDIzVedRGYMyoFTVwDfQMfdjf24-rzq6N9UPUreqj63TcqaCd-lvxbqNuwg-FKeeSZf_bgz-G29GmQXUuGdu22tswJsXJ9Log_wWxqDHUgmfwzQNwG8bocxMUAcxyFGQqe76HTAwpRdscK8agpkDVFKg6BpoNr_7852_8kGAGXh-AyfhLllIRpnBuqcjEu38TqhnbdrA_h4y-3KPbNIR4ZGkuTFRA7wB1cr1E</recordid><startdate>20021126</startdate><enddate>20021126</enddate><creator>Iwasaki, Hideo</creator><creator>Nishiwaki, Taeko</creator><creator>Kitayama, Yohko</creator><creator>Nakajima, Masato</creator><creator>Kondo, Takao</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20021126</creationdate><title>KaiA-Stimulated KaiC Phosphorylation in Circadian Timing Loops in Cyanobacteria</title><author>Iwasaki, Hideo ; Nishiwaki, Taeko ; Kitayama, Yohko ; Nakajima, Masato ; Kondo, Takao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-3c51e20305a9ebe2043f5783f66a84bf3f8907184940ceaaaa6c81143e8607a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Alleles</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Biological Clocks - physiology</topic><topic>Biological Sciences</topic><topic>Bioluminescence</topic><topic>Botany</topic><topic>Circadian rhythm</topic><topic>Circadian Rhythm - physiology</topic><topic>Circadian Rhythm Signaling Peptides and Proteins</topic><topic>clock protein</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - physiology</topic><topic>Cyanobacteria - radiation effects</topic><topic>Cyanophyta</topic><topic>Feedback</topic><topic>Gels</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genes, Reporter</topic><topic>Genetic mutation</topic><topic>Genetic screening</topic><topic>Journalism</topic><topic>KaiA protein</topic><topic>KaiC protein</topic><topic>Luminescent Measurements</topic><topic>Mutation</topic><topic>Negative feedback</topic><topic>Phosphorylation</topic><topic>Photoperiod</topic><topic>Positive feedback</topic><topic>Protein Processing, Post-Translational</topic><topic>Suppression, Genetic</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwasaki, Hideo</creatorcontrib><creatorcontrib>Nishiwaki, Taeko</creatorcontrib><creatorcontrib>Kitayama, Yohko</creatorcontrib><creatorcontrib>Nakajima, Masato</creatorcontrib><creatorcontrib>Kondo, Takao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwasaki, Hideo</au><au>Nishiwaki, Taeko</au><au>Kitayama, Yohko</au><au>Nakajima, Masato</au><au>Kondo, Takao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>KaiA-Stimulated KaiC Phosphorylation in Circadian Timing Loops in Cyanobacteria</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2002-11-26</date><risdate>2002</risdate><volume>99</volume><issue>24</issue><spage>15788</spage><epage>15793</epage><pages>15788-15793</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC phosphorylation and activated in vitro KaiC autophosphorylation. These effects of KaiA were attenuated by the kaiA2 long period mutation. Both the long period phenotype and the abnormal KaiC phosphorylation in this mutant were suppressed by a previously undocumented kaiC mutation. We propose that KaiA-stimulated circadian KaiC phosphorylation is important for circadian timing.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>12391300</pmid><doi>10.1073/pnas.222467299</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2002-11, Vol.99 (24), p.15788-15793 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72718482 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Alleles Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Proteins - physiology Biological Clocks - physiology Biological Sciences Bioluminescence Botany Circadian rhythm Circadian Rhythm - physiology Circadian Rhythm Signaling Peptides and Proteins clock protein Cyanobacteria - genetics Cyanobacteria - physiology Cyanobacteria - radiation effects Cyanophyta Feedback Gels Gene Expression Regulation, Bacterial Genes Genes, Reporter Genetic mutation Genetic screening Journalism KaiA protein KaiC protein Luminescent Measurements Mutation Negative feedback Phosphorylation Photoperiod Positive feedback Protein Processing, Post-Translational Suppression, Genetic Western blotting |
| title | KaiA-Stimulated KaiC Phosphorylation in Circadian Timing Loops in Cyanobacteria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T04%3A55%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=KaiA-Stimulated%20KaiC%20Phosphorylation%20in%20Circadian%20Timing%20Loops%20in%20Cyanobacteria&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Iwasaki,%20Hideo&rft.date=2002-11-26&rft.volume=99&rft.issue=24&rft.spage=15788&rft.epage=15793&rft.pages=15788-15793&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.222467299&rft_dat=%3Cjstor_proqu%3E3073850%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201402720&rft_id=info:pmid/12391300&rft_jstor_id=3073850&rfr_iscdi=true |