Development and mapping of microsatellite markers derived from chicken chromosome-specific libraries
Chromosome-specific painting probes and libraries were developed for chicken Macrochromosomes 1, 2, 3, and 4 by chromosome microisolation and microcloning. Fluorescent in situ hybridization results using the painting probes on normal chicken metaphase chromosomes indicated the purity and specificity...
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| Veröffentlicht in: | Poultry science 2002-11, Vol.81 (11), p.1644-1646 |
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| creator | Ambady, S Cheng, H H Ponce de León, F A |
| description | Chromosome-specific painting probes and libraries were developed for chicken Macrochromosomes 1, 2, 3, and 4 by chromosome microisolation and microcloning. Fluorescent in situ hybridization results using the painting probes on normal chicken metaphase chromosomes indicated the purity and specificity of each probe. Chromosome-specific libraries for chicken Macrochromosomes 1, 2, 3, and 4 were prepared in a phage vector. Fifty-two additional unique microsatellite markers of the (AC)n type were developed from these chromosome-specific libraries. These markers were mapped on the East Lansing reference population to increase the marker density on the four macrochromosomes. Results of the current study suggest that development of markers from chromosome-specific libraries is very useful for constructing high-density linkage maps for chicken macrochromosomes. |
| doi_str_mv | 10.1093/ps/81.11.1644 |
| format | Article |
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Fluorescent in situ hybridization results using the painting probes on normal chicken metaphase chromosomes indicated the purity and specificity of each probe. Chromosome-specific libraries for chicken Macrochromosomes 1, 2, 3, and 4 were prepared in a phage vector. Fifty-two additional unique microsatellite markers of the (AC)n type were developed from these chromosome-specific libraries. These markers were mapped on the East Lansing reference population to increase the marker density on the four macrochromosomes. Results of the current study suggest that development of markers from chromosome-specific libraries is very useful for constructing high-density linkage maps for chicken macrochromosomes.</description><identifier>ISSN: 0032-5791</identifier><identifier>DOI: 10.1093/ps/81.11.1644</identifier><identifier>PMID: 12455590</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Chickens - genetics ; Chromosome Mapping - veterinary ; Cloning, Molecular ; DNA Primers - chemistry ; DNA Probes - chemistry ; Gene Library ; Genetic Linkage ; In Situ Hybridization, Fluorescence - veterinary ; Metaphase - genetics ; Microsatellite Repeats</subject><ispartof>Poultry science, 2002-11, Vol.81 (11), p.1644-1646</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c328t-6f226d0a0bfd0ba525c731a79bc88ca8a50dab9b0d1cd6395f3ba8e3ba98d8533</citedby><cites>FETCH-LOGICAL-c328t-6f226d0a0bfd0ba525c731a79bc88ca8a50dab9b0d1cd6395f3ba8e3ba98d8533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12455590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ambady, S</creatorcontrib><creatorcontrib>Cheng, H H</creatorcontrib><creatorcontrib>Ponce de León, F A</creatorcontrib><title>Development and mapping of microsatellite markers derived from chicken chromosome-specific libraries</title><title>Poultry science</title><addtitle>Poult Sci</addtitle><description>Chromosome-specific painting probes and libraries were developed for chicken Macrochromosomes 1, 2, 3, and 4 by chromosome microisolation and microcloning. Fluorescent in situ hybridization results using the painting probes on normal chicken metaphase chromosomes indicated the purity and specificity of each probe. Chromosome-specific libraries for chicken Macrochromosomes 1, 2, 3, and 4 were prepared in a phage vector. Fifty-two additional unique microsatellite markers of the (AC)n type were developed from these chromosome-specific libraries. These markers were mapped on the East Lansing reference population to increase the marker density on the four macrochromosomes. Results of the current study suggest that development of markers from chromosome-specific libraries is very useful for constructing high-density linkage maps for chicken macrochromosomes.