Crystallization and preliminary X-ray diffraction studies of NusG, a protein shared by the transcription and translation machines

N‐utilization factor G (NusG) from Aquifex aeolicus (Aa) was overexpressed in Escherichia coli, purified and crystallized using the hanging‐drop vapor‐diffusion technique. The drops consisted of 2.5 µl protein solution (∼30 mg ml−1 in 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 2 mM EDTA and 10 mM DTT) and...

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