Augmentation of Oxygen Transport by Various Hemoglobins as Determined by Pulsed Field Gradient NMR
Diffusion of oxyhemoglobin has been shown to augment the oxygen transport inside the red blood cells. Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coeffic...
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| Veröffentlicht in: | Microvascular research 2002-11, Vol.64 (3), p.463-475 |
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| creator | Budhiraja, Vikas Hellums, David J. Post, Jan F.M. |
| description | Diffusion of oxyhemoglobin has been shown to augment the oxygen transport inside the red blood cells. Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerized and unpolymerized bovine hemoglobin (Hb) and several other proteins were measured using this technique. The Hb diffusion coefficient was used to determine the effective permeability of oxygen and augmentation of oxygen transport through samples of Hb solutions due to diffusion of oxyhemoglobin. The values compared well with our previous diffusion cell measurements of effective diffusivity and augmentation. Our NMR studies show that even at low concentrations the augmentation of oxygen transport due to diffusion can be significant. The PFG NMR technique can thus provide an accurate and easy method for measuring augmentation of oxygen transport, especially in dilute samples of Hb. The results on polyhemoglobin and high-molecular-weight hemoglobin are of both basic interest and practical value in assessing the promise and performance of hemoglobin-based blood substitutes. |
| doi_str_mv | 10.1006/mvre.2002.2449 |
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Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerized and unpolymerized bovine hemoglobin (Hb) and several other proteins were measured using this technique. The Hb diffusion coefficient was used to determine the effective permeability of oxygen and augmentation of oxygen transport through samples of Hb solutions due to diffusion of oxyhemoglobin. The values compared well with our previous diffusion cell measurements of effective diffusivity and augmentation. Our NMR studies show that even at low concentrations the augmentation of oxygen transport due to diffusion can be significant. The PFG NMR technique can thus provide an accurate and easy method for measuring augmentation of oxygen transport, especially in dilute samples of Hb. The results on polyhemoglobin and high-molecular-weight hemoglobin are of both basic interest and practical value in assessing the promise and performance of hemoglobin-based blood substitutes.</description><identifier>ISSN: 0026-2862</identifier><identifier>EISSN: 1095-9319</identifier><identifier>DOI: 10.1006/mvre.2002.2449</identifier><identifier>PMID: 12453440</identifier><identifier>CODEN: MIVRA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; augmentation of oxygen transport ; Biological and medical sciences ; Biological Transport ; blood substitutes ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Cattle ; Diffusion ; hemoglobin ; Hemoglobins - chemistry ; Hemoglobins - metabolism ; Humans ; Magnetic Resonance Spectroscopy ; Medical sciences ; Myoglobin - chemistry ; Oligochaeta ; Ovalbumin - chemistry ; Oxygen - metabolism ; oxygen diffusion ; oxygen transport ; polymerized bovine hemoglobin ; Polymers - chemistry ; pulsed field gradient nuclear magnetic resonance (NMR) ; Temperature ; Transfusions. Complications. Transfusion reactions. 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Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerized and unpolymerized bovine hemoglobin (Hb) and several other proteins were measured using this technique. The Hb diffusion coefficient was used to determine the effective permeability of oxygen and augmentation of oxygen transport through samples of Hb solutions due to diffusion of oxyhemoglobin. The values compared well with our previous diffusion cell measurements of effective diffusivity and augmentation. Our NMR studies show that even at low concentrations the augmentation of oxygen transport due to diffusion can be significant. The PFG NMR technique can thus provide an accurate and easy method for measuring augmentation of oxygen transport, especially in dilute samples of Hb. The results on polyhemoglobin and high-molecular-weight hemoglobin are of both basic interest and practical value in assessing the promise and performance of hemoglobin-based blood substitutes.