</description><subject>Animals</subject><subject>Chickens - genetics</subject><subject>Chromosome Mapping - veterinary</subject><subject>Cloning, Molecular</subject><subject>DNA Primers - chemistry</subject><subject>DNA Probes - chemistry</subject><subject>Gene Library</subject><subject>Genetic Linkage</subject><subject>In Situ Hybridization, Fluorescence - veterinary</subject><subject>Metaphase - genetics</subject><subject>Microsatellite Repeats</subject><issn>0032-5791</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUE1LAzEUzEGx9ePoVXLytm0-mk32KPUTCl70vOTjRWN3N2uyFfz3prQgDG94vGF4MwhdU7KgpOHLMS8VXdCCerU6QXNCOKuEbOgMnef8RQijdS3P0IyylRCiIXPk7uEHujj2MExYDw73ehzD8IGjx32wKWY9QdeFCcolbSFl7CCFH3DYp9hj-xnsFobCZYs59lDlEWzwweIumKRTgHyJTr3uMlwd-QK9Pz68rZ-rzevTy_puU1nO1FTVnrHaEU2Md8RowYSVnGrZGKuU1UoL4rRpDHHUupo3wnOjFZTRKKcE5xfo9uA7pvi9gzy1fci2vK8HiLvcSiapFFIWYXUQ7gPmBL4dUyj5fltK2n2V7ZhbRVtaUKos-puj8c704P7Vxx75H_HFdDQ</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Ambady, S</creator><creator>Cheng, H H</creator><creator>Ponce de León, F A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Development and mapping of microsatellite markers derived from chicken chromosome-specific libraries</title><author>Ambady, S ; Cheng, H H ; Ponce de León, F A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-6f226d0a0bfd0ba525c731a79bc88ca8a50dab9b0d1cd6395f3ba8e3ba98d8533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Chickens - genetics</topic><topic>Chromosome Mapping - veterinary</topic><topic>Cloning, Molecular</topic><topic>DNA Primers - chemistry</topic><topic>DNA Probes - chemistry</topic><topic>Gene Library</topic><topic>Genetic Linkage</topic><topic>In Situ Hybridization, Fluorescence - veterinary</topic><topic>Metaphase - genetics</topic><topic>Microsatellite Repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ambady, S</creatorcontrib><creatorcontrib>Cheng, H H</creatorcontrib><creatorcontrib>Ponce de León, F A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Poultry science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ambady, S</au><au>Cheng, H H</au><au>Ponce de León, F A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and mapping of microsatellite markers derived from chicken chromosome-specific libraries</atitle><jtitle>Poultry science</jtitle><addtitle>Poult Sci</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>81</volume><issue>11</issue><spage>1644</spage><epage>1646</epage><pages>1644-1646</pages><issn>0032-5791</issn><abstract>Chromosome-specific painting probes and libraries were developed for chicken Macrochromosomes 1, 2, 3, and 4 by chromosome microisolation and microcloning. Fluorescent in situ hybridization results using the painting probes on normal chicken metaphase chromosomes indicated the purity and specificity of each probe. Chromosome-specific libraries for chicken Macrochromosomes 1, 2, 3, and 4 were prepared in a phage vector. Fifty-two additional unique microsatellite markers of the (AC)n type were developed from these chromosome-specific libraries. These markers were mapped on the East Lansing reference population to increase the marker density on the four macrochromosomes. Results of the current study suggest that development of markers from chromosome-specific libraries is very useful for constructing high-density linkage maps for chicken macrochromosomes.</abstract><cop>England</cop><pmid>12455590</pmid><doi>10.1093/ps/81.11.1644</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Poultry science, 2002-11, Vol.81 (11), p.1644-1646 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Chickens - genetics Chromosome Mapping - veterinary Cloning, Molecular DNA Primers - chemistry DNA Probes - chemistry Gene Library Genetic Linkage In Situ Hybridization, Fluorescence - veterinary Metaphase - genetics Microsatellite Repeats |
| title | Development and mapping of microsatellite markers derived from chicken chromosome-specific libraries |
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