Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom
The present report describes the identification and molecular characterization of LiD1, a protein expressed in the venom gland of the brown spider Loxosceles intermedia. LiD1 belongs to a family of proteins with dermonecrotic activity and members of this family have been found in spiders from the ge...
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| Veröffentlicht in: | Toxicon (Oxford) 2002-12, Vol.40 (12), p.1691-1699 |
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| creator | Kalapothakis, Evanguedes Araujo, Simone Costa de Castro, Cibele Soares Mendes, Thais Melo Gomez, Marcus Vinı́cius Mangili, Oldemir C Gubert, Ida C Chávez-Olórtegui, Carlos |
| description | The present report describes the identification and molecular characterization of LiD1, a protein expressed in the venom gland of the brown spider
Loxosceles intermedia. LiD1 belongs to a family of proteins with dermonecrotic activity and members of this family have been found in spiders from the genus
Loxosceles. The necrotic lesions caused by this group of proteins may lead to serious socio-economic problems such as surgical tissue reconstitution and even patient death. LiD1 was cloned using a cDNA library constructed from the venom gland of
L. intermedia and antibodies against proteins with dermonecrotic activity isolated from the crude venom of this spider. The amino acid sequence deduced from the cDNA revealed a mature protein of approximately 31
kDa, with a pI of 7.37. The cDNA also revealed the existence of a signal peptide, a propeptide and also an untranslated 3′ region with 218 nucleotides. LiD1 was expressed as a protein fused with β-galactoside protein using the vector pBK-CMV, resulting in the recombinant protein recLiD1 with important immunological properties. recLiD1 was strongly recognised by anti-dermonecrotic antibodies and was also able to generate reactive antibodies against native dermonecrotic proteins isolated from the venom of
L. intermedia. |
| doi_str_mv | 10.1016/S0041-0101(02)00201-5 |
| format | Article |
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Loxosceles intermedia. LiD1 belongs to a family of proteins with dermonecrotic activity and members of this family have been found in spiders from the genus
Loxosceles. The necrotic lesions caused by this group of proteins may lead to serious socio-economic problems such as surgical tissue reconstitution and even patient death. LiD1 was cloned using a cDNA library constructed from the venom gland of
L. intermedia and antibodies against proteins with dermonecrotic activity isolated from the crude venom of this spider. The amino acid sequence deduced from the cDNA revealed a mature protein of approximately 31
kDa, with a pI of 7.37. The cDNA also revealed the existence of a signal peptide, a propeptide and also an untranslated 3′ region with 218 nucleotides. LiD1 was expressed as a protein fused with β-galactoside protein using the vector pBK-CMV, resulting in the recombinant protein recLiD1 with important immunological properties. recLiD1 was strongly recognised by anti-dermonecrotic antibodies and was also able to generate reactive antibodies against native dermonecrotic proteins isolated from the venom of
L. intermedia.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/S0041-0101(02)00201-5</identifier><identifier>PMID: 12457881</identifier><identifier>CODEN: TOXIA6</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animal poisons toxicology. Antivenoms ; Animals ; Base Sequence ; Biological and medical sciences ; cDNA ; Chemical Fractionation ; Cloning, Molecular ; Dermonecrotic toxin ; DNA, Complementary - genetics ; Expression ; Gene Expression ; Gene Library ; LiD1 ; Loxoceles intermedia ; Medical sciences ; Molecular Sequence Data ; Phosphoric Diester Hydrolases - genetics ; Phosphoric Diester Hydrolases - immunology ; Phosphoric Diester Hydrolases - metabolism ; Recombinant Proteins - genetics ; Spider Venoms - genetics ; Spider Venoms - immunology ; Spider Venoms - metabolism ; Spiders - physiology ; Toxicology</subject><ispartof>Toxicon (Oxford), 2002-12, Vol.40 (12), p.1691-1699</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-34f76e68a3a5c462037729a92ec9442f070932f731fd472ac415dfbd3a16f6283</citedby><cites>FETCH-LOGICAL-c422t-34f76e68a3a5c462037729a92ec9442f070932f731fd472ac415dfbd3a16f6283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0041010102002015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14043585$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12457881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalapothakis, Evanguedes</creatorcontrib><creatorcontrib>Araujo, Simone Costa</creatorcontrib><creatorcontrib>de Castro, Cibele Soares</creatorcontrib><creatorcontrib>Mendes, Thais Melo</creatorcontrib><creatorcontrib>Gomez, Marcus Vinı́cius</creatorcontrib><creatorcontrib>Mangili, Oldemir C</creatorcontrib><creatorcontrib>Gubert, Ida C</creatorcontrib><creatorcontrib>Chávez-Olórtegui, Carlos</creatorcontrib><title>Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>The present report describes the identification and molecular characterization of LiD1, a protein expressed in the venom gland of the brown spider
Loxosceles intermedia. LiD1 belongs to a family of proteins with dermonecrotic activity and members of this family have been found in spiders from the genus
Loxosceles. The necrotic lesions caused by this group of proteins may lead to serious socio-economic problems such as surgical tissue reconstitution and even patient death. LiD1 was cloned using a cDNA library constructed from the venom gland of
L. intermedia and antibodies against proteins with dermonecrotic activity isolated from the crude venom of this spider. The amino acid sequence deduced from the cDNA revealed a mature protein of approximately 31
kDa, with a pI of 7.37. The cDNA also revealed the existence of a signal peptide, a propeptide and also an untranslated 3′ region with 218 nucleotides. LiD1 was expressed as a protein fused with β-galactoside protein using the vector pBK-CMV, resulting in the recombinant protein recLiD1 with important immunological properties. recLiD1 was strongly recognised by anti-dermonecrotic antibodies and was also able to generate reactive antibodies against native dermonecrotic proteins isolated from the venom of
L. intermedia.</description><subject>Amino Acid Sequence</subject><subject>Animal poisons toxicology. Antivenoms</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA</subject><subject>Chemical Fractionation</subject><subject>Cloning, Molecular</subject><subject>Dermonecrotic toxin</subject><subject>DNA, Complementary - genetics</subject><subject>Expression</subject><subject>Gene Expression</subject><subject>Gene Library</subject><subject>LiD1</subject><subject>Loxoceles intermedia</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Phosphoric Diester Hydrolases - genetics</subject><subject>Phosphoric Diester Hydrolases - immunology</subject><subject>Phosphoric Diester Hydrolases - metabolism</subject><subject>Recombinant Proteins - genetics</subject><subject>Spider Venoms - genetics</subject><subject>Spider Venoms - immunology</subject><subject>Spider Venoms - metabolism</subject><subject>Spiders - physiology</subject><subject>Toxicology</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1ERYfCI4C8AYHUwPVP4mSFUPlppUEsgLXlOtfFKLGDnVTtY_SNcWZGdNmVravv-B6fQ8gLBu8YsOb9DwDJKij3N8DfAnBgVf2IbFirukqwGh6TzX_kmDzN-Q8AiLZrnpBjxmWt2pZtyN23OKBdBpOoHWLw4eqU4s2UMGcfAzWhp34clxCHeOWtGeiU4oRp9phpdHTrP7FTatbpjD5Ql-JI599Ie0xjDGjL3FvqzOiH250g3sRscShyH-YCYe8NzZMvAnqNIY7PyJEzQ8bnh_OE_Pry-efZebX9_vXi7OO2spLzuRLSqQab1ghTW9lwEErxznQcbScld6CgE9wpwVwvFTdWsrp3l70wrHENb8UJeb1_t3j_u2Ce9ehXZ4MJGJesFVesFW39IMjappZKyQLWe7D8OueETk_JjybdagZ6LU3vStNrIxq43pWm1wUvDwuWy5LHverQUgFeHQCTSwcumWB9vuckSFHvnH7Yc1hyu_aYdLYegy0ZJ7Sz7qN_wMo_Bqq06A</recordid><startdate>20021201</startdate><enddate>20021201</enddate><creator>Kalapothakis, Evanguedes</creator><creator>Araujo, Simone Costa</creator><creator>de Castro, Cibele Soares</creator><creator>Mendes, Thais Melo</creator><creator>Gomez, Marcus Vinı́cius</creator><creator>Mangili, Oldemir C</creator><creator>Gubert, Ida C</creator><creator>Chávez-Olórtegui, Carlos</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20021201</creationdate><title>Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom</title><author>Kalapothakis, Evanguedes ; Araujo, Simone Costa ; de Castro, Cibele Soares ; Mendes, Thais Melo ; Gomez, Marcus Vinı́cius ; Mangili, Oldemir C ; Gubert, Ida C ; Chávez-Olórtegui, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-34f76e68a3a5c462037729a92ec9442f070932f731fd472ac415dfbd3a16f6283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animal poisons toxicology. Antivenoms</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA</topic><topic>Chemical Fractionation</topic><topic>Cloning, Molecular</topic><topic>Dermonecrotic toxin</topic><topic>DNA, Complementary - genetics</topic><topic>Expression</topic><topic>Gene Expression</topic><topic>Gene Library</topic><topic>LiD1</topic><topic>Loxoceles intermedia</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Phosphoric Diester Hydrolases - genetics</topic><topic>Phosphoric Diester Hydrolases - immunology</topic><topic>Phosphoric Diester Hydrolases - metabolism</topic><topic>Recombinant Proteins - genetics</topic><topic>Spider Venoms - genetics</topic><topic>Spider Venoms - immunology</topic><topic>Spider Venoms - metabolism</topic><topic>Spiders - physiology</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kalapothakis, Evanguedes</creatorcontrib><creatorcontrib>Araujo, Simone Costa</creatorcontrib><creatorcontrib>de Castro, Cibele Soares</creatorcontrib><creatorcontrib>Mendes, Thais Melo</creatorcontrib><creatorcontrib>Gomez, Marcus Vinı́cius</creatorcontrib><creatorcontrib>Mangili, Oldemir C</creatorcontrib><creatorcontrib>Gubert, Ida C</creatorcontrib><creatorcontrib>Chávez-Olórtegui, Carlos</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalapothakis, Evanguedes</au><au>Araujo, Simone Costa</au><au>de Castro, Cibele Soares</au><au>Mendes, Thais Melo</au><au>Gomez, Marcus Vinı́cius</au><au>Mangili, Oldemir C</au><au>Gubert, Ida C</au><au>Chávez-Olórtegui, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>2002-12-01</date><risdate>2002</risdate><volume>40</volume><issue>12</issue><spage>1691</spage><epage>1699</epage><pages>1691-1699</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>The present report describes the identification and molecular characterization of LiD1, a protein expressed in the venom gland of the brown spider
Loxosceles intermedia. LiD1 belongs to a family of proteins with dermonecrotic activity and members of this family have been found in spiders from the genus
Loxosceles. The necrotic lesions caused by this group of proteins may lead to serious socio-economic problems such as surgical tissue reconstitution and even patient death. LiD1 was cloned using a cDNA library constructed from the venom gland of
L. intermedia and antibodies against proteins with dermonecrotic activity isolated from the crude venom of this spider. The amino acid sequence deduced from the cDNA revealed a mature protein of approximately 31
kDa, with a pI of 7.37. The cDNA also revealed the existence of a signal peptide, a propeptide and also an untranslated 3′ region with 218 nucleotides. LiD1 was expressed as a protein fused with β-galactoside protein using the vector pBK-CMV, resulting in the recombinant protein recLiD1 with important immunological properties. recLiD1 was strongly recognised by anti-dermonecrotic antibodies and was also able to generate reactive antibodies against native dermonecrotic proteins isolated from the venom of
L. intermedia.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12457881</pmid><doi>10.1016/S0041-0101(02)00201-5</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animal poisons toxicology. Antivenoms Animals Base Sequence Biological and medical sciences cDNA Chemical Fractionation Cloning, Molecular Dermonecrotic toxin DNA, Complementary - genetics Expression Gene Expression Gene Library LiD1 Loxoceles intermedia Medical sciences Molecular Sequence Data Phosphoric Diester Hydrolases - genetics Phosphoric Diester Hydrolases - immunology Phosphoric Diester Hydrolases - metabolism Recombinant Proteins - genetics Spider Venoms - genetics Spider Venoms - immunology Spider Venoms - metabolism Spiders - physiology Toxicology |
| title | Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom |
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