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>augmentation of oxygen transport</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>blood substitutes</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Cattle</subject><subject>Diffusion</subject><subject>hemoglobin</subject><subject>Hemoglobins - chemistry</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Medical sciences</subject><subject>Myoglobin - chemistry</subject><subject>Oligochaeta</subject><subject>Ovalbumin - chemistry</subject><subject>Oxygen - metabolism</subject><subject>oxygen diffusion</subject><subject>oxygen transport</subject><subject>polymerized bovine hemoglobin</subject><subject>Polymers - chemistry</subject><subject>pulsed field gradient nuclear magnetic resonance (NMR)</subject><subject>Temperature</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtPxCAQh4nR6Pq4ejRc9NYVKKXlaHwnvmLUK6Ew3WDaskJr3P9emt3EkycmzDe_zHwIHVMyp4SI8-47wJwRwuaMc7mFZpTIIpM5ldtolr5FxirB9tB-jJ-EUFpItov2KONFzjmZofpiXHTQD3pwvse-wc8_qwX0-C3oPi59GHC9wh86OD9GfAedX7S-dn3EOuIrGCB0rgc7QS9jG1N146C1-DZo61Isfnp8PUQ7jU69o817gN5vrt8u77KH59v7y4uHzOQlGTIjjGC10JxVXNZG6EZCyUxumyLdUXLBjDW5lI0FDdZKQwtbVNVESagJzw_Q2Tp3GfzXCHFQnYsG2lb3kLZXJStpulkkcL4GTfAxBmjUMrhOh5WiRE1W1WRVTVbVZDUNnGySx7oD-4dvNCbgdAPoaHTbJHnGxT-O50VBBEtcteYgefh2EFQ0SZMB6wKYQVnv_tvhF9MSlFA</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Budhiraja, Vikas</creator><creator>Hellums, David J.</creator><creator>Post, Jan F.M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Augmentation of Oxygen Transport by Various Hemoglobins as Determined by Pulsed Field Gradient NMR</title><author>Budhiraja, Vikas ; Hellums, David J. ; Post, Jan F.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-c6c62b6a42849bc6af9e72c3df50027462cdc399fdeaedd9c15d588f9e79eb043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>augmentation of oxygen transport</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>blood substitutes</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Cattle</topic><topic>Diffusion</topic><topic>hemoglobin</topic><topic>Hemoglobins - chemistry</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Medical sciences</topic><topic>Myoglobin - chemistry</topic><topic>Oligochaeta</topic><topic>Ovalbumin - chemistry</topic><topic>Oxygen - metabolism</topic><topic>oxygen diffusion</topic><topic>oxygen transport</topic><topic>polymerized bovine hemoglobin</topic><topic>Polymers - chemistry</topic><topic>pulsed field gradient nuclear magnetic resonance (NMR)</topic><topic>Temperature</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Budhiraja, Vikas</creatorcontrib><creatorcontrib>Hellums, David J.</creatorcontrib><creatorcontrib>Post, Jan F.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Budhiraja, Vikas</au><au>Hellums, David J.</au><au>Post, Jan F.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Augmentation of Oxygen Transport by Various Hemoglobins as Determined by Pulsed Field Gradient NMR</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>64</volume><issue>3</issue><spage>463</spage><epage>475</epage><pages>463-475</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><coden>MIVRA6</coden><abstract>Diffusion of oxyhemoglobin has been shown to augment the oxygen transport inside the red blood cells. Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerized and unpolymerized bovine hemoglobin (Hb) and several other proteins were measured using this technique. The Hb diffusion coefficient was used to determine the effective permeability of oxygen and augmentation of oxygen transport through samples of Hb solutions due to diffusion of oxyhemoglobin. The values compared well with our previous diffusion cell measurements of effective diffusivity and augmentation. Our NMR studies show that even at low concentrations the augmentation of oxygen transport due to diffusion can be significant. The PFG NMR technique can thus provide an accurate and easy method for measuring augmentation of oxygen transport, especially in dilute samples of Hb. The results on polyhemoglobin and high-molecular-weight hemoglobin are of both basic interest and practical value in assessing the promise and performance of hemoglobin-based blood substitutes.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>12453440</pmid><doi>10.1006/mvre.2002.2449</doi><tpages>13</tpages></addata></record> |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals augmentation of oxygen transport Biological and medical sciences Biological Transport blood substitutes Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Cattle Diffusion hemoglobin Hemoglobins - chemistry Hemoglobins - metabolism Humans Magnetic Resonance Spectroscopy Medical sciences Myoglobin - chemistry Oligochaeta Ovalbumin - chemistry Oxygen - metabolism oxygen diffusion oxygen transport polymerized bovine hemoglobin Polymers - chemistry pulsed field gradient nuclear magnetic resonance (NMR) Temperature Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Augmentation of Oxygen Transport by Various Hemoglobins as Determined by Pulsed Field Gradient NMR |